BLOCK-iT™ RNAi Designer

The easiest way to design effective RNAi molecules for great results
 
See also:
BLOCK-iT™ RNAi Express: Simplified online ordering of pre-designed and validated Stealth Select RNAi™ siRNA.
 
Synthetics for in vivo RNAi
 
PlateSelect™: Order made-on-demand RNAi in customizable plate format at 1nmole scale!
 
 
 Target Design Options:  Stealth RNAi™ siRNA siRNA miR RNAi shRNA siRNA to Stealth RNAi™ siRNA siRNA to shRNA
 
Find out more about Stealth RNAi™ siRNA, the next generation RNAi molecule or read about the benefits of BLOCK-iT™ siRNA.     How to Order
 
Step 1: Enter an accession number or provide a nucleotide sequence
 
Accession number:
OR
Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information
Step 2: If you entered an accession number in Step 1, select regions for target design
 
Open reading frame (ORF)
5' UTR
3' UTR
Step 3: Choose database for Blast
  NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on May 01, 2008 and the design output reflects the most up-to-date designs.
Step 4: Choose minimum and maximum G/C percentage
 
Minimum G/C percentage: Maximum G/C percentage:  
Step 5: Select siRNA design options and click "RNAi Design" to design siRNA.
 
Default motif pattern: P: Proprietary (Recommended)
Tuschl's motif pattern: A: AA(N19)TT B: NA(N19)NN C: NA(RN17Y)NN D: NA(N18Y)NN
       
Guarantee: The BLOCK-iT™ RNAi Designer is such an effective tool for the design of siRNAs that if you order the three best siRNA sequences designed by the BLOCK-iT™ RNAi Designer, we guarantee that two of them will give greater than 70% knockdown of mRNA, given that the transfection efficiency in your experiment is at least 80%. If two or more fail to knock down your target RNA by at least 70% under these conditions, Invitrogen will design and ship a fourth siRNA to your target for free*.

*Please contact Invitrogen Technical Services to take advantage of this offer (800-955-6288 ext 2). Please be prepared to fax or email your order reference number, oligo sequences and data showing your transfection efficiency and knockdown. This offer is good for one free duplex per target.

 
How to Order: You must have an online account with Invitrogen to order custom oligos. After your oligos have been designed, you must enter a valid user name and password to send them to the ordering system. If you do not already have an account, click user registration or contact your local representative to set up an account.
 
If you already know your RNAi oligo sequence: Order oligos online at Order Custom Primers or by e-mail/Fax.