Invitrogen

Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience™

FIGURE: 1 / 14

Ki-67 Antibody (11-5699-42) in Flow

Intracellular staining of normal human peripheral blood cells stimulated for 2-day with immobilized Anti-Human CD3 (Product # 16-0037-81) with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Anti-Human Ki-67 FITC (right). Staining was performed using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00).

Ki-67 Antibody in Immunocytochemistry (ICC/IF)

Ki-67 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-5699-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 15 publications 15 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

20Raj1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10687464

Product Specific Information

Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.

This monoclonal antibody 20Raj1 recognizes canine Ki-67.

Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This 20Raj1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered human cytokine/antibody fusion proteins expand regulatory T cells and confer autoimmune disease protection.

Cell reports

VanDyke D,Iglesias M,Tomala J,Young A,Smith J,Perry JA,Gebara E,Cross AR,Cheung LS,Dykema AG,Orcutt-Jahns BT,Henclová T,Golias J,Balolong J,Tomasovic LM,Funda D,Meyer AS,Pardoll DM,Hester J,Issa F,Hunter CA,Anderson MS,Bluestone JA,Raimondi G,Spangler JB

11-5699-42 was used in Flow Cytometry to provide a roadmap for IC design and establish a Treg-biased immunotherapy that could be clinically translated for autoimmune disease treatment.

Tue Oct 18 00:00:00 EDT 2022

Protein/AS01_B vaccination elicits stronger, more Th2-skewed antigen-specific human T follicular helper cell responses than heterologous viral vectors.

Cell reports. Medicine

Nielsen CM,Ogbe A,Pedroza-Pacheco I,Doeleman SE,Chen Y,Silk SE,Barrett JR,Elias SC,Miura K,Diouf A,Bardelli M,Dabbs RA,Barfod L,Long CA,Haynes BF,Payne RO,Minassian AM,Bradley T,Draper SJ,Borrow P

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Flow Cytometry

Tue Mar 16 00:00:00 EDT 2021

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

11-5699 was used in Flow cytometry/Cell sorting to suggest modifications to the costimulatory domains of CAR-T cells can enable longer persistence and thereby improve antitumor response.

Mon Jun 01 00:00:00 EDT 2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

11-5699 was used in Flow cytometry/Cell sorting to suggest modifications to the costimulatory domains of CAR-T cells can enable longer persistence and thereby improve antitumor response.

Mon Jun 01 00:00:00 EDT 2020

Human Liver Memory CD8⁺ T Cells Use Autophagy for Tissue Residence.

Cell reports

Swadling L,Pallett LJ,Diniz MO,Baker JM,Amin OE,Stegmann KA,Burton AR,Schmidt NM,Jeffery-Smith A,Zakeri N,Suveizdyte K,Froghi F,Fusai G,Rosenberg WM,Davidson BR,Schurich A,Simon AK,Maini MK

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Flow Cytometry

Tue Jan 21 00:00:00 EST 2020

Functional Study of the Retrotransposon-Derived Human PEG10 Protease.

International journal of molecular sciences

Golda M,Mótyán JA,Mahdi M,Tőzsér J

11-5699 was used in Flow cytometry/Cell sorting to hypothesize that PRPEG10 plays an important role in the function of this retroviral remnant, mediating the proliferation of cells and possibly implicating it in the inhibition of apoptosis.

Tue Mar 31 00:00:00 EDT 2020

Functional Study of the Retrotransposon-Derived Human PEG10 Protease.

International journal of molecular sciences

Golda M,Mótyán JA,Mahdi M,Tőzsér J

Tue Mar 31 00:00:00 EDT 2020

Inhibition of two-pore channels in antigen-presenting cells promotes the expansion of TNFR2-expressing CD4⁺Foxp3⁺ regulatory T cells.

Science advances

He T,Yang D,Li XQ,Jiang M,Islam MS,Chen S,Chen Y,Yang Y,Chou CK,Trivett AL,Oppenheim JJ,Chen X

11-5699-42 was used in Flow Cytometry to suggest that the therapeutic potential of TPC inhibitors is mediated by expansion of TNFR2-expressing Tregs and elucidate the basis of clinical use in the treatment of autoimmune and other inflammatory diseases.

Tue Sep 01 00:00:00 EDT 2020

Analysis of CAR-Mediated Tonic Signaling.

Methods in molecular biology (Clifton, N.J.)

Calderon H,Mamonkin M,Guedan S

11-5699-42 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry to identify tonic signaling driven by CARs during primary T cell ex vivo expansion.

Thu Dec 31 00:00:00 EST 2020

Involvement of TWEAK and the NF-κB signaling pathway in lupus nephritis.

Experimental and therapeutic medicine

Sun F,Teng J,Yu P,Li W,Chang J,Xu H

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency.

Scientific reports

Nguyen HT,Noguchi S,Sugie K,Matsuo Y,Nguyen CTH,Koito H,Shiojima I,Nishino I,Tsukaguchi H

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Streptomyces Levis ABRIINW111 Inhibits SW480 Cells Growth by Apoptosis Induction.

Advanced pharmaceutical bulletin

Faramarzian Azimi Maragheh B,Fatourachi P,Mohammadi SM,Valipour B,Behtari M,Dehnad A,Nozad Charoudeh H

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Flow Cytometry

Thu Nov 01 00:00:00 EDT 2018

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

11-5699 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage.

Scientific reports

Christoforou N,Chakraborty S,Kirkton RD,Adler AF,Addis RC,Leong KW

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Immunofluorescence

Tue Jan 10 00:00:00 EST 2017

Impact of (chemo)radiotherapy on immune cell composition and function in cervical cancer patients.

Oncoimmunology

van Meir H,Nout RA,Welters MJ,Loof NM,de Kam ML,van Ham JJ,Samuels S,Kenter GG,Cohen AF,Melief CJ,Burggraaf J,van Poelgeest MI,van der Burg SH

11-5699 was used in Flow cytometry/Cell sorting to evaluate the impact of standardradiotherapy on the immune system of cervical cancer patients.

Sat Apr 09 00:00:00 EDT 2022

S100A6 Regulates Endothelial Cell Cycle Progression by Attenuating Antiproliferative Signal Transducers and Activators of Transcription 1 Signaling.

Arteriosclerosis, thrombosis, and vascular biology

Lerchenmüller C,Heißenberg J,Damilano F,Bezzeridis VJ,Krämer I,Bochaton-Piallat ML,Hirschberg K,Busch M,Katus HA,Peppel K,Rosenzweig A,Busch H,Boerries M,Most P

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Immunocytochemistry

Thu Sep 01 00:00:00 EDT 2016

Characterization of Human CD8 T Cell Responses in Dengue Virus-Infected Patients from India.

Journal of virology

Chandele A,Sewatanon J,Gunisetty S,Singla M,Onlamoon N,Akondy RS,Kissick HT,Nayak K,Reddy ES,Kalam H,Kumar D,Verma A,Panda H,Wang S,Angkasekwinai N,Pattanapanyasat K,Chokephaibulkit K,Medigeshi GR,Lodha R,Kabra S,Ahmed R,Murali-Krishna K

11569942 was used in flow cytometry to characterize CD8 positive T cell subsets in dengue patients from India and Thailand

Thu Dec 15 00:00:00 EST 2016

Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry.

Cancer immunology, immunotherapy : CII

Santegoets SJ,Dijkgraaf EM,Battaglia A,Beckhove P,Britten CM,Gallimore A,Godkin A,Gouttefangeas C,de Gruijl TD,Koenen HJ,Scheffold A,Shevach EM,Staats J,Taskén K,Whiteside TL,Kroep JR,Welters MJ,van der Burg SH

11-5699 was used in Flow cytometry/Cell sorting to exhibit a novel strategy and system for the context-dependent analysis of Tregs.

Thu Oct 01 00:00:00 EDT 2015

Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.

Journal of immunology (Baltimore, Md. : 1950)

Tsai CY,Liong KH,Gunalan MG,Li N,Lim DS,Fisher DA,MacAry PA,Leo YS,Wong SC,Puan KJ,Wong SB

11-5699 was used in Flow cytometry/Cell sorting to investigate the markers of cellular innate immunity against dengue virus, specifically how type I IFNs and IL-18 regulate activation, proliferation and degranulation of T cells against dendritic cells in human patients with dengue virus.

Wed Apr 15 00:00:00 EDT 2015

A phase 1/2 study combining gemcitabine, Pegintron and p53 SLP vaccine in patients with platinum-resistant ovarian cancer.

Oncotarget

Dijkgraaf EM,Santegoets SJ,Reyners AK,Goedemans R,Nijman HW,van Poelgeest MI,van Erkel AR,Smit VT,Daemen TA,van der Hoeven JJ,Melief CJ,Welters MJ,Kroep JR,van der Burg SH

11-5699 was used in Flow cytometry/Cell sorting to study the feasibility and immunogenicity of treating patients with platinum-resistant p53-positive ovarian cancer using combinatorial regimens.

Tue Oct 13 00:00:00 EDT 2015

Biliary tree stem cells, precursors to pancreatic committed progenitors: evidence for possible life-long pancreatic organogenesis.

Stem cells (Dayton, Ohio)

Wang Y,Lanzoni G,Carpino G,Cui CB,Dominguez-Bendala J,Wauthier E,Cardinale V,Oikawa T,Pileggi A,Gerber D,Furth ME,Alvaro D,Gaudio E,Inverardi L,Reid LM

Published figure using Ki-67 monoclonal antibody (Product # 11-5699-42) in Immunofluorescence

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

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Gene Aliases: KIA; MIB-; MIB-1; MKI67; PPP1R105

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UniProt ID: (Human) P46013

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Entrez Gene ID: (Dog) 100686578, (Human) 4288

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Function(s)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding

Process(es)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience™

Ki-67 Antibody (11-5699-42) in Flow

Product Details

11-5699-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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