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Invitrogen
Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™
FIGURE: 1 / 1
Mouse IgG2a kappa Isotype Control (12-4724-42) in Flow
Flow cytometric analysis of toll-like receptor 3 (TLR3) in immune cells from ileal Peyer's patches (PP). Mononuclear cells were isolated from adult swine PP and incubated with antibodies for CD45, CD172a, CD11R1, CD21, CD4 and CD8. (A) Histograms show flow cytometric analysis for TLR3 staining as follows: intracellular (open histogram), cell surface (broken lines) and isotype-matched controls (shaded histog... View More
Product Details
12-4724-42
Applications
Tested Dilution
Flow Cytometry (Flow)
5 µL (0.5 µg)/test
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBM2a
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1603322
Product Specific Information
Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.
Applications Reported:This mouse IgG2a isotype control has been reported for use in cell surface and intracellular flow cytometric analysis.
Applications Tested: Has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at test size: 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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