Invitrogen

Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

14 Published Figures

19 References

View all (58) Occludin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Occludin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

Occludin Antibody (331588) in ICC/IF

Knockout of Occludin was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR777079_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on Caco-2 wild type cells (panel a,d), Caco-2 Cas9 control cells (panel b,e) and Caco-2 Occludin KO cells (panel c, f). Cells were fixed, permeabilized, and labeled with Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488 (Product # 331588, 1:100). Nuclei (blue) were stained using ProLong™ Diamond Anti... View More

Occludin Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

331588

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-5 µg/mL

View 10 publications 10 publications

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Bovine, Dog, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

OC-3F10

Immunogen

GST-fusion protein consisting of the C-terminal region (~150 aa) of human occludin.

View immunogen

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 4.0mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532185

Product Specific Information

Reactivity of this antibody with the occludin protein has been confirmed by immunofluorescence and western blotting (unconjugated). This antibody reacts specifically with mammalian occludin. Tissues/lysates that were tested with this antibody include T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma), MTE7B (Mouse), and rat liver.

Target Information

Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SERPIN-Derived Small Peptide (SP16) as a Potential Therapeutic Agent against HIV-Induced Inflammatory Molecules and Viral Replication in Cells of the Central Nervous System.

Cells

Soler Y,Rodriguez M,Austin D,Gineste C,Gelber C,El-Hage N

331588 was used in Immunohistochemistry-immunofluorescence to show that SP16 interaction with LRP-1 and binding lead to: (i) down-regulation in the expression levels of nuclear factor-kappa beta (NF-κB); and (ii) up-regulation in the expression levels of Akt.

Wed Feb 15 00:00:00 EST 2023

SERPIN-Derived Small Peptide (SP16) as a Potential Therapeutic Agent against HIV-Induced Inflammatory Molecules and Viral Replication in Cells of the Central Nervous System.

Cells

Soler Y,Rodriguez M,Austin D,Gineste C,Gelber C,El-Hage N

Wed Feb 15 00:00:00 EST 2023

A cellular and spatial map of the choroid plexus across brain ventricles and ages.

Cell

Dani N,Herbst RH,McCabe C,Green GS,Kaiser K,Head JP,Cui J,Shipley FB,Jang A,Dionne D,Nguyen L,Rodman C,Riesenfeld SJ,Prochazka J,Prochazkova M,Sedlacek R,Zhang F,Bryja V,Rozenblatt-Rosen O,Habib N,Regev A,Lehtinen MK

331588 was used in Immunohistochemistry to reveal cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.

Thu May 27 00:00:00 EDT 2021

A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications.

Molecular therapy. Methods & clinical development

Munis AM,Hyde SC,Gill DR

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Fri Mar 12 00:00:00 EST 2021

A human surfactant B deficiency air-liquid interface cell culture model suitable for gene therapy applications.

Molecular therapy. Methods & clinical development

Munis AM,Hyde SC,Gill DR

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Fri Mar 12 00:00:00 EST 2021

Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.

Frontiers in bioengineering and biotechnology

Jing B,Wang ZA,Zhang C,Deng Q,Wei J,Luo Y,Zhang X,Li J,Du Y

331588 was used in Immunocytochemistry-immunoflourescence to develop a novel gut-vessel microfluidic model to study host-microbial interactions.

Mon Sep 28 00:00:00 EDT 2020

Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.

Frontiers in bioengineering and biotechnology

Jing B,Wang ZA,Zhang C,Deng Q,Wei J,Luo Y,Zhang X,Li J,Du Y

331588 was used in Immunocytochemistry-immunoflourescence to develop a novel gut-vessel microfluidic model to study host-microbial interactions.

Mon Sep 28 00:00:00 EDT 2020

ApoE (Apolipoprotein E) in Brain Pericytes Regulates Endothelial Function in an Isoform-Dependent Manner by Modulating Basement Membrane Components.

Arteriosclerosis, thrombosis, and vascular biology

Yamazaki Y,Shinohara M,Yamazaki A,Ren Y,Asmann YW,Kanekiyo T,Bu G

331588 was used in Immunocytochemistry-immunofluorescence to show that brain pericytes, the mural cells of the capillary walls, differentially modulate endothelial cell phenotype in an apoE isoform-dependent manner.

Wed Jan 01 00:00:00 EST 2020

A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability.

Frontiers in oncology

Sherman H,Rossi AE

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

A Novel Three-Dimensional Glioma Blood-Brain Barrier Model for High-Throughput Testing of Tumoricidal Capability.

Frontiers in oncology

Sherman H,Rossi AE

Published figure using Occludin monoclonal antibody (Product # 331588) in Immunocytochemistry

Thu Oct 01 00:00:00 EDT 2020

ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.

Molecular human reproduction

DaSilva-Arnold SC,Kuo CY,Davra V,Remache Y,Kim PCW,Fisher JP,Zamudio S,Al-Khan A,Birge RB,Illsley NP

Fri Feb 01 00:00:00 EST 2019

Involvement of CYP1B1 in interferon γ-induced alterations of epithelial barrier integrity.

British journal of pharmacology

Alhouayek M,Gouveia-Figueira S,Hammarström ML,Fowler CJ

331588 was used in Immunohistochemistry-immunofluorescence to suggest that increased expression of CYP1B1 is involved in the effects of IFNγ on epithelial permeability.

Thu Mar 01 00:00:00 EST 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

Apelin-13 Is an Early Promoter of Cytoskeleton and Tight Junction in Diabetic Macular Edema via PI-3K/Akt and MAPK/Erk Signaling Pathways.

BioMed research international

Li Y,Bai YJ,Jiang YR,Yu WZ,Shi X,Chen L,Feng J,Sun GB

331588 was used in Immunofluorescence in immunohistochemistry on frozen tissues to investigate the effects and mechanism of apelin-13 in vascular permeability during diabetic macular oedema.

Mon Oct 01 00:00:00 EDT 2018

The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability.

The Journal of biological chemistry

Ramos CJ,Lin C,Liu X,Antonetti DA

331588 was used in Immunocytochemistry-Immunofluorescence to determine the role of the cAMP dependent guanine nucleotide exchange factor-Rap 1 pathway in retinal vascular permeability.

Fri Jan 12 00:00:00 EST 2018

The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability.

The Journal of biological chemistry

Ramos CJ,Lin C,Liu X,Antonetti DA

331588 was used in Immunocytochemistry-Immunofluorescence to determine the role of the cAMP dependent guanine nucleotide exchange factor-Rap 1 pathway in retinal vascular permeability.

Fri Jan 12 00:00:00 EST 2018

The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability.

The Journal of biological chemistry

Ramos CJ,Lin C,Liu X,Antonetti DA

331588 was used in Immunocytochemistry-Immunofluorescence to determine the role of the cAMP dependent guanine nucleotide exchange factor-Rap 1 pathway in retinal vascular permeability.

Fri Jan 12 00:00:00 EST 2018

Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1.

Proceedings of the National Academy of Sciences of the United States of America

Yanagida K,Liu CH,Faraco G,Galvani S,Smith HK,Burg N,Anrather J,Sanchez T,Iadecola C,Hla T

331588 was used in immunohistochemistry - free floating to describe a role for sphingosine 1-phosphate receptor-1 in regulating blood brain barrier permeability

Tue Apr 25 00:00:00 EDT 2017

Electro-Magnetic Nano-Particle Bound Beclin1 siRNA Crosses the Blood-Brain Barrier to Attenuate the Inflammatory Effects of HIV-1 Infection in Vitro.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology

Rodriguez M,Kaushik A,Lapierre J,Dever SM,El-Hage N,Nair M

331588 was used in immunocytochemistry to analyze how to cease the inflammatory effects of HIV-1 infection in vitro by electro-magnetic nano-particle bound beclin1 siRNA that crosses the blood-brain barrier

Wed Mar 01 00:00:00 EST 2017

Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier.

Nature communications

Campos Y,Qiu X,Gomero E,Wakefield R,Horner L,Brutkowski W,Han YG,Solecki D,Frase S,Bongiovanni A,d'Azzo A

331588 was used in immunohistochemistry - frozen section to analyze preservation of epithelial polarity and epithelial barrier by alix-mediated assembly of the actomyosin-tight junction polarity complex

Thu Jun 23 00:00:00 EDT 2016

Temporal Monitoring of Differentiated Human Airway Epithelial Cells Using Microfluidics.

PloS one

Blume C,Reale R,Held M,Millar TM,Collins JE,Davies DE,Morgan H,Swindle EJ

331588 was used in immunocytochemistry to study the use of microfluidics in temporal monitoring of differentiated human airway epithelial cells

Wed Jun 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; tight junction structural protein

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Gene Aliases: AI503564; BLCPMG; Ocl; OCLN; PPP1R115

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UniProt ID: (Dog) Q28269, (Human) Q16625, (Mouse) Q61146, (Rat) Q6P6T5

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Entrez Gene ID: (Dog) 403844, (Human) 100506658, (Mouse) 18260, (Rat) 83497

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Function(s)

protein binding thiopurine S-methyltransferase activity protein domain specific binding S-adenosylmethionine-dependent methyltransferase activity structural molecule activity general transcription factor

Process(es)

positive regulation of lamellipodium assembly positive regulation of microtubule polymerization bicellular tight junction assembly positive regulation of wound healing protein localization to cell leading edge protein complex assembly methylation cell-cell junction organization S-adenosylhomocysteine metabolic process S-adenosylmethionine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Occludin Monoclonal Antibody (OC-3F10), Alexa Fluor™ 488

Occludin Antibody (331588) in ICC/IF

Product Details

331588

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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