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Invitrogen
Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 570, eBioscience™
Product Details
41-4321-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
5-10 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
1-5 µg/mL
Immunocytochemistry (ICC/IF)
1-5 µg/mL
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBR2a
Conjugate
Excitation/Emission Max
556/569 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11219683
Product Specific Information
Description: The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.
Applications Reported: This eBR2a antibody has been reported for use in immunohistochemical and immunocytochemical staining.
Applications Tested: This Rat IgG2a Isotype Control eFluor® 570 has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells and by immunohistochemistry (IHC-P) on formalin-fixed paraffin embedded human tissue. Use isotype control at the same concentration as experimental antibody.
Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.
Excitation: 555 nm; Emission: 570 nm
Target Information
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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