Invitrogen

CD19 Monoclonal Antibody (eBio1D3 (1D3)), APC-eFluor™ 780, eBioscience™

FIGURE: 1 / 64

CD19 Antibody (47-0193-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) and 0.06 µg of Anti-Mouse CD19 APC-eFluor® 780 (Product # 47-0193-82) (left) or 0.06 µg of Anti-Mouse CD19 APC-eFluor® 780 (right). Total viable cells were used for analysis.

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Product Details

47-0193-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 76 publications 76 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio1D3 (1D3)

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853189

Product Specific Information

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cissus quadrangularis (Hadjod) Inhibits RANKL-Induced Osteoclastogenesis and Augments Bone Health in an Estrogen-Deficient Preclinical Model of Osteoporosis Via Modulating the Host Osteoimmune System.

Cells

Azam Z,Sapra L,Baghel K,Sinha N,Gupta RK,Soni V,Saini C,Mishra PK,Srivastava RK

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Jan 04 00:00:00 EST 2023

IL-4 and helminth infection downregulate MINCLE-dependent macrophage response to mycobacteria and Th17 adjuvanticity.

eLife

Schick J,Altunay M,Lacorcia M,Marschner N,Westermann S,Schluckebier J,Schubart C,Bodendorfer B,Christensen D,Alexander C,Wirtz S,Voehringer D,da Costa CP,Lang R

47-0193-82 was used in Flow cytometry/Cell sorting to demonstrate downregulation of MINCLE expression on monocytes and macrophages by IL-4 as a possible mechanism of thwarted Th17 vaccination responses by underlying helminth infection.

Wed Feb 08 00:00:00 EST 2023

Plasmodium infection disrupts the T follicular helper cell response to heterologous immunization.

eLife

Fontana MF,Ollmann Saphire E,Pepper M

47-0193-82 was used in Flow cytometry/Cell sorting to provide mechanistic insight into the development of both Plasmodium-specific and heterologous adaptive responses in hosts with malaria.

Mon Jan 30 00:00:00 EST 2023

Role of FGF10/FGFR2b Signaling in Homeostasis and Regeneration of Adult Lacrimal Gland and Corneal Epithelium Proliferation.

Investigative ophthalmology & visual science

Finburgh EN,Mauduit O,Noguchi T,Bu JJ,Abbas AA,Hakim DF,Bellusci S,Meech R,Makarenkova HP,Afshari NA

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Valosin-containing protein (VCP/p97) inhibition reduces viral clearance and induces toxicity associated with muscular damage.

Cell death & disease

Del Rio Oliva M,Basler M

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

High-yield enrichment of mouse small intestine intraepithelial lymphocytes by immunomagnetic depletion of EpCAM⁺ cells.

STAR protocols

Prakhar P,Gonzalez V,Park JH

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Fri Mar 18 00:00:00 EDT 2022

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Functional human IgA targets a conserved site on malaria sporozoites.

Science translational medicine

Tan J,Cho H,Pholcharee T,Pereira LS,Doumbo S,Doumtabe D,Flynn BJ,Schön A,Kanatani S,Aylor SO,Oyen D,Vistein R,Wang L,Dillon M,Skinner J,Peterson M,Li S,Idris AH,Molina-Cruz A,Zhao M,Olano LR,Lee PJ,Roth A,Sinnis P,Barillas-Mury C,Kayentao K,Ongoiba A,Francica JR,Traore B,Wilson IA,Seder RA,Crompton PD

47-0193-82 was used in Flow Cytometry to investigate the circulating IgA response in humans to P. falciparum sporozoites that are injected into the skin by mosquitoes and migrate to the liver via the bloodstream to initiate malaria infection.

Wed Jun 23 00:00:00 EDT 2021

Functional human IgA targets a conserved site on malaria sporozoites.

Science translational medicine

Wed Jun 23 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

47-0193-82 was used in Flow Cytometry to show a persistent accumulation of T cells in mice and human autopsy samples for more than 1 mo after stroke.

Mon Aug 02 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

47-0193-82 was used in Flow Cytometry to show a persistent accumulation of T cells in mice and human autopsy samples for more than 1 mo after stroke.

Mon Aug 02 00:00:00 EDT 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

The gammaherpesvirus 68 viral cyclin facilitates expression of LANA.

PLoS pathogens

Niemeyer BF,Sanford B,Gibson JE,Berger JN,Oko LM,Medina E,Clambey ET,van Dyk LF

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Regulatory B Cells (Bregs) Inhibit Osteoclastogenesis and Play a Potential Role in Ameliorating Ovariectomy-Induced Bone Loss.

Frontiers in immunology

Sapra L,Bhardwaj A,Mishra PK,Garg B,Verma B,Mishra GC,Srivastava RK

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Nov 04 00:00:00 EDT 2021

Comprehensive mutagenesis identifies the peptide repertoire of a p53 T-cell receptor mimic antibody that displays no toxicity in mice transgenic for human HLA-A*0201.

PloS one

Trenevska I,Anderson AP,Bentley C,Hassanali T,Wiblin S,Maguire S,Pezzella F,Banham AH,Li D

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Sep 29 00:00:00 EDT 2021

Type I interferon decreases macrophage energy metabolism during mycobacterial infection.

Cell reports

Olson GS,Murray TA,Jahn AN,Mai D,Diercks AH,Gold ES,Aderem A

47-0193-82 was used in Flow Cytometry to suggest that a type I IFN-mitochondrial feedback loop controls macrophage responses to mycobacteria and that this could contribute to pathogenesis across a range of diseases.

Tue Jun 01 00:00:00 EDT 2021

The ASCIZ-DYNLL1 Axis Is Essential for TLR4-Mediated Antibody Responses and NF-κB Pathway Activation.

Molecular and cellular biology

Liu R,King A,Tarlinton D,Heierhorst J

47-0193-82 was used in Flow cytometry/Cell sorting to identify DYNLL1 as a signal-specific regulator of the NF-κB pathway and indicate that it may act as a universal modulator of TLR4 (and IL-1) signaling with wide-ranging roles in inflammation and immunity.

Mon Nov 22 00:00:00 EST 2021

Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist.

eLife

Glassman CR,Su L,Majri-Morrison SS,Winkelmann H,Mo F,Li P,Pérez-Cruz M,Ho PP,Koliesnik I,Nagy N,Hnizdilova T,Picton LK,Kovar M,Bollyky P,Steinman L,Meyer E,Piehler J,Leonard WJ,Garcia KC

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition.

Cell

Pasciuto E,Burton OT,Roca CP,Lagou V,Rajan WD,Theys T,Mancuso R,Tito RY,Kouser L,Callaerts-Vegh Z,de la Fuente AG,Prezzemolo T,Mascali LG,Brajic A,Whyte CE,Yshii L,Martinez-Muriana A,Naughton M,Young A,Moudra A,Lemaitre P,Poovathingal S,Raes J,De Strooper B,Fitzgerald DC,Dooley J,Liston A

47-0193 was used in Flow cytometry/Cell sorting to investigate the function of brain-resident CD4 T cells.

Thu Aug 06 00:00:00 EDT 2020

Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development.

Developmental cell

Dege C,Fegan KH,Creamer JP,Berrien-Elliott MM,Luff SA,Kim D,Wagner JA,Kingsley PD,McGrath KE,Fehniger TA,Palis J,Sturgeon CM

47-0193 was used in Flow cytometry/Cell sorting to identify an extra-embryonic origin of potently cytotoxic NK cells, suggesting that ontogenic origin is a relevant factor in designing hPSC-derived adoptive immunotherapies.

Mon Apr 20 00:00:00 EDT 2020

Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells.

Cancer immunology research

Wijewarnasuriya D,Bebernitz C,Lopez AV,Rafiq S,Brentjens RJ

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Zdhhc2 Is Essential for Plasmacytoid Dendritic Cells Mediated Inflammatory Response in Psoriasis.

Frontiers in immunology

Zhou B,Yang W,Li W,He L,Lu L,Zhang L,Liu Z,Wang Y,Chao T,Huang R,Gu Y,Jia T,Liu Q,Tian S,Pierre P,Maeda T,Liang Y,Kong E

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Jun 17 00:00:00 EDT 2021

Diversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells.

Nature communications

Lucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson G

47-0193 was used in Flow cytometry/Cell sorting to study the mechanism that controls the development of αβ T cell lineages in the thymus.

Mon May 04 00:00:00 EDT 2020

Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice.

Bio-protocol

Rothchild AC,Mai D,Aderem A,Diercks AH

47-0193 was used in Flow cytometry/Cell sorting to describe a method that uses fluorescent bacteria, a high-dose aerosol infection model, and flow cytometry to track Mtb-infected cells in the lung immediately following aerosol infection and fluorescence-activated cell sorting (FACS) to isolate naïve, bystander, and Mtb-infected cells for downstream applications, including RNA-sequencing.

Wed May 20 00:00:00 EDT 2020

Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B.

PloS one

Parra M,Weitner M,Yang A,Akue A,Liu X,Schmidt T,Allman WR,Akkoyunlu M,Derrick SC

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Pharmacological depletion of microglia and perivascular macrophages prevents Vascular Cognitive Impairment in Ang II-induced hypertension.

Theranostics

Kerkhofs D,van Hagen BT,Milanova IV,Schell KJ,van Essen H,Wijnands E,Goossens P,Blankesteijn WM,Unger T,Prickaerts J,Biessen EA,van Oostenbrugge RJ,Foulquier S

47-0193 was used in Flow cytometry/Cell sorting to support the critical role of brain immune cells in the pathogenesis of hypertension-related cognitive impairment.

Tue Jun 01 00:00:00 EDT 2021

CD103⁺ cDC1 and endogenous CD8⁺ T cells are necessary for improved CD40L-overexpressing CAR T cell antitumor function.

Nature communications

Kuhn NF,Lopez AV,Li X,Cai W,Daniyan AF,Brentjens RJ

47-0193 was used in Flow cytometry/Cell sorting to demonstrate the stimulatory effect of CD40L-overexpressing CAR T cells on innate and adaptive immune cells, and provide a rationale for using CD40L-overexpressing CAR T cells to improve immunotherapy responses.

Wed Dec 02 00:00:00 EST 2020

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production.

Journal of visualized experiments : JoVE

Upadhye A,Marshall M,Garmey JC,Bender TP,McNamara C

47-0193 was used in Flow cytometry/Cell sorting to describe a method to target B-1a cell migration to the bone marrow by retroviral-mediated overexpression of the C-X-C motif chemokine receptor 4 (CXCR4).

Sun May 31 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Virus infection is controlled by hematopoietic and stromal cell sensing of murine cytomegalovirus through STING.

eLife

Piersma SJ,Poursine-Laurent J,Yang L,Barber GN,Parikh BA,Yokoyama WM

47-0193 was used in Flow cytometry/Cell sorting to contrast anti-viral effects of MyD88 versus STING in distinct cell types that are infected with murine CMV (MCMV).

Wed Jul 29 00:00:00 EDT 2020

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

47-0193-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

The methyltransferase PRMT6 attenuates antiviral innate immunity by blocking TBK1-IRF3 signaling.

Cellular & molecular immunology

Zhang H,Han C,Li T,Li N,Cao X

47-0193-82 was used in Flow Cytometry to demonstrates a methyltransferase-independent role for PRMTs.

Tue Oct 01 00:00:00 EDT 2019

Inflammation via myeloid differentiation primary response gene 88 signaling mediates the fibrotic response to implantable synthetic poly(ethylene glycol) hydrogels.

Acta biomaterialia

Amer LD,Saleh LS,Walker C,Thomas S,Janssen WJ,Alper S,Bryant SJ

47-0193 was used in Flow cytometry/Cell sorting to demonstrate that MyD88-mediated inflammation is responsible in part for the formation of the fibrous capsule of the FBR.

Sun Dec 01 00:00:00 EST 2019

NLRX1 inhibits the early stages of CNS inflammation and prevents the onset of spontaneous autoimmunity.

PLoS biology

Gharagozloo M,Mahmoud S,Simard C,Yamamoto K,Bobbala D,Ilangumaran S,Smith MD,Lamontagne A,Jarjoura S,Denault JB,Blais V,Gendron L,Vilariño-Güell C,Sadovnick AD,Ting JP,Calabresi PA,Amrani A,Gris D

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Peritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus.

Frontiers in immunology

Gautam A,Park BK,Kim TH,Akauliya M,Kim D,Maharjan S,Park J,Kim J,Lee H,Park MS,Lee Y,Kwon HJ

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Aug 17 00:00:00 EDT 2020

Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.

Scientific reports

Goldstein JM,Valido A,Lewandowski JP,Walker RG,Mills MJ,Messemer KA,Besseling P,Lee KH,Wattrus SJ,Cho M,Lee RT,Wagers AJ

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Dec 09 00:00:00 EST 2019

Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.

JCI insight

Huang A,Shinde PV,Huang J,Senff T,Xu HC,Margotta C,Häussinger D,Willnow TE,Zhang J,Pandyra AA,Timm J,Weggen S,Lang KS,Lang PA

47-0193 was used in Flow cytometry/Cell sorting to show that progranulin (PGRN) can reduce NK cell cytotoxicity through reduction of NK cell expansion, granzyme B transcription, and NK cell-mediated lysis of target cells.

Thu Sep 05 00:00:00 EDT 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

TET2 Deficiency Causes Germinal Center Hyperplasia, Impairs Plasma Cell Differentiation, and Promotes B-cell Lymphomagenesis.

Cancer discovery

Dominguez PM,Ghamlouch H,Rosikiewicz W,Kumar P,Béguelin W,Fontán L,Rivas MA,Pawlikowska P,Armand M,Mouly E,Torres-Martin M,Doane AS,Calvo Fernandez MT,Durant M,Della-Valle V,Teater M,Cimmino L,Droin N,Tadros S,Motanagh S,Shih AH,Rubin MA,Tam W,Aifantis I,Levine RL,Elemento O,Inghirami G,Green MR,Figueroa ME,Bernard OA,Aoufouchi S,Li S,Shaknovich R,Melnick AM

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

TET2 Deficiency Causes Germinal Center Hyperplasia, Impairs Plasma Cell Differentiation, and Promotes B-cell Lymphomagenesis.

Cancer discovery

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

11 Beta-hydroxysteroid dehydrogenase type 1 regulates synovitis, joint destruction, and systemic bone loss in chronic polyarthritis.

Journal of autoimmunity

Hardy RS,Fenton C,Croft AP,Naylor AJ,Begum R,Desanti G,Buckley CD,Lavery G,Cooper MS,Raza K

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.

Frontiers in immunology

Liu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst J

47-0193 was used in Flow cytometry/Cell sorting to indicate that BIM regulates B cell homeostasis predominantly by limiting the life span of non-activated mature B cells.

Wed May 01 00:00:00 EDT 2019

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

The Role of Allograft Inflammatory Factor-1 in the Effects of Experimental Diabetes on B Cell Functions in the Heart.

Frontiers in cardiovascular medicine

Sarkar A,Shukla SK,Alqatawni A,Kumar A,Addya S,Tsygankov AY,Rafiq K

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.

Stem cell reports

Kauts ML,Rodriguez-Seoane C,Kaimakis P,Mendes SC,Cortés-Lavaud X,Hill U,Dzierzak E

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Jan 09 00:00:00 EST 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

A Gut Commensal-Produced Metabolite Mediates Colonization Resistance to Salmonella Infection.

Cell host & microbe

Jacobson A,Lam L,Rajendram M,Tamburini F,Honeycutt J,Pham T,Van Treuren W,Pruss K,Stabler SR,Lugo K,Bouley DM,Vilches-Moure JG,Smith M,Sonnenburg JL,Bhatt AS,Huang KC,Monack D

47-0193-82 was used in flow cytometry to provide mechanistic understanding into the role of individualized microbial communities in host-to-host variability of pathogen transmission.

Wed Aug 08 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

47-0193 was used in Flow cytometry/Cell sorting to study paediatric acute myeloid leukaemia biology, and provide murine models that offer the potential for developing paediatric specific therapeutic strategies.

Tue Dec 11 00:00:00 EST 2018

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

47-0193 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone.

Thu Jun 01 00:00:00 EDT 2017

A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.

The Journal of clinical investigation

Chang AY,Dao T,Gejman RS,Jarvis CA,Scott A,Dubrovsky L,Mathias MD,Korontsvit T,Zakhaleva V,Curcio M,Hendrickson RC,Liu C,Scheinberg DA

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Fri Jun 30 00:00:00 EDT 2017

Microbial, metabolomic, and immunologic dynamics in a relapsing genetic mouse model of colitis induced by T-synthase deficiency.

Gut microbes

Jacobs JP,Lin L,Goudarzi M,Ruegger P,McGovern DP,Fornace AJ,Borneman J,Xia L,Braun J

47-0193 was used in Flow cytometry/Cell sorting to identify polymorphisms near C1galt1 and its molecular chaperone, Cosmc, were associated with altered composition of the colonic mucosal microbiota.

Mon Jan 02 00:00:00 EST 2017

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Vaccine-induced Th17 cells are established as resident memory cells in the lung and promote local IgA responses.

Mucosal immunology

Christensen D,Mortensen R,Rosenkrands I,Dietrich J,Andersen P

47-0193 was used in Flow cytometry/Cell sorting to demonstrate that subcutaneous priming with a recombinant antigen in a T helper (Th)17-inducing adjuvant followed by airway boosting promotes high and sustained levels of IgA in the lungs.

Sun Jan 01 00:00:00 EST 2017

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Wagner JM,Jaurich H,Wallner C,Abraham S,Becerikli M,Dadras M,Harati K,Duhan V,Khairnar V,Lehnhardt M,Behr B

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Intravenously usable fully serotype 3 oncolytic adenovirus coding for CD40L as an enabler of dendritic cell therapy.

Oncoimmunology

Zafar S,Parviainen S,Siurala M,Hemminki O,Havunen R,Tähtinen S,Bramante S,Vassilev L,Wang H,Lieber A,Hemmi S,de Gruijl T,Kanerva A,Hemminki A

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia.

Cancer discovery

Pylayeva-Gupta Y,Das S,Handler JS,Hajdu CH,Coffre M,Koralov SB,Bar-Sagi D

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Medial HOXA genes demarcate haematopoietic stem cell fate during human development.

Nature cell biology

Dou DR,Calvanese V,Sierra MI,Nguyen AT,Minasian A,Saarikoski P,Sasidharan R,Ramirez CM,Zack JA,Crooks GM,Galic Z,Mikkola HK

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Antigen Targeting to Human HLA Class II Molecules Increases Efficacy of DNA Vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Grodeland G,Fredriksen AB,Løset GÅ,Vikse E,Fugger L,Bogen B

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2016

Protein Translation Activity: A New Measure of Host Immune Cell Activation.

Journal of immunology (Baltimore, Md. : 1950)

Seedhom MO,Hickman HD,Wei J,David A,Yewdell JW

47-0193 was used in Flow cytometry/Cell sorting to describe the in vivo ribopuromycylation method.

Mon Aug 15 00:00:00 EDT 2016

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Jul 27 00:00:00 EDT 2016

Novel CXCL13 transgenic mouse: inflammation drives pathogenic effect of CXCL13 in experimental myasthenia gravis.

Oncotarget

Weiss JM,Robinet M,Aricha R,Cufi P,Villeret B,Lantner F,Shachar I,Fuchs S,Souroujon MC,Berrih-Aknin S,Le Panse R

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2016

Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice.

Scientific reports

Xiong J,Zhou MF,Wang YD,Chen LP,Xu WF,Wang YD,Deng F,Liu SD

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Fri Sep 23 00:00:00 EDT 2016

p21^Cip1 plays a critical role in the physiological adaptation to fasting through activation of PPARα.

Scientific reports

Lopez-Guadamillas E,Fernandez-Marcos PJ,Pantoja C,Muñoz-Martin M,Martínez D,Gómez-López G,Campos-Olivas R,Valverde AM,Serrano M

47-0193 was used in Flow cytometry/Cell sorting to examine the expression of several tumour suppressors upon fasting in mice.

Mon Oct 10 00:00:00 EDT 2016

Cis-regulatory mechanisms governing stem and progenitor cell transitions.

Science advances

Johnson KD,Kong G,Gao X,Chang YI,Hewitt KJ,Sanalkumar R,Prathibha R,Ranheim EA,Dewey CN,Zhang J,Bresnick EH

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Thu Jan 15 00:00:00 EST 2015

B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.

Journal of immunology (Baltimore, Md. : 1950)

Pone EJ,Lam T,Lou Z,Wang R,Chen Y,Liu D,Edinger AL,Xu Z,Casali P

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.

Journal of immunology (Baltimore, Md. : 1950)

Yu M,Chen Y,He Y,Podd A,Fu G,Wright JA,Kleiman E,Khan WN,Wen R,Wang D

Published figure using CD19 monoclonal antibody (Product # 47-0193-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Impaired innate immunity in mice deficient in interleukin-1 receptor-associated kinase 4 leads to defective type 1 T cell responses, B cell expansion, and enhanced susceptibility to infection with Toxoplasma gondii.

Infection and immunity

Béla SR,Dutra MS,Mui E,Montpetit A,Oliveira FS,Oliveira SC,Arantes RM,Antonelli LR,McLeod R,Gazzinelli RT

47-0193 was used in Flow cytometry/Cell sorting to investigate the role of IRAK4 in Toxoplasma gondii infection, showing that deficiency leads to impaired innate immunity.

Sat Dec 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12

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Gene Aliases: AW495831; Cd19

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UniProt ID: (Mouse) P25918

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Entrez Gene ID: (Mouse) 12478

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Process(es)

chromosome breakage B cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol

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CD19 Monoclonal Antibody (eBio1D3 (1D3)), APC-eFluor™ 780, eBioscience™

CD19 Antibody (47-0193-82) in Flow

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47-0193-82

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