Invitrogen

CD45 Monoclonal Antibody (HI30), APC-eFluor™ 780, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

17 References

View all (406) CD45 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 2

CD45 Antibody (47-0459-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD45 APC-eFluor® 780 (purple histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

Product Details

47-0459-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HI30

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1944368

Product Specific Information

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Plasmatic MMP9 released from tumor-infiltrating neutrophils is predictive for bevacizumab efficacy in glioblastoma patients: an AVAglio ancillary study.

Acta neuropathologica communications

Jiguet-Jiglaire C,Boissonneau S,Denicolai E,Hein V,Lasseur R,Garcia J,Romain S,Appay R,Graillon T,Mason W,Carpentier AF,Brandes AA,Ouafik L',Wick W,Baaziz A,Gigan JP,Argüello RJ,Figarella-Branger D,Chinot O,Tabouret E

47-0459-42 was used in Flow Cytometry to assess the predictive value of matrix metalloproteinase 2 (MMP2) and MMP9 in a randomized phase III trial in patients with newly diagnosed glioblastoma and to explore their tumor source.

Mon Jan 03 00:00:00 EST 2022

Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer.

Cancer immunology research

Mujal AM,Combes AJ,Rao AA,Binnewies M,Samad B,Tsui J,Boissonnas A,Pollack JL,Argüello RJ,Meng MV,Porten SP,Ruhland MK,Barry KC,Chan V,Krummel MF

47-0459-42 was used in Flow cytometry/Cell sorting to show that holistic analysis of monocyte-to-macrophage differentiation creates a framework for critically different immune states.

Fri Apr 01 00:00:00 EDT 2022

Global absence and targeting of protective immune states in severe COVID-19.

Nature

Combes AJ,Courau T,Kuhn NF,Hu KH,Ray A,Chen WS,Chew NW,Cleary SJ,Kushnoor D,Reeder GC,Shen A,Tsui J,Hiam-Galvez KJ,Muñoz-Sandoval P,Zhu WS,Lee DS,Sun Y,You R,Magnen M,Rodriguez L,Im KW,Serwas NK,Leligdowicz A,Zamecnik CR,Loudermilk RP,Wilson MR,Ye CJ,Fragiadakis GK,Looney MR,Chan V,Ward A,Carrillo S,Matthay M,Erle DJ,Woodruff PG,Langelier C,Kangelaris K,Hendrickson CM,Calfee C,Rao AA,Krummel MF

47-0459-42 was used in Flow Cytometry to reveal potential targets for immunotherapies in patients with severe COVID-19 to re-engage viral defence.

Mon Mar 01 00:00:00 EST 2021

Dynamic contrast-enhanced magnetic resonance imaging quantification of leukemia-induced changes in bone marrow vascular function.

Haematologica

Gomes AL,Gribben J,Siow B,Passaro D,Bonnet D

47-0459-42 was used in Flow Cytometry to show that dynamic contrast enhanced (DCE) magnetic resonance imaging (MRI) can quantify the impact of human acute myeloid leukemia (AML) on bone marrow (BM) vasculature by optimizing an in vivo DCE-MRI method and assessing the method’s diagnostic and prognostic potential.

Sun Aug 01 00:00:00 EDT 2021

IgE-Based Therapeutic Combination Enhances Antitumor Response in Preclinical Models of Pancreatic Cancer.

Molecular cancer therapeutics

Markov SD,Caffrey TC,O'Connell KA,Grunkemeyer JA,Shin S,Hanson R,Patil PP,Shukla SK,Gonzalez D,Crawford AJ,Vance KE,Huang Y,Eberle KC,Radhakrishnan P,Grandgenett PM,Singh PK,Madiyalakan R,Daniels-Wells TR,Penichet ML,Nicodemus CF,Poole JA,Jaffee EM,Hollingsworth MA,Mehla K

47-0459-42 was used in Flow cytometry/Cell sorting to demonstrate the novel tumor protective benefits of tumor antigen-specific IgE-based therapeutics in a preclinical model of pancreatic cancer, which can open new avenues for future clinical interventions.

Wed Dec 01 00:00:00 EST 2021

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

47-0459 was used in Flow cytometry/Cell sorting to elucidate features of of human prenatal skin lymphocytes by using mass and flow cytometry.

Tue Nov 17 00:00:00 EST 2020

The Master Regulator Protein BAZ2B Can Reprogram Human Hematopoietic Lineage-Committed Progenitors into a Multipotent State.

Cell reports

Arumugam K,Shin W,Schiavone V,Vlahos L,Tu X,Carnevali D,Kesner J,Paull EO,Romo N,Subramaniam P,Worley J,Tan X,Califano A,Cosma MP

47-0459 was used in Flow cytometry/Cell sorting to identify the early driving events of human B cell reprogramming.

Tue Dec 08 00:00:00 EST 2020

Global Absence and Targeting of Protective Immune States in Severe COVID-19.

bioRxiv : the preprint server for biology

Combes AJ,Courau T,Kuhn NF,Hu KH,Ray A,Chen WS,Cleary SJ,Chew NW,Kushnoor D,Reeder GC,Shen A,Tsui J,Hiam-Galvez KJ,Muñoz-Sandoval P,Zhu WS,Lee DS,Sun Y,You R,Magnen M,Rodriguez L,Leligdowicz A,Zamecnik CR,Loudermilk RP,Wilson MR,Ye CJ,Fragiadakis GK,Looney MR,Chan V,Ward A,Carrillo S,Matthay M,Erle DJ,Woodruff PG,Langelier C,Kangelaris K,Hendrickson CM,Calfee C,Rao AA,Krummel MF

47-0459-42 was used in Flow Cytometry to gain an understanding of the severe/mild distinction in COVID-19 pathology, and its origins.

Thu Oct 29 00:00:00 EDT 2020

Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4⁺ T Cell Immunity.

Cell

Binnewies M,Mujal AM,Pollack JL,Combes AJ,Hardison EA,Barry KC,Tsui J,Ruhland MK,Kersten K,Abushawish MA,Spasic M,Giurintano JP,Chan V,Daud AI,Ha P,Ye CJ,Roberts EW,Krummel MF

47-0459 was used in Flow cytometry/Cell sorting to highlight a pathway that restrains cDC2 and whose reversal enhances CD4+ Tconv abundance and controls tumor growth.

Thu Apr 18 00:00:00 EDT 2019

A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments.

Nature medicine

Barry KC,Hsu J,Broz ML,Cueto FJ,Binnewies M,Combes AJ,Nelson AE,Loo K,Kumar R,Rosenblum MD,Alvarado MD,Wolf DM,Bogunovic D,Bhardwaj N,Daud AI,Ha PK,Ryan WR,Pollack JL,Samad B,Asthana S,Chan V,Krummel MF

47-0459 was used in Flow cytometry/Cell sorting to reveal that innate immune stimulatory dendritic cells and natural killer cells cluster together as an excellent prognostic tool for T cell-directed immunotherapy and that these innate cells are necessary for enhanced T cell tumor responses, suggesting this axis as a target for new therapies.

Wed Aug 01 00:00:00 EDT 2018

Characterization of a switchable chimeric antigen receptor platform in a pre-clinical solid tumor model.

Oncoimmunology

Pishali Bejestani E,Cartellieri M,Bergmann R,Ehninger A,Loff S,Kramer M,Spehr J,Dietrich A,Feldmann A,Albert S,Wermke M,Baumann M,Krause M,Bornhäuser M,Ehninger G,Bachmann M,von Bonin M

47-0459 was used in Flow cytometry/Cell sorting to illustrate that the UniCAR platform mediates potent anti-tumor activity in a relevant in vitro and in vivo solid tumor model.

Tue Jan 12 00:00:00 EST 2021

Characterization of CD33/CD3 Tetravalent Bispecific Tandem Diabodies (TandAbs) for the Treatment of Acute Myeloid Leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Reusch U,Harrington KH,Gudgeon CJ,Fucek I,Ellwanger K,Weichel M,Knackmuss SH,Zhukovsky EA,Fox JA,Kunkel LA,Guenot J,Walter RB

47-0459 was used in Flow cytometry/Cell sorting to demonstrate that several CD33/CD3 TandAbs could induce potent, dose-dependent cytolysis of CD33(+) AML cell lines.

Thu Dec 01 00:00:00 EST 2016

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

47-0459 was used in Flow cytometry/Cell sorting to showcase the therapeutic potential of gene-editing coupled with stem cell technology for the treatment of recessive dystrophic epidermolysis bullosa.

Wed Sep 30 00:00:00 EDT 2020

SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.

Nature communications

Mian SA,Rouault-Pierre K,Smith AE,Seidl T,Pizzitola I,Kizilors A,Kulasekararaj AG,Bonnet D,Mufti GJ

47-0459 was used in Flow cytometry/Cell sorting to demonstrate that mutations to SF3B1 can arise from CD34(+)CD38(-)CD45RA(-)CD90(+)CD49f(+) haematopoietic stem cells and is an initiating event in myelodysplastic syndrome patients.

Tue Dec 08 00:00:00 EST 2015

Reprogramming human endothelial cells to haematopoietic cells requires vascular induction.

Nature

Sandler VM,Lis R,Liu Y,Kedem A,James D,Elemento O,Butler JM,Scandura JM,Rafii S

47-0459 was used in Flow cytometry/Cell sorting to investigate the role of inductive cues from the vascular niche in coordinating and sustaining haematopoietic specification.

Thu Jul 17 00:00:00 EDT 2014

T cell-derived IL-22 amplifies IL-1β-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.

Diabetes

Dalmas E,Venteclef N,Caer C,Poitou C,Cremer I,Aron-Wisnewsky J,Lacroix-Desmazes S,Bayry J,Kaveri SV,Clément K,André S,Guerre-Millo M

47-0459 was used in Flow cytometry/Cell sorting to investigate the contribution of different cytokines to the proinflammatory signature of adipose tissue macrophages in type 2 diabetic obese patients.

Sun Jun 01 00:00:00 EDT 2014

Purification and direct transformation of epithelial progenitor cells from primary human prostate.

Nature protocols

Goldstein AS,Drake JM,Burnes DL,Finley DS,Zhang H,Reiter RE,Huang J,Witte ON

47-0459 was used in Flow cytometry/Cell sorting to describe a system to directly transform freshly isolated primary human prostate epithelial cells without previous culture or immortalisation.

Sun May 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (HI30), APC-eFluor™ 780, eBioscience™

CD45 Antibody (47-0459-42) in Flow

Product Details

47-0459-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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