Invitrogen

IL-6 Monoclonal Antibody (MP5-20F3), eFluor™ 450, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (1)
Published Figures (25)

FIGURE: 1 / 26

IL-6 Antibody (48-7061-82) in Flow

Mouse thioglycolate-elicited peritoneal exudate cells were either unstimulated (left) or stimulated with LPS in the presence of Brefeldin A (Product # 00-4506-51) overnight (right). The cells were surface stained with Anti-Mouse F4/80 Antigen eFluor® 660 (Product # 50-4801-82) and intracellularly stained with 0.25 µg of Anti-Mouse IL-6 eFluor® 450 using the Intracellular Fixation & Perbeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), ... View More

IL-6 Antibody in Immunocytochemistry (ICC/IF)

IL-6 Antibody in Immunohistochemistry (IHC)

IL-6 Antibody in Immunohistochemistry, ELISA (IHC, ELISA)

Product Image Gallery

Product Details

48-7061-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 6 publications 6 publications

ELISA (ELISA)

View 6 publications 6 publications

Neutralization (Neu)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP5-20F3

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574103

Product Specific Information

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported: This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MP5-20F3 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2023

A low-carbohydrate diet induces hepatic insulin resistance and metabolic associated fatty liver disease in mice.

Molecular metabolism

Long F,Bhatti MR,Kellenberger A,Sun W,Modica S,Höring M,Liebisch G,Krieger JP,Wolfrum C,Challa TD

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Neutralization

Wed Mar 01 00:00:00 EST 2023

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

48-7061-82 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunocytochemistry

Mon Feb 28 00:00:00 EST 2022

Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.

The Journal of experimental medicine

Rupert JE,Narasimhan A,Jengelley DHA,Jiang Y,Liu J,Au E,Silverman LM,Sandusky G,Bonetto A,Cao S,Lu X,O'Connell TM,Liu Y,Koniaris LG,Zimmers TA

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Neutralization

Mon Jun 07 00:00:00 EDT 2021

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE^-/- mice fed high fat diet.

Journal of biomedical science

Lee GL,Liao TL,Wu JY,Wu KK,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Mon Nov 08 00:00:00 EST 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Fri Jul 02 00:00:00 EDT 2021

Betulinic Acid Ameliorates the Severity of Acute Pancreatitis via Inhibition of the NF-κB Signaling Pathway in Mice.

International journal of molecular sciences

Zhou Z,Choi JW,Shin JY,Kim DU,Kweon B,Oh H,Kim YC,Song HJ,Bae GS,Park SJ

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunocytochemistry

Sat Jun 26 00:00:00 EDT 2021

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunohistochemistry

Wed Jul 01 00:00:00 EDT 2020

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Immunohistochemistry

Wed Jul 01 00:00:00 EDT 2020

Differential Activation of Macrophages Based on Their Environment in Advanced Age.

Chonnam medical journal

Lee DY,Lim JS,Cho KA

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Wed Jan 01 00:00:00 EST 2020

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Wed May 29 00:00:00 EDT 2019

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in ELISA

Wed Dec 04 00:00:00 EST 2019

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Sprouty1 induces a senescence-associated secretory phenotype by regulating NFκB activity: implications for tumorigenesis.

Cell death and differentiation

Macià A,Vaquero M,Gou-Fàbregas M,Castelblanco E,Valdivielso JM,Anerillas C,Mauricio D,Matias-Guiu X,Ribera J,Encinas M

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Neutralization

Sat Feb 01 00:00:00 EST 2014

IL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation.

International immunology

Wu Y,El Shikh ME,El Sayed RM,Best AM,Szakal AK,Tew JG

Published figure using IL-6 monoclonal antibody (Product # 48-7061-82) in Neutralization

Mon Jun 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

View more View less

Gene Aliases: Il-6; Il6

View more View less

UniProt ID: (Mouse) P08505

View more View less

Entrez Gene ID: (Mouse) 16193

View more View less

Function(s)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-6 Monoclonal Antibody (MP5-20F3), eFluor™ 450, eBioscience™

IL-6 Antibody (48-7061-82) in Flow

Product Details

48-7061-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!