Invitrogen

CD45 Monoclonal Antibody (2D1), eFluor™ 450, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

14 Published Figures

18 References

View all (406) CD45 antibodies

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CD45 Antibody (48-9459-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD45 eFluor® 450 (purple histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD45 Antibody — Antibody clone (2D1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 2D1 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (2D1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

48-9459-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D1

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1603240

Product Specific Information

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cryopreserved PM21-Particle-Expanded Natural Killer Cells Maintain Cytotoxicity and Effector Functions In Vitro and In Vivo.

Frontiers in immunology

Oyer JL,Croom-Perez TJ,Dieffenthaller TA,Robles-Carillo LD,Gitto SB,Altomare DA,Copik AJ

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Mon Aug 08 00:00:00 EDT 2022

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Defining the emergence of myeloid-derived suppressor cells in breast cancer using single-cell transcriptomics.

Science immunology

Alshetaiwi H,Pervolarakis N,McIntyre LL,Ma D,Nguyen Q,Rath JA,Nee K,Hernandez G,Evans K,Torosian L,Silva A,Walsh C,Kessenbrock K

48-9459 was used in Flow cytometry/Cell sorting to establish the MDSC-specific gene signature and identify CD84 as a surface marker for improved detection and enrichment of MDSCs in breast cancers.

Fri Feb 21 00:00:00 EST 2020

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Cell death & disease

Li X,Ma R,Gu Q,Liang L,Wang L,Zhang Y,Wang X,Liu X,Li Z,Fang J,Wu J,Wang Y,Li W,Hu B,Wang L,Zhou Q,Hao J

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Thu Aug 30 00:00:00 EDT 2018

Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.

Nature communications

Nguyen QH,Pervolarakis N,Blake K,Ma D,Davis RT,James N,Phung AT,Willey E,Kumar R,Jabart E,Driver I,Rock J,Goga A,Khan SA,Lawson DA,Werb Z,Kessenbrock K

48-9459 was used in Flow cytometry/Cell sorting to profile the transcriptomes of 25,790 primary human breast epithelial cells isolated from reduction mammoplasties, using single cell RNA sequencing.

Wed May 23 00:00:00 EDT 2018

Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease.

Nature communications

Moss J,Magenheim J,Neiman D,Zemmour H,Loyfer N,Korach A,Samet Y,Maoz M,Druid H,Arner P,Fu KY,Kiss E,Spalding KL,Landesberg G,Zick A,Grinshpun A,Shapiro AMJ,Grompe M,Wittenberg AD,Glaser B,Shemer R,Kaplan T,Dor Y

48-9459 was used in Flow cytometry/Cell sorting to develop a protocol for unbiased determination of the tissue origins of circulating cell-free DNA, using a reference methylation atlas of 25 human tissues and cell types.

Thu Nov 29 00:00:00 EST 2018

Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.

Obesity (Silver Spring, Md.)

Subramanian S,Pallati PK,Rai V,Sharma P,Agrawal DK,Nandipati KC

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Thu Nov 17 00:00:00 EST 2016

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

48-9459 was used in Flow cytometry/Cell sorting to demonstrate that endothelial Notch ligands promote PSC-definitive haematopoiesis and production of long-term engrafting CD34+ cells.

Mon Mar 02 00:00:00 EST 2015

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

48-9459 was used in Flow cytometry/Cell sorting to demonstrate that endothelial Notch ligands promote PSC-definitive haematopoiesis and production of long-term engrafting CD34+ cells.

Mon Mar 02 00:00:00 EST 2015

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD45 monoclonal antibody (Product # 48-9459-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45 Monoclonal Antibody (2D1), eFluor™ 450, eBioscience™

CD45 Antibody (48-9459-42) in Flow

Product Details

48-9459-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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