Invitrogen

CD3 Monoclonal Antibody (17A2), eFluor™ 660, eBioscience™

CD3 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 82

CD3 Antibody (50-0032-82) in IHC (F)

Immunohistochemistry of frozen mouse spleen stained with 10 µg/mL Anti-Mouse CD3 eFluor® 660 and costained with 10 µg/mL Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488. Nuclei are stained with DAPI (right).

CD3 Antibody in Immunohistochemistry (IHC)

CD3 Antibody in Immunocytochemistry (ICC/IF)

CD3 Antibody in Immunocytochemistry, Immunohistochemistry (Paraffin) (ICC/IF, IHC (P))

CD3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CD3 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

50-0032-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 17 publications 17 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

0.25 µg/test

View 62 publications 62 publications

T-Cell Activation (TCA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

17A2

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598657

Product Specific Information

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 17A2 antibody has also been testedy by immunohistochemistry on frozen mouse spleen and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

50-0032-82 was used in Flow cytometry/Cell sorting to show that platelet factors transfer the benefits of young blood to the ageing brain.

Tue Aug 01 00:00:00 EDT 2023

The activity of the aryl hydrocarbon receptor in T cells tunes the gut microenvironment to sustain autoimmunity and neuroinflammation.

PLoS biology

Merchak AR,Cahill HJ,Brown LC,Brown RM,Rivet-Noor C,Beiter RM,Slogar ER,Olgun DG,Gaultier A

50-0032-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to demonstrate a newly emerging role for AHR in mediating the interdependence between T lymphocytes and the microbiota, while simultaneously identifying new potential molecular targets for the treatment of MS and other autoimmune diseases.

Wed Feb 01 00:00:00 EST 2023

The activity of the aryl hydrocarbon receptor in T cells tunes the gut microenvironment to sustain autoimmunity and neuroinflammation.

PLoS biology

Merchak AR,Cahill HJ,Brown LC,Brown RM,Rivet-Noor C,Beiter RM,Slogar ER,Olgun DG,Gaultier A

Wed Feb 01 00:00:00 EST 2023

A single-cell atlas reveals the heterogeneity of meningeal immunity in a mouse model of Methyl CpG binding protein 2 deficiency.

Frontiers in immunology

Li H,Hu M,Huang Z,Wang Y,Xu Y,Deng J,Zhu M,Feng W,Xu X

50-0032-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to provide novel insights into meningeal immunity, which is a less studied aspect of neuroimmune interactions in Mecp2-mutated diseases, and offer an essential resource for comparative analyses and data exploration to better understand the functional role of meningeal immunity in RTT.

Tue Jan 31 00:00:00 EST 2023

A single-cell atlas reveals the heterogeneity of meningeal immunity in a mouse model of Methyl CpG binding protein 2 deficiency.

Frontiers in immunology

Li H,Hu M,Huang Z,Wang Y,Xu Y,Deng J,Zhu M,Feng W,Xu X

Tue Jan 31 00:00:00 EST 2023

LncRNA ANRIL-mediated miR-181b-5p/S1PR1 axis is involved in the progression of uremic cardiomyopathy through activating T cells.

Scientific reports

Xu Y,Cao L,Ji S,Shen W

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Oct 27 00:00:00 EDT 2022

Estrogen Protects against Renal Ischemia-Reperfusion Injury by Regulating Th17/Treg Cell Immune Balance.

Disease markers

Zhang Y,Chang Y,Han Z,Ma K,Zeng X,Li L

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Therapeutic CFTR Correction Normalizes Systemic and Lung-Specific S1P Level Alterations Associated with Heart Failure.

International journal of molecular sciences

Uhl FE,Vanherle L,Matthes F,Meissner A

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Jan 14 00:00:00 EST 2022

Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

The Journal of biological chemistry

Yan Q,Gao H,Yao Q,Ling K,Xiao G

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Choroid plexus-selective inactivation of adenosine A_2A receptors protects against T cell infiltration and experimental autoimmune encephalomyelitis.

Journal of neuroinflammation

Zheng W,Feng Y,Zeng Z,Ye M,Wang M,Liu X,Tang P,Shang H,Sun X,Lin X,Wang M,Li Z,Weng Y,Guo W,Vakal S,Chen JF

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Feb 18 00:00:00 EST 2022

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Intracellular aggregation of peptide-reprogrammed small molecule nanoassemblies enhances cancer chemotherapy and combinatorial immunotherapy.

Acta pharmaceutica Sinica. B

Peng J,Xiao Y,Yang Q,Liu Q,Chen Y,Shi K,Hao Y,Han R,Qian Z

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

PD-L1 overexpression conveys tolerance of mesenchymal stem cell-derived cardiomyocyte-like cells in an allogeneic mouse model.

Journal of cellular physiology

Mardomi A,Limoni SK,Rahbarghazi R,Mohammadi N,Khorashadizadeh M,Ranjbaran H,Nataj HH,Jafari N,Hasani B,Abediankenari S

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Wed Sep 01 00:00:00 EDT 2021

Early inflammation precedes cardiac fibrosis and heart failure in desmoglein 2 murine model of arrhythmogenic cardiomyopathy.

Cell and tissue research

Ng KE,Delaney PJ,Thenet D,Murtough S,Webb CM,Zaman N,Tsisanova E,Mastroianni G,Walker SLM,Westaby JD,Pennington DJ,Pink R,Kelsell DP,Tinker A

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Neuroimmune Consequences of eIF4E Phosphorylation on Chemotherapy-Induced Peripheral Neuropathy.

Frontiers in immunology

Agalave NM,Mody PH,Szabo-Pardi TA,Jeong HS,Burton MD

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Functional inhibition of lactate dehydrogenase suppresses pancreatic adenocarcinoma progression.

Clinical and translational medicine

Cheng CS,Tan HY,Wang N,Chen L,Meng Z,Chen Z,Feng Y

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry (Paraffin)

Tue Jun 01 00:00:00 EDT 2021

Functional inhibition of lactate dehydrogenase suppresses pancreatic adenocarcinoma progression.

Clinical and translational medicine

Cheng CS,Tan HY,Wang N,Chen L,Meng Z,Chen Z,Feng Y

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry (Paraffin)

Tue Jun 01 00:00:00 EDT 2021

Molecular Mechanisms Underpinning the Circulation and Cellular Uptake of Mycobacterium ulcerans Toxin Mycolactone.

Frontiers in pharmacology

Tello Rubio B,Bugault F,Baudon B,Raynal B,Brûlé S,Morel JD,Saint-Auret S,Blanchard N,Demangel C,Guenin-Macé L

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

Expression of CCRL2 Inhibits Tumor Growth by Concentrating Chemerin and Inhibiting Neoangiogenesis.

Cancers

Al Delbany D,Robert V,Dubois-Vedrenne I,Del Prete A,Vernimmen M,Radi A,Lefort A,Libert F,Wittamer V,Sozzani S,Parmentier M

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Tue Oct 05 00:00:00 EDT 2021

Small Molecule Potentiator of Adjuvant Activity Enhancing Survival to Influenza Viral Challenge.

Frontiers in immunology

Saito T,Sako Y,Sato-Kaneko F,Hosoya T,Yao S,Lao FS,Shpigelman J,Messer K,Pu M,Shukla NM,Chan M,Chu PJ,Cottam HB,Hayashi T,Carson DA,Corr M

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Dec 10 00:00:00 EST 2021

Gut Microbiota Dysbiosis Induced by Intracerebral Hemorrhage Aggravates Neuroinflammation in Mice.

Frontiers in microbiology

Yu X,Zhou G,Shao B,Zhou H,Xu C,Yan F,Wang L,Chen G,Li J,Fu X

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma.

Cell reports methods

Fitzgerald B,Connolly KA,Cui C,Fagerberg E,Mariuzza DL,Hornick NI,Foster GG,William I,Cheung JF,Joshi NS

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunocytochemistry

Mon Sep 27 00:00:00 EDT 2021

Inducible T-Cell Costimulator Mediates Lymphocyte/Macrophage Interactions During Liver Repair.

Frontiers in immunology

Ramavath NN,Gadipudi LL,Provera A,Gigliotti LC,Boggio E,Bozzola C,Albano E,Dianzani U,Sutti S

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Feb 14 00:00:00 EST 2022

Soft implantable drug delivery device integrated wirelessly with wearable devices to treat fatal seizures.

Science advances

Joo H,Lee Y,Kim J,Yoo JS,Yoo S,Kim S,Arya AK,Kim S,Choi SH,Lu N,Lee HS,Kim S,Lee ST,Kim DH

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

50-0032 was used in Flow cytometry/Cell sorting to uncover a mechanism that controls myeloid regeneration and early lineage decisions in HSCs and could be targeted in LSCs to normalize leukemic myeloid cell production.

Mon Mar 02 00:00:00 EST 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Mon Mar 02 00:00:00 EST 2020

Modulation of PKM activity affects the differentiation of T_H17 cells.

Science signaling

Seki SM,Posyniak K,McCloud R,Rosen DA,Fernández-Castañeda A,Beiter RM,Serbulea V,Nanziri SC,Hayes N,Spivey C,Gemta L,Bullock TNJ,Hsu KL,Gaultier A

50-0032 was used in Immunohistochemistry to assessed TEPP-46 and DASA-58 therapeutic activity in a mouse model of T cell-mediated autoimmunity that mimics multiple sclerosis (MS).

Tue Oct 27 00:00:00 EDT 2020

Novel enhanced GFP-positive congenic inbred strain establishment and application of tumor-bearing nude mouse model.

Cancer science

Lan Q,Chen Y,Dai C,Li S,Fei X,Dong J,Shen Y,Dai X,Lu Z,Liu B,Wang Q,Wang H,Zhou Z,Ji X,Wang Z,Huang Q

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

In Situ Therapeutic Cancer Vaccination with an Oncolytic Virus Expressing Membrane-Tethered IL-2.

Molecular therapy oncolytics

Liu W,Dai E,Liu Z,Ma C,Guo ZS,Bartlett DL

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

Loss of mural cell-derived laminin aggravates hemorrhagic brain injury.

Journal of neuroinflammation

Gautam J,Xu L,Nirwane A,Nguyen B,Yao Y

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Mon Apr 06 00:00:00 EDT 2020

Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Stroma remodeling and reduced cell division define durable response to PD-1 blockade in melanoma.

Nature communications

Galvani E,Mundra PA,Valpione S,Garcia-Martinez P,Smith M,Greenall J,Thakur R,Helmink B,Andrews MC,Boon L,Chester C,Gremel G,Hogan K,Mandal A,Zeng K,Banyard A,Ashton G,Cook M,Lorigan P,Wargo JA,Dhomen N,Marais R

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry (Frozen)

Wed Feb 12 00:00:00 EST 2020

Stroma remodeling and reduced cell division define durable response to PD-1 blockade in melanoma.

Nature communications

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry (Frozen)

Wed Feb 12 00:00:00 EST 2020

Triptolide Attenuates Transplant Vasculopathy Through Multiple Pathways.

Frontiers in immunology

Luo Z,Liao T,Zhang Y,Zheng H,Sun Q,Han F,Yang Z,Sun Q

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Mar 25 00:00:00 EDT 2021

Developmental Dysfunction of the Central Nervous System Lymphatics Modulates the Adaptive Neuro-Immune Response in the Perilesional Cortex in a Mouse Model of Traumatic Brain Injury.

Frontiers in immunology

Wojciechowski S,Virenque A,Vihma M,Galbardi B,Rooney EJ,Keuters MH,Antila S,Koistinaho J,Noe FM

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry (Frozen)

Wed May 19 00:00:00 EDT 2021

Cooperation between Constitutive and Inducible Chemokines Enables T Cell Engraftment and Immune Attack in Solid Tumors.

Cancer cell

Dangaj D,Bruand M,Grimm AJ,Ronet C,Barras D,Duttagupta PA,Lanitis E,Duraiswamy J,Tanyi JL,Benencia F,Conejo-Garcia J,Ramay HR,Montone KT,Powell DJ,Gimotty PA,Facciabene A,Jackson DG,Weber JS,Rodig SJ,Hodi SF,Kandalaft LE,Irving M,Zhang L,Foukas P,Rusakiewicz S,Delorenzi M,Coukos G

50-0032-82 was used in Flow Cytometry to investigate the role of chemokines in regulating T cell accumulation in solid tumors.

Mon Jun 10 00:00:00 EDT 2019

TNF-α Coordinates Hematopoietic Stem Cell Survival and Myeloid Regeneration.

Cell stem cell

Yamashita M,Passegué E

50-0032 was used in Flow cytometry/Cell sorting to reveal several TNF-α-mediated pro-survival mechanisms unique to HSCs, highlight an important role for necroptosis in HSC killing, and establish TNF-α as a major pro-survival and pro-regeneration factor for HSCs.

Thu Sep 05 00:00:00 EDT 2019

Deletion of C-C Motif Chemokine Ligand 5 Worsens Invariant Natural Killer T-Cell-Mediated Hepatitis via Compensatory Up-regulation of CXCR2-Related Chemokine Activity.

Cellular and molecular gastroenterology and hepatology

Chen L,Gu J,Qian Y,Li M,Qian Y,Xu M,Li J,Wen Y,Xia L,Li J,Xia Q,Kong X,Wu H

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Jun 04 00:00:00 EDT 2019

Blood autophagy defect causes accelerated non-hematopoietic organ aging.

Aging

Fang Y,Zhu L,An N,Jiang G,Qian J,Zhao R,Yuan N,Zhang S,Wang J

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Sun Jul 21 00:00:00 EDT 2019

MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment.

Journal for immunotherapy of cancer

Fang DD,Tang Q,Kong Y,Wang Q,Gu J,Fang X,Zou P,Rong T,Wang J,Yang D,Zhai Y

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Nature communications

Pascual-García M,Bonfill-Teixidor E,Planas-Rigol E,Rubio-Perez C,Iurlaro R,Arias A,Cuartas I,Sala-Hojman A,Escudero L,Martínez-Ricarte F,Huber-Ruano I,Nuciforo P,Pedrosa L,Marques C,Braña I,Garralda E,Vieito M,Squatrito M,Pineda E,Graus F,Espejo C,Sahuquillo J,Tabernero J,Seoane J

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Persistence of Integrase-Deficient Lentiviral Vectors Correlates with the Induction of STING-Independent CD8⁺ T Cell Responses.

Cell reports

Cousin C,Oberkampf M,Felix T,Rosenbaum P,Weil R,Fabrega S,Morante V,Negri D,Cara A,Dadaglio G,Leclerc C

50-0032 was used in Flow cytometry/Cell sorting to demonstrate that integrase-deficient lentiviral vectors can trigger persistent adaptive response by the mediation of a weak and transient innate response alongside the persistence of the vector in tissues.

Tue Jan 29 00:00:00 EST 2019

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Thu Jun 28 00:00:00 EDT 2018

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Thu Jun 28 00:00:00 EDT 2018

The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.

Cell research

Chen J,Cai Z,Bai M,Yu X,Zhang C,Cao C,Hu X,Wang L,Su R,Wang D,Wang L,Yao Y,Ye R,Hou B,Yu Y,Yu S,Li J,Xue Y

50-0032 was used in Immunohistochemistry-immunofluorescence to identify an RNA-binding protein that is both required and sufficient to define activation-induced cytidine deaminase-binding sites genome-wide in activated B cells.

Mon Oct 01 00:00:00 EDT 2018

Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.

The Journal of clinical investigation

Bahmani B,Uehara M,Jiang L,Ordikhani F,Banouni N,Ichimura T,Solhjou Z,Furtmüller GJ,Brandacher G,Alvarez D,von Andrian UH,Uchimura K,Xu Q,Vohra I,Yilmam OA,Haik Y,Azzi J,Kasinath V,Bromberg JS,McGrath MM,Abdi R

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Western Blot

Thu Nov 01 00:00:00 EDT 2018

Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.

The Journal of clinical investigation

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Western Blot

Thu Nov 01 00:00:00 EDT 2018

Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.

The Journal of clinical investigation

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Western Blot

Thu Nov 01 00:00:00 EDT 2018

Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells.

World journal of gastroenterology

Müller-Graff FT,Fitzner B,Jaster R,Vollmar B,Zechner D

50-0032 was used in Flow cytometry/Cell sorting to evaluate the influence of hyperglycaemia on the progression of autoimmune pancreatitis.

Sat Jul 28 00:00:00 EDT 2018

Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells.

World journal of gastroenterology

Müller-Graff FT,Fitzner B,Jaster R,Vollmar B,Zechner D

50-0032 was used in Flow cytometry/Cell sorting to evaluate the influence of hyperglycaemia on the progression of autoimmune pancreatitis.

Sat Jul 28 00:00:00 EDT 2018

Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells.

World journal of gastroenterology

Müller-Graff FT,Fitzner B,Jaster R,Vollmar B,Zechner D

50-0032 was used in Flow cytometry/Cell sorting to evaluate the influence of hyperglycaemia on the progression of autoimmune pancreatitis.

Sat Jul 28 00:00:00 EDT 2018

Neonatal adoptive transfer of lymphocytes rescues social behaviour during adolescence in immune-deficient mice.

The European journal of neuroscience

Clark SM,Vaughn CN,Soroka JA,Li X,Tonelli LH

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

CD4⁺ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.

Nature communications

García Nores GD,Ly CL,Cuzzone DA,Kataru RP,Hespe GE,Torrisi JS,Huang JJ,Gardenier JC,Savetsky IL,Nitti MD,Yu JZ,Rehal S,Mehrara BJ

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu May 17 00:00:00 EDT 2018

Microglia prevent peripheral immune cell invasion and promote an anti-inflammatory environment in the brain of APP-PS1 transgenic mice.

Journal of neuroinflammation

Unger MS,Schernthaner P,Marschallinger J,Mrowetz H,Aigner L

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Sep 21 00:00:00 EDT 2018

Microbiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice.

mSphere

Quach D,Collins F,Parameswaran N,McCabe L,Britton RA

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

Characterization of meningeal type 2 innate lymphocytes and their response to CNS injury.

The Journal of experimental medicine

Gadani SP,Smirnov I,Wiltbank AT,Overall CC,Kipnis J

Wed Feb 01 00:00:00 EST 2017

Diet-induced obesity causes visceral, but not subcutaneous, lymph node hyperplasia via increases in specific immune cell populations.

Cell proliferation

Magnuson AM,Regan DP,Fouts JK,Booth AD,Dow SW,Foster MT

50-0032 was used in Immunocytochemistry-immunoflourescence to investigate whether secondary lymphatics within visceral adiposity contribute to disease risk.

Sun Oct 01 00:00:00 EDT 2017

Silybin attenuates LPS-induced lung injury in mice by inhibiting NF-κB signaling and NLRP3 activation.

International journal of molecular medicine

Zhang B,Wang B,Cao S,Wang Y,Wu D

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Effect of Early-life Gut Mucosal Compromise on Disease Progression in NOD Mice.

Comparative medicine

Bendtsen KM,Hansen CH,Krych L,Buschard K,Farlov H,Hansen AK

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Recipient bone marrow assimilates the myeloid/lymphoid reconstitution of distinct fetal hematopoietic stem cells.

Oncotarget

Guo XL,Chu L,Ke F,Mu LL,Li Z,Cai JJ,Li HF,Hong DL

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Dec 12 00:00:00 EST 2017

Stimulation of hair follicle stem cell proliferation through an IL-1 dependent activation of γδT-cells.

eLife

Lee P,Gund R,Dutta A,Pincha N,Rana I,Ghosh S,Witherden D,Kandyba E,MacLeod A,Kobielak K,Havran WL,Jamora C

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in T-Cell Activation

Mon Dec 04 00:00:00 EST 2017

Loss of epidermal AP1 transcription factor function reduces filaggrin level, alters chemokine expression and produces an ichthyosis-related phenotype.

Cell death & disease

Young CA,Rorke EA,Adhikary G,Xu W,Eckert RL

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

Rottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies.

PloS one

Min M,Yan BX,Wang P,Landeck L,Chen JQ,Li W,Cai SQ,Zheng M,Man XY

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Jan 16 00:00:00 EST 2018

Antibody-dependent fragmentation is a newly identified mechanism of cell killing in vivo.

Scientific reports

Liew PX,Kim JH,Lee WY,Kubes P

50-0032 was used in Flow cytometry/Cell sorting to investigate a novel antibody-dependent cell death pathway.

Tue Sep 05 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Laminin regulates PDGFRβ(+) cell stemness and muscle development.

Nature communications

Yao Y,Norris EH,Mason CE,Strickland S

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunofluorescence

Tue May 03 00:00:00 EDT 2016

CD8(+) T-cell Immune Evasion Enables Oncolytic Virus Immunotherapy.

EBioMedicine

Pourchet A,Fuhrmann SR,Pilones KA,Demaria S,Frey AB,Mulvey M,Mohr I

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

TNF-Mediated Restriction of Arginase 1 Expression in Myeloid Cells Triggers Type 2 NO Synthase Activity at the Site of Infection.

Cell reports

Schleicher U,Paduch K,Debus A,Obermeyer S,König T,Kling JC,Ribechini E,Dudziak D,Mougiakakos D,Murray PJ,Ostuni R,Körner H,Bogdan C

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue May 03 00:00:00 EDT 2016

Diet-dependent, microbiota-independent regulation of IL-10-producing lamina propria macrophages in the small intestine.

Scientific reports

Ochi T,Feng Y,Kitamoto S,Nagao-Kitamoto H,Kuffa P,Atarashi K,Honda K,Teitelbaum DH,Kamada N

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed Jun 15 00:00:00 EDT 2016

High salt reduces the activation of IL-4- and IL-13-stimulated macrophages.

The Journal of clinical investigation

Binger KJ,Gebhardt M,Heinig M,Rintisch C,Schroeder A,Neuhofer W,Hilgers K,Manzel A,Schwartz C,Kleinewietfeld M,Voelkl J,Schatz V,Linker RA,Lang F,Voehringer D,Wright MD,Hubner N,Dechend R,Jantsch J,Titze J,Müller DN

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

IKKβ acts as a tumor suppressor in cancer-associated fibroblasts during intestinal tumorigenesis.

The Journal of experimental medicine

Pallangyo CK,Ziegler PK,Greten FR

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Dec 14 00:00:00 EST 2015

Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses.

Human vaccines & immunotherapeutics

Rose AH,Hoffmann FW,Hara JH,Urschitz J,Moisyadi S,Hoffmann PR,Bertino P

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon May 16 00:00:00 EDT 2016

IL-33 Enhances Humoral Immunity Against Chronic HBV Infection Through Activating CD4(+)CXCR5(+) TFH Cells.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Zhao PW,Shi X,Li C,Ayana DA,Niu JQ,Feng JY,Wang J,Jiang YF

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.

Nature communications

Kang YJ,Bang BR,Han KH,Hong L,Shim EJ,Ma J,Lerner RA,Otsuka M

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Fri Sep 18 00:00:00 EDT 2015

Antibiotics in neonatal life increase murine susceptibility to experimental psoriasis.

Nature communications

Zanvit P,Konkel JE,Jiao X,Kasagi S,Zhang D,Wu R,Chia C,Ajami NJ,Smith DP,Petrosino JF,Abbatiello B,Nakatsukasa H,Chen Q,Belkaid Y,Chen ZJ,Chen W

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2015

Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality.

Nature communications

Alvarez S,Díaz M,Flach J,Rodriguez-Acebes S,López-Contreras AJ,Martínez D,Cañamero M,Fernández-Capetillo O,Isern J,Passegué E,Méndez J

50-0032 was used in Flow cytometry/Cell sorting to determine that haematopoietic progenitors are particularly sensitive to replication stress.

Mon Oct 12 00:00:00 EDT 2015

Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

Nature

Flach J,Bakker ST,Mohrin M,Conroy PC,Pietras EM,Reynaud D,Alvarez S,Diolaiti ME,Ugarte F,Forsberg EC,Le Beau MM,Stohr BA,Méndez J,Morrison CG,Passegué E

50-0032 was used in Flow cytometry/Cell sorting to study functional decline in ageing haematopoietic stem cells due to replicative stress.

Thu Aug 14 00:00:00 EDT 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

50-0032 was used in Flow cytometry/Cell sorting to show that conditional WASH deletion in the haematopoietic system causes defective blood production of the host.

Mon Sep 22 00:00:00 EDT 2014

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

50-0032 was used in Immunofluorescence on frozen tissues to investigate the role of MHC-II in lymph node stromal cells, showing that stromal cells constrain immunity through MHC-II expression.

Wed Nov 19 00:00:00 EST 2014

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

50-0032 was used in Immunofluorescence on frozen tissues to investigate the role of MHC-II in lymph node stromal cells, showing that stromal cells constrain immunity through MHC-II expression.

Wed Nov 19 00:00:00 EST 2014

A chitin-like component on sclerotic cells of Fonsecaea pedrosoi inhibits Dectin-1-mediated murine Th17 development by masking β-glucans.

PloS one

Dong B,Li D,Li R,Chen SC,Liu W,Liu W,Chen L,Chen Y,Zhang X,Tong Z,Xia Y,Xia P,Wang Y,Duan Y

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2015

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

Colonic patch and colonic SILT development are independent and differentially regulated events.

Mucosal immunology

Baptista AP,Olivier BJ,Goverse G,Greuter M,Knippenberg M,Kusser K,Domingues RG,Veiga-Fernandes H,Luster AD,Lugering A,Randall TD,Cupedo T,Mebius RE

50-0032 was used in Flow cytometry/Cell sorting to distinguish the different mechanisms responsible for the formation of two distinct colonic lymphoid tissues.

Wed May 01 00:00:00 EDT 2013

Neuronal and nonneuronal cholinergic structures in the mouse gastrointestinal tract and spleen.

The Journal of comparative neurology

Gautron L,Rutkowski JM,Burton MD,Wei W,Wan Y,Elmquist JK

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunofluorescence

Fri Nov 01 00:00:00 EDT 2013

Increased incidence of endometrioid tumors caused by aberrations in E-cadherin promoter of mismatch repair-deficient mice.

Carcinogenesis

Kovtun IV,Harris KJ,Jatoi A,Jevremovic D

Published figure using CD3 monoclonal antibody (Product # 50-0032-82) in Immunohistochemistry

Fri Jul 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

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UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

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Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (17A2), eFluor™ 660, eBioscience™

CD3 Antibody (50-0032-82) in IHC (F)

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50-0032-82

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