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Vimentin Antibody (757-9897-82) in IHC (P)
Immunohistochemical analysis of Vimentin was performed on FFPE human tonsil tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 10 mins, followed by a 5-min cool down and a 5-min wash with ddH2O. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30 mins and blocked with 3% BSA/5% normal goat serum/1X PBS for 1 ... View More
Product Details
757-9897-82
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
V9
Conjugate
Excitation/Emission Max
760/788 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer
Contains
0.05% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.
This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.
Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).
Applications Tested: This V9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 1.33 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis luminal protein 113; FLJ36605; RP11-124N14.1; VIME; Vimentin
Gene Aliases: CTRCT30; HEL113; VIM
UniProt ID: (Human) P08670
Entrez Gene ID: (Human) 7431
movement of cell or subcellular component
positive regulation of gene expression
negative regulation of neuron projection development
astrocyte development
viral process
muscle filament sliding
intermediate filament organization
Bergmann glial cell differentiation
SMAD protein signal transduction
lens fiber cell development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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