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APIP Antibody (MA1-41247) in IHC (P)
Immunohistochemical analysis of APIP in formalin-fixed, paraffin-embedded adenocarcinoma of the breast. Samples were incubated in APIP monoclonal antibody (Product # MA1-41247) using a dilution of 5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT ... View More
Product Details
MA1-41247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
19F461
Immunogen
Recombinant APIP protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1070436
Product Specific Information
Suggested positive control: HeLa or 293, antigen standard for APIP (transient overexpression lysate).
Target Information
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf 1 (for apoptosis protease-activating factor 1). Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), which leads to Caspase-9 activation. Activated Caspase-9 in turn cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, Cho et al (4) have identified a new Apaf 1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAF1-interacting protein; APIP; CG129; dJ179L10.2; hAPIP; Methylthioribulose-1-phosphate dehydratase; monocyte macrophage 19; Monocyte/macrophage protein 19; MTRu-1-P dehydratase; probable methylthioribulose-1-phosphate dehydratase
Gene Aliases: APIP; APIP2; CGI-29; CGI29; hAPIP; MMRP19
UniProt ID: (Human) Q96GX9, (Mouse) Q9WVQ5
Entrez Gene ID: (Human) 51074, (Mouse) 56369
apoptotic process
L-methionine biosynthetic process from S-adenosylmethionine
L-methionine biosynthetic process from methylthioadenosine
negative regulation of apoptotic process
protein homotetramerization
pyroptosis
regulation of ERK1 and ERK2 cascade
cellular amino acid biosynthetic process
methionine biosynthetic process
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