Invitrogen

ATF4 Monoclonal Antibody ([N360A/24), PerCP

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-45649

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

[N360A/24

Immunogen

Fusion protein amino acids 25-327 of human ATF4.
View immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932103

Product Specific Information

86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.

1 µg/mL of MA5-45649 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 60kDa. Does not cross-react with ATF5.

This antibody was formerly sold as clone S360A-24.

Target Information

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); activating transcription factor ATF-4; ATF 4; ATF4 protein; atf4 {ECO:0000312|RGD:621863}; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; OTTHUMP00000199130; rATF-4; tax-re; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67

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Gene Aliases: ATF4; CREB-2; CREB2; TAXREB67; TXREB

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UniProt ID: (Human) P18848, (Rat) Q9ES19

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Entrez Gene ID: (Human) 468, (Rat) 79255

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding leucine zipper domain binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity transcription factor binding DNA-binding transcription factor
Process(es)
gluconeogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cellular amino acid metabolic process gamma-aminobutyric acid signaling pathway positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of translational initiation in response to stress circadian regulation of gene expression cellular response to amino acid starvation cellular response to UV response to endoplasmic reticulum stress positive regulation of transcription from RNA polymerase II promoter in response to stress positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress PERK-mediated unfolded protein response cellular response to glucose starvation mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process negative regulation of potassium ion transport positive regulation of neuron apoptotic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase I promoter positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress negative regulation of oxidative stress-induced neuron death positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress response to manganese-induced endoplasmic reticulum stress transcription, DNA-templated regulation of transcription from RNA polymerase II promoter
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