Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
BACH1 Antibody (MA5-51374) in WB
Western blot analysis of BACH1 in various lysates. Samples were incubated with BACH1 Recombinant monoclonal antibody (Product # MA5-51374) using a dilution of 1:2,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:200,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-51374
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8A8X1
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 410-736 of human BACH1 (NP_001177.1).
Conjugate
Form
Liquid
Concentration
0.07 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3093683
Product Specific Information
Immunogen sequence: TPCQMQLSPA VAKDGSEQIS QKRSECPWLG IRISESPEPG QRTFTTLSSV NCPFISTLST EGCSSNLEIG NDDYVSEPQQ EPCPYACVIS LGDDSETDTE GDSESCSARE QECEVKLPFN AQRIISLSRN DFQSLLKMHK LTPEQLDCIH DIRRRSKNRI AAQRCRKRKL DCIQNLESEI EKLQSEKESL LKERDHILST LGETKQNLTG LCQKVCKEAA LSQEQIQILA KYSAADCPLS FLISEKDKST PDGELALPSI FSLSDRPPAV LPPCARGNSE PGYARGQESQ QMSTATSEQA GPAEQCRQSG GISDFCQQMT DKCTTDE
Target Information
BACH1.3 is a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo and/or hetero oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. Some exons of this gene overlap with some exons from the C21orf41 gene, which is transcribed in an opposite orientation to this gene, but which does not seem to encode a protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: basic region leucine zipper transcriptional regulator BACH1; BTB and CNC homolog 1; BTB and CNC homology 1 transcription factor; BTB and CNC homology 1, basic leucine zipper transcription factor 1; HA2303; Transcription regulator protein BACH1
Gene Aliases: 6230421P05Rik; AI323795; BACH-1; BACH1; BTBD24
UniProt ID: (Human) O14867, (Mouse) P97302
Entrez Gene ID: (Human) 571, (Mouse) 12013
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription factor activity, sequence-specific DNA binding
ubiquitin-protein transferase activity
protein binding
heme binding
DNA binding
sequence-specific DNA binding
regulation of transcription involved in G1/S transition of mitotic cell cycle
regulation of transcription involved in G2/M transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to hypoxia
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support