Proteintech

alpha Tubulin Monoclonal Antibody (1E4C11), CoraLite® 594

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Antibody Testing Data (5)

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

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alpha Tubulin Antibody (CL594-66031) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated version of this antibody, (Product # CL594-66031) (alpha Tubulin antibody), at dilution of 1:100.

alpha Tubulin Antibody in Western Blot (WB)

alpha Tubulin Antibody in Flow Cytometry (Flow)

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Product Details

CL594-66031

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1E4C11

Immunogen

Full length alpha Tubulin protein.

Conjugate

CoraLite® 594 CoraLite® 594 CoraLite® 594

Excitation/Emission Max

593/614 nm

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 0.5% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, store in dark

Shipping conditions

Wet ice

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Effects of IGF-1 on the Three-Dimensional Culture of Ovarian Preantral Follicles and Superovulation Rates in Mice.

Biology

Dai S,Zhang H,Yang F,Shang W,Zeng S

Sun May 29 00:00:00 EDT 2022

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes.

Experimental & molecular medicine

Wu LF,Zhang Q,Mo XB,Lin J,Wu YL,Lu X,He P,Wu J,Guo YF,Wang MJ,Ren WY,Deng HW,Lei SF,Deng FY

Tue Mar 01 00:00:00 EST 2022

Overexpression of Tfap2a in Mouse Oocytes Impaired Spindle and Chromosome Organization.

International journal of molecular sciences

Lin J,Ji Z,Di Z,Zhang Y,Yan C,Zeng S

Sat Nov 19 00:00:00 EST 2022

Bioinformatics

Protein Aliases: alpha tubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B