Invitrogen

Calreticulin Polyclonal Antibody

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Antibody Testing Data (3)
Published Figures (7)

Calreticulin Antibody in Western Blot (WB)

FIGURE: 1 / 10

Calreticulin Antibody (PA1-903) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), Jurkat (Lane 5) and A549 (Lane 6). The blot was probed with Chicken Anti-Calreticulin Polyclonal Antibody (Product # PA1-903, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to Calreticulin was observed across the cell lines tested. Known quantity... View More

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Product Details

PA1-903

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rabbit, Rat

Published species

Amphibian, Human, Mouse, Rabbit, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2228457

Product Specific Information

PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues.

PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract.

PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments.

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Insulin Treatment Reduces Susceptibility to Atrial Fibrillation in Type 1 Diabetic Mice.

Frontiers in cardiovascular medicine

Maria Z,Campolo AR,Scherlag BJ,Ritchey JW,Lacombe VA

PA1-903 was used in Western Blotting to suggest that insulin and glucose disturbances are sufficient to induce atrial fibrillation (AF) susceptibility during mild diabetes.

Mon Sep 28 00:00:00 EDT 2020

Insulin Treatment Reduces Susceptibility to Atrial Fibrillation in Type 1 Diabetic Mice.

Frontiers in cardiovascular medicine

Maria Z,Campolo AR,Scherlag BJ,Ritchey JW,Lacombe VA

PA1-903 was used in Western Blotting to suggest that insulin and glucose disturbances are sufficient to induce atrial fibrillation (AF) susceptibility during mild diabetes.

Mon Sep 28 00:00:00 EDT 2020

Glial Growth Factor 2 Regulates Glucose Transport in Healthy Cardiac Myocytes and During Myocardial Infarction via an Akt-Dependent Pathway.

Frontiers in physiology

Shoop S,Maria Z,Campolo A,Rashdan N,Martin D,Lovern P,Lacombe VA

PA1-903 was used in Western Blotting to investigate whether treatment with a version of glial growth factor 2 will enhance glucose transport in the healthy myocardium and during myocardial infarction.

Wed Sep 30 00:00:00 EDT 2020

Diabetes Alters the Expression and Translocation of the Insulin-Sensitive Glucose Transporters 4 and 8 in the Atria.

PloS one

Maria Z,Campolo AR,Lacombe VA

Published figure using Calreticulin polyclonal antibody (Product # PA1-903) in Western Blot

Tue Jun 28 00:00:00 EDT 2016

Gestational exposure to diethylstilbestrol alters cardiac structure/function, protein expression and DNA methylation in adult male mice progeny.

Toxicology and applied pharmacology

Haddad R,Kasneci A,Mepham K,Sebag IA,Chalifour LE

PA1-903 was used in western blot to study the effects of diethylstilbestrol exposure during gestation on epigenetic DNA modifications and cardiac structure and function in adult mice

Tue Jan 01 00:00:00 EST 2013

Bilateral acute pyogenic conjunctivitis with iritis induced by unilateral topical application of bacterial peptidoglycan muramyl dipeptide in adult rabbits.

Experimental eye research

Langford MP,Foreman BD,Srur L,Ganley JP,Redens TB

PA1-903 was used in immunohistochemistry to study the role of bacterial peptidoglycan muramyl dipeptide in the pathogenesis of acute pyogenic conjunctivitis and iritis

Fri Nov 01 00:00:00 EDT 2013

mTOR-Controlled Autophagy Requires Intracellular Ca(2+) Signaling.

PloS one

Decuypere JP,Kindt D,Luyten T,Welkenhuyzen K,Missiaen L,De Smedt H,Bultynck G,Parys JB

PA1-903 was used in western blot to study the role of intracellular Ca(2+) signaling in mTOR-modulated autophagy

Mon Sep 23 00:00:00 EDT 2013

Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and autophagy induction are interrelated.

Autophagy

Decuypere JP,Welkenhuyzen K,Luyten T,Ponsaerts R,Dewaele M,Molgó J,Agostinis P,Missiaen L,De Smedt H,Parys JB,Bultynck G

PA1-903 was used in western blot to study the relationship between Ins(1,4,5)P3 receptor-mediated Ca2+ signaling and the induction of autophagy

Thu Dec 01 00:00:00 EST 2011

Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.

Molecular & cellular proteomics : MCP

Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A

PA1-903 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse

Sat Mar 01 00:00:00 EST 2008

Cardiac mitochondrial connexin 43 regulates apoptosis.

Biochemical and biophysical research communications

Goubaeva F,Mikami M,Giardina S,Ding B,Abe J,Yang J

PA1-903 was used in western blot to investigate the role of cardiac mitochondrial connexin 43 in apoptosis

Fri Jan 05 00:00:00 EST 2007

Localization of the iron transport proteins Mobilferrin and DMT-1 in the duodenum: the surprising role of mucin.

American journal of hematology

Simovich M,Hainsworth LN,Fields PA,Umbreit JN,Conrad ME

PA1-903 was used in western blot to study the intestinal iron uptake and the involvement of mucin

Mon Sep 01 00:00:00 EDT 2003

C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells.

The Journal of experimental medicine

Ogden CA,deCathelineau A,Hoffmann PR,Bratton D,Ghebrehiwet B,Fadok VA,Henson PM

PA1-903 was used in immunocytochemistry to study the mechanisms of macropinocytosis and apoptotic cell removal

Mon Sep 17 00:00:00 EDT 2001

Association of ERp57 with mouse MHC class I molecules is tapasin dependent and mimics that of calreticulin and not calnexin.

Journal of immunology (Baltimore, Md. : 1950)

Harris MR,Lybarger L,Yu YY,Myers NB,Hansen TH

PA1-903 was used in western blot to study the interaction between ERp57 and mouse MHC class I molecules.

Fri Jun 01 00:00:00 EDT 2001

Interactions of HLA-B27 with the peptide loading complex as revealed by heavy chain mutations.

International immunology

Harris MR,Lybarger L,Myers NB,Hilbert C,Solheim JC,Hansen TH,Yu YY

PA1-903 was used in western blot to investigate how HLA-B27 interact with the peptide loading complex

Mon Oct 01 00:00:00 EDT 2001

Calreticulin inhibits repetitive intracellular Ca2+ waves.

Cell

Camacho P,Lechleiter JD

PA1-903 was used in immunocytochemistry to investigate the role of CRT in IP3-mediated caclium signaling

Fri Sep 08 00:00:00 EDT 1995

Chaperone function of calreticulin when expressed in the endoplasmic reticulum as the membrane-anchored and soluble forms.

The Journal of biological chemistry

Wada I,Imai S,Kai M,Sakane F,Kanoh H

PA1-903 was used in western blot to demonstrate that calreticulin in ER is another novel CAS-sensitive chaperone

Fri Sep 01 00:00:00 EDT 1995

Bioinformatics

Protein Aliases: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; calreticulin precursor; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)

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Gene Aliases: CALR; Calregulin; cC1qR; CRP55; CRT; CRTC; ERp60; HACBP; HEL-S-99n; I79_008346; RO; SSA

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UniProt ID: (Human) P27797, (Rabbit) P15253, (Chinese hamster) Q8K3H7, (Mouse) P14211, (Rat) P18418

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Entrez Gene ID: (Rabbit) 100350417, (Human) 811, (Rabbit) 100009050, (Dog) 476694, (Chinese hamster) 100689096, (Mouse) 12317, (Rat) 64202

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Function(s)

complement component C1q binding glycoprotein binding DNA binding mRNA binding integrin binding iron ion binding calcium ion binding protein binding zinc ion binding carbohydrate binding ubiquitin protein ligase binding peptide binding hormone binding protein binding involved in protein folding poly(A) RNA binding androgen receptor binding unfolded protein binding chaperone binding metal ion binding chaperone

Process(es)

negative regulation of transcription from RNA polymerase II promoter antigen processing and presentation of peptide antigen via MHC class I peptide antigen assembly with MHC class I protein complex regulation of transcription, DNA-templated protein folding protein export from nucleus cellular calcium ion homeostasis receptor-mediated endocytosis cell cycle arrest spermatogenesis positive regulation of cell proliferation positive regulation of gene expression negative regulation of translation protein maturation by protein folding cortical actin cytoskeleton organization response to estradiol negative regulation of intracellular steroid hormone receptor signaling pathway response to testosterone protein localization to nucleus protein folding in endoplasmic reticulum ATF6-mediated unfolded protein response regulation of meiotic nuclear division response to drug glucocorticoid receptor signaling pathway regulation of apoptotic process negative regulation of neuron differentiation positive regulation of DNA replication positive regulation of cell cycle negative regulation of transcription, DNA-templated negative regulation of retinoic acid receptor signaling pathway positive regulation of phagocytosis protein stabilization sequestering of calcium ion cardiac muscle cell differentiation chaperone-mediated protein folding cellular response to lithium ion cellular senescence positive regulation of substrate adhesion-dependent cell spreading positive regulation of dendritic cell chemotaxis response to organic substance cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calreticulin Polyclonal Antibody

Calreticulin Antibody (PA1-903) in WB

Product Details

PA1-903

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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