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Invitrogen
Calreticulin Recombinant Mouse Monoclonal Antibody (2E2)
FIGURE: 1 / 4
Calreticulin Antibody (MA5-50256) in ICC/IF
Immunocytochemistry analysis of HepG2 cell with Calreticulin monoclonal antibody (Product # MA5-50256) at 1:250, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Product Details
MA5-50256
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2E2
Immunogen
Recombinant Human CALR protein
Conjugate
Form
Liquid
Concentration
0.833 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_3093684
Target Information
Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Autoantigen RO; Calregulin; Calreticulin; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
Gene Aliases: CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA
UniProt ID: (Human) P27797, (Mouse) P14211
Entrez Gene ID: (Human) 811, (Mouse) 12317
complement component C1q binding
glycoprotein binding
DNA binding
mRNA binding
integrin binding
iron ion binding
calcium ion binding
protein binding
zinc ion binding
carbohydrate binding
ubiquitin protein ligase binding
peptide binding
hormone binding
protein binding involved in protein folding
poly(A) RNA binding
androgen receptor binding
unfolded protein binding
chaperone binding
metal ion binding
chaperone
negative regulation of transcription from RNA polymerase II promoter
antigen processing and presentation of peptide antigen via MHC class I
peptide antigen assembly with MHC class I protein complex
regulation of transcription, DNA-templated
protein folding
protein export from nucleus
cellular calcium ion homeostasis
receptor-mediated endocytosis
cell cycle arrest
spermatogenesis
positive regulation of cell proliferation
positive regulation of gene expression
negative regulation of translation
protein maturation by protein folding
cortical actin cytoskeleton organization
response to estradiol
negative regulation of intracellular steroid hormone receptor signaling pathway
response to testosterone
protein localization to nucleus
protein folding in endoplasmic reticulum
ATF6-mediated unfolded protein response
regulation of meiotic nuclear division
response to drug
glucocorticoid receptor signaling pathway
regulation of apoptotic process
negative regulation of neuron differentiation
positive regulation of DNA replication
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
positive regulation of phagocytosis
protein stabilization
sequestering of calcium ion
cardiac muscle cell differentiation
chaperone-mediated protein folding
cellular response to lithium ion
cellular senescence
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of dendritic cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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