Invitrogen

Caspase 1 p20 (Cleaved Asp296) Polyclonal Antibody

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Datasheet

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Antibody Testing Data (3)
Published Figures (7)

Caspase 1 p20 (Cleaved Asp296) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

Caspase 1 p20 (Cleaved Asp296) Antibody (PA5-99390) in IHC (P)

Immunohistochemistry analysis of Caspase 1 p20 (Cleaved Asp296) in rat heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.

Caspase 1 p20 (Cleaved Asp296) Antibody in Western Blot (WB)

Caspase 1 p20 (Cleaved Asp296) Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Caspase 1 p20 (Cleaved Asp296) Antibody in Immunohistochemistry (IHC)

Caspase 1 p20 (Cleaved Asp296) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

PA5-99390

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues I280-V299.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2818323

Product Specific Information

Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Asp296.

Target Information

Caspase-1 (CASP1) is a thiol protease that cleaves IL-1 beta between Asp and Ala. This cleavage causes the release of the mature cytokine which is involved in a variety of inflammatory processes. CASP1 is important for defense against pathogen. It cleaves and activates sterol regulatory element binding proteins (SREBPs). CASP1 also promotes apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, CASP1 controls antiviral immunity through the cleavage of CGAS and renders it inactive.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Breviscapine alleviates podocyte injury by inhibiting NF-κB/NLRP3-mediated pyroptosis in diabetic nephropathy.

PeerJ

Sun L,Ding M,Chen F,Zhu D,Xie X

PA5-99390 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that Bre alleviates HG-induced podocyte injury and improves renal function in diabetic mice, at least in part by inhibiting NF-κB/NLRP3-mediated pyroptosis.

Mon Feb 27 00:00:00 EST 2023

Salidroside Ameliorates Depression by Suppressing NLRP3-Mediated Pyroptosis via P2X7/NF-κB/NLRP3 Signaling Pathway.

Frontiers in pharmacology

Chai Y,Cai Y,Fu Y,Wang Y,Zhang Y,Zhang X,Zhu L,Miao M,Yan T

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Immunocytochemistry

Sat Jul 16 00:00:00 EDT 2022

Ticagrelor reduces doxorubicin-induced pyroptosis of rat cardiomyocytes by targeting GSK-3β/caspase-1.

Frontiers in cardiovascular medicine

Wang SH,Sun MJ,Ding SY,Liu CL,Wang JM,Han SN,Lin X,Li Q

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Immunohistochemistry

Wed Feb 01 00:00:00 EST 2023

Ticagrelor reduces doxorubicin-induced pyroptosis of rat cardiomyocytes by targeting GSK-3β/caspase-1.

Frontiers in cardiovascular medicine

Wang SH,Sun MJ,Ding SY,Liu CL,Wang JM,Han SN,Lin X,Li Q

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Immunohistochemistry

Wed Feb 01 00:00:00 EST 2023

Ropivacaine has the potential to relieve PM2.5-induced acute lung injury.

Experimental and therapeutic medicine

Zuo R,Li XY,He YG

PA5-99390 was used in Western Blotting to investigate the effect of ropivacaine on PM2.5-induced acute lung injury (ALI) and reveal the underlying mechanism.

Thu Sep 01 00:00:00 EDT 2022

GSDMD-dependent pyroptotic induction by a multivalent CXCR4-targeted nanotoxin blocks colorectal cancer metastases.

Drug delivery

Sala R,Rioja-Blanco E,Serna N,Sánchez-García L,Álamo P,Alba-Castellón L,Casanova I,López-Pousa A,Unzueta U,Céspedes MV,Vázquez E,Villaverde A,Mangues R

PA5-99390 was used in Immunohistochemistry to show that a CXCR4-dependent cytotoxic effect for T22-PE24-H6, which was not mediated by apoptosis, but instead capable of inducing a time-dependent and sequential activation of pyroptotic markers in CRC cells in vitro.

Thu Dec 01 00:00:00 EST 2022

Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation.

Innate immunity

Liu C,Cai B,Li D,Yao Y

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Western Blot

Sun Aug 01 00:00:00 EDT 2021

Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation.

Innate immunity

Liu C,Cai B,Li D,Yao Y

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Western Blot

Sun Aug 01 00:00:00 EDT 2021

Wolf-Hirschhorn syndrome candidate 1 facilitates alveolar macrophage pyroptosis in sepsis-induced acute lung injury through NEK7-mediated NLRP3 inflammasome activation.

Innate immunity

Liu C,Cai B,Li D,Yao Y

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Western Blot

Sun Aug 01 00:00:00 EDT 2021

Salidroside Ameliorates Alzheimer's Disease by Targeting NLRP3 Inflammasome-Mediated Pyroptosis.

Frontiers in aging neuroscience

Cai Y,Chai Y,Fu Y,Wang Y,Zhang Y,Zhang X,Zhu L,Miao M,Yan T

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Immunocytochemistry

Tue Feb 08 00:00:00 EST 2022

Salidroside Ameliorates Alzheimer's Disease by Targeting NLRP3 Inflammasome-Mediated Pyroptosis.

Frontiers in aging neuroscience

Cai Y,Chai Y,Fu Y,Wang Y,Zhang Y,Zhang X,Zhu L,Miao M,Yan T

Published figure using Caspase 1 p20 (Cleaved Asp296) polyclonal antibody (Product # PA5-99390) in Immunocytochemistry

Tue Feb 08 00:00:00 EST 2022

Gasdermin D in peripheral nerves: the pyroptotic microenvironment inhibits nerve regeneration.

Cell death discovery

Tao Y,Wang F,Xu Z,Lu X,Yang Y,Wu J,Yao C,Yi F,Li J,Huang Z,Liu Y

PA5-99390 was used in Western Blot to study the involvement of foamy cells in peripheral nerve regeneration in a GSDMD-/- mouse model.

Mon Jun 14 00:00:00 EDT 2021

MicroRNA-140-5p represses chondrocyte pyroptosis and relieves cartilage injury in osteoarthritis by inhibiting cathepsin B/Nod-like receptor protein 3.

Bioengineered

Zhang L,Qiu J,Shi J,Liu S,Zou H

PA5-99390 was used in Immunohistochemistry to investigate the mechanism of miR-140-5p in osteoarthritis cartilage injury.

Wed Dec 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

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Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45

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UniProt ID: (Human) P29466, (Mouse) P29452

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Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166

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Function(s)

endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type endopeptidase activator activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic process peptidase activity cysteine-type peptidase activity hydrolase activity scaffold protein binding protease

Process(es)

response to hypoxia proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction regulation of autophagy protein processing response to lipopolysaccharide interleukin-1 beta production response to ATP regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion regulation of inflammatory response mitochondrial depolarization membrane hyperpolarization pyroptosis cellular response to mechanical stimulus cellular response to organic substance programmed necrotic cell death toxin transport microglial cell activation myoblast fusion memory response to bacterium response to organic cyclic compound response to drug positive regulation of apoptotic process positive regulation of circadian sleep/wake cycle, non-REM sleep positive regulation of cytokine secretion midgut development response to toxic substance lung development execution phase of apoptosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Caspase 1 p20 (Cleaved Asp296) Polyclonal Antibody

Caspase 1 p20 (Cleaved Asp296) Antibody (PA5-99390) in IHC (P)

Product Details

PA5-99390

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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