Proteintech

Connexin 43 Polyclonal Antibody

Connexin 43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Connexin 43 Antibody (26980-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 26980-1-AP ( Connexin 43 Antibody) at dilution of 1:200 (under 40x lens).

Connexin 43 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Connexin 43 Antibody in Western Blot (WB)

Connexin 43 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

26980-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 30 publications 30 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 19 publications 19 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Connexin 43 Fusion Protein Ag25533 (232-382 aa encoded by BC026329)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FFKGVKDRV KGKSDPYHAT SGALSPAKDC GSQKYAYFNG CSSPTAPLSP MSPPGYKLVT GDRNNSSCRN YNKQASEQNW ANYSAEQNRM GQAGSTISNS HAQPFDFPDD NQNSKKLAAG HELQPLAIVD QRPSSRASSR ASSRPRPDDL EI (232-382 aa encoded by BC026329)

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Different Contacted Cell Types Contribute to Acquiring Different Properties in Brain Microglial Cells upon Intercellular Interaction.

International journal of molecular sciences

Nakano-Doi A,Kubo S,Sonoda E,Taguchi A,Nakagomi T

Mon Jan 16 00:00:00 EST 2023

Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions.

Cellular & molecular biology letters

Adzraku SY,Wang G,Cao C,Bao Y,Wang Y,Smith AO,Du Y,Wang H,Li Y,Xu K,Qiao J,Ju W,Zeng L

Mon Jan 16 00:00:00 EST 2023

Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions.

Cellular & molecular biology letters

Adzraku SY,Wang G,Cao C,Bao Y,Wang Y,Smith AO,Du Y,Wang H,Li Y,Xu K,Qiao J,Ju W,Zeng L

Mon Jan 16 00:00:00 EST 2023

Myelin Debris Impairs Tight Junctions and Promotes the Migration of Microvascular Endothelial Cells in the Injured Spinal Cord.

Cellular and molecular neurobiology

Yao F,Luo Y,Chen Y,Li Y,Hu X,You X,Li Z,Yu S,Tian D,Zheng M,Cheng L,Jing J

Wed Mar 01 00:00:00 EST 2023

Shensong Yangxin Capsule Reduces the Susceptibility of Arrhythmia in db/db Mice via Inhibiting the Inflammatory Response Induced by Endothelium Dysfunction.

Drug design, development and therapy

Zhang J,Li H,Wang D,Gu J,Hou Y,Wu Y

Tue Feb 14 00:00:00 EST 2023

Shensong Yangxin Capsule Reduces the Susceptibility of Arrhythmia in db/db Mice via Inhibiting the Inflammatory Response Induced by Endothelium Dysfunction.

Drug design, development and therapy

Zhang J,Li H,Wang D,Gu J,Hou Y,Wu Y

Tue Feb 14 00:00:00 EST 2023

Human Cytomegalovirus IE1 Impairs Neuronal Migration by Downregulating Connexin 43.

Journal of virology