Invitrogen

F4/80 Monoclonal Antibody (SP115)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

11 Published Figures

18 References

View all (67) F4/80 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

F4/80 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

F4/80 Antibody (MA5-16363) in ICC/IF

Immunofluorescence analysis of F4/80 was performed using 70% confluent log phase RAW 264.7 and NMuMG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with F4/80 Monoclonal Antibody (CI:A3-1) (Product # MA5-16363) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More

F4/80 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

F4/80 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

F4/80 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-F4/80 Monoclonal Antibody (SP115) (Product # MA5-16363) shows increased expression of F4/80 in RAW 264.7 cell line when compared to NMuMG. {RE}

Product Details

MA5-16363

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP115

Immunogen

Synthetic peptide derived from N-terminus of mouse F4/80 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537882

Product Specific Information

This antibody is predicted to react with rat based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. A suggested positive control is human lung or mouse liver tissue.

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.

Nature communications

Chou CW,Hung CN,Chiu CH,Tan X,Chen M,Chen CC,Saeed M,Hsu CW,Liss MA,Wang CM,Lai Z,Alvarez N,Osmulski PA,Gaczynska ME,Lin LL,Ortega V,Kirma NB,Xu K,Liu Z,Kumar AP,Taverna JA,Velagaleti GVN,Chen CL,Zhang Z,Huang TH

MA5-16363 was used in Immunocytochemistry to report that the reverse signaling using CD47 as a receptor additionally enhances a pro-survival function of prostate cancer cells under phagocytic attack.

Tue Oct 17 00:00:00 EDT 2023

Mitochondrial dysfunction is associated with lipid metabolism disorder and upregulation of angiotensin-converting enzyme 2.

PloS one

Zhao Q,Zhou X,Kuiper R,Curbo S,Karlsson A

MA5-16363 was used in Immunohistochemistry to suggest that lipoprotein lipase (LPL) from liver supports the metabolism when heart and skeletal muscle were impaired due to mitochondrial dysfunction.

Fri Jul 01 00:00:00 EDT 2022

Inflammation Mediates the Development of Aggressive Breast Cancer Following Radiotherapy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Ma L,Gonzalez-Junca A,Zheng Y,Ouyang H,Illa-Bochaca I,Horst KC,Krings G,Wang Y,Fernandez-Garcia I,Chou W,Barcellos-Hoff MH

MA5-16363 was used in Immunohistochemistry to investigate mechanisms by which radiation promotes aggressive cancer.

Mon Mar 15 00:00:00 EDT 2021

Sestrin Proteins Protect Against Lipotoxicity-Induced Oxidative Stress in the Liver via Suppression of C-Jun N-Terminal Kinases.

Cellular and molecular gastroenterology and hepatology

Fang Z,Kim HG,Huang M,Chowdhury K,Li MO,Liangpunsakul S,Dong XC

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry

Tue Mar 22 00:00:00 EDT 2022

A therapeutic vascular conduit to support in vivo cell-secreted therapy.

NPJ Regenerative medicine

Han EX,Qian H,Jiang B,Figetakis M,Kosyakova N,Tellides G,Niklason LE,Chang WG

MA5-16363 was used in Immunohistochemistry (Paraffin) to investigate the suitability of an approach for cell transplantation termed the Therapeutic Vascular Conduit (TVC), which uses an acellular vessel as a scaffold for a hydrogel sheath containing cells designed to secrete a therapeutic protein.

Thu Jul 29 00:00:00 EDT 2021

A therapeutic vascular conduit to support in vivo cell-secreted therapy.

NPJ Regenerative medicine

Han EX,Qian H,Jiang B,Figetakis M,Kosyakova N,Tellides G,Niklason LE,Chang WG

Thu Jul 29 00:00:00 EDT 2021

Serum Amyloid A Promotes Inflammation-Associated Damage and Tumorigenesis in a Mouse Model of Colitis-Associated Cancer.

Cellular and molecular gastroenterology and hepatology

Davis TA,Conradie D,Shridas P,de Beer FC,Engelbrecht AM,de Villiers WJS

MA5-16363 was used in Immunohistochemistry to investigate the role of acute-phase protein serum amyloid A in colitis-associated cancer.

Fri Mar 25 00:00:00 EDT 2022

Serum Amyloid A Promotes Inflammation-Associated Damage and Tumorigenesis in a Mouse Model of Colitis-Associated Cancer.

Cellular and molecular gastroenterology and hepatology

Davis TA,Conradie D,Shridas P,de Beer FC,Engelbrecht AM,de Villiers WJS

MA5-16363 was used in Immunohistochemistry to investigate the role of acute-phase protein serum amyloid A in colitis-associated cancer.

Fri Mar 25 00:00:00 EDT 2022

Colorectal Cancer Progression Is Potently Reduced by a Glucose-Free, High-Protein Diet: Comparison to Anti-EGFR Therapy.

Cancers

Skibbe K,Brethack AK,Sünderhauf A,Ragab M,Raschdorf A,Hicken M,Schlichting H,Preira J,Brandt J,Castven D,Föh B,Pagel R,Marquardt JU,Sina C,Derer S

MA5-16363 was used in Immunohistochemistry to found GFHPD to systemically dampen glycolysis activity, thereby reducing CRC progression with a similar efficacy to EGFR-directed antibody therapy.7'.

Fri Nov 19 00:00:00 EST 2021

Endocrine-Exocrine Signaling Drives Obesity-Associated Pancreatic Ductal Adenocarcinoma.

Cell

Chung KM,Singh J,Lawres L,Dorans KJ,Garcia C,Burkhardt DB,Robbins R,Bhutkar A,Cardone R,Zhao X,Babic A,Vayrynen SA,Dias Costa A,Nowak JA,Chang DT,Dunne RF,Hezel AF,Koong AC,Wilhelm JJ,Bellin MD,Nylander V,Gloyn AL,McCarthy MI,Kibbey RG,Krishnaswamy S,Wolpin BM,Jacks T,Fuchs CS,Muzumdar MD

MA5-16363 was used in Immunohistochemistry to argue that PDAC progression is driven by local obesity-associated changes in the tumor microenvironment and implicate endocrine-exocrine signaling beyond insulin in PDAC development.

Thu May 14 00:00:00 EDT 2020

SIRT6 Protects Against Liver Fibrosis by Deacetylation and Suppression of SMAD3 in Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Zhong X,Huang M,Kim HG,Zhang Y,Chowdhury K,Cai W,Saxena R,Schwabe RF,Liangpunsakul S,Dong XC

MA5-16363 was used in Immunohistochemistry, Immunocytochemistry to suggest that SIRT6 plays a critical role in the protection against NASH development and it may serve as a potential therapeutic target for NASH.

Fri Jul 09 00:00:00 EDT 2021

SIRT6 Protects Against Liver Fibrosis by Deacetylation and Suppression of SMAD3 in Hepatic Stellate Cells.

Cellular and molecular gastroenterology and hepatology

Zhong X,Huang M,Kim HG,Zhang Y,Chowdhury K,Cai W,Saxena R,Schwabe RF,Liangpunsakul S,Dong XC

Fri Jul 09 00:00:00 EDT 2021

Improved Multiplex Immunohistochemistry for Immune Microenvironment Evaluation of Mouse Formalin-Fixed, Paraffin-Embedded Tissues.

Journal of immunology (Baltimore, Md. : 1950)

Sorrelle N,Ganguly D,Dominguez ATA,Zhang Y,Huang H,Dahal LN,Burton N,Ziemys A,Brekken RA

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry

Tue Jan 01 00:00:00 EST 2019

Improved Multiplex Immunohistochemistry for Immune Microenvironment Evaluation of Mouse Formalin-Fixed, Paraffin-Embedded Tissues.

Journal of immunology (Baltimore, Md. : 1950)

Sorrelle N,Ganguly D,Dominguez ATA,Zhang Y,Huang H,Dahal LN,Burton N,Ziemys A,Brekken RA

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry

Tue Jan 01 00:00:00 EST 2019

Tofacitinib enhances delivery of antibody-based therapeutics to tumor cells through modulation of inflammatory cells.

JCI insight

Simon N,Antignani A,Hewitt SM,Gadina M,Alewine C,FitzGerald D

MA5-16363 was used in Immunohistochemistry to define the routes to which antibody-based therapeutics reach malignant cells.

Thu Mar 07 00:00:00 EST 2019

Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages.

Breast cancer research : BCR

Donzelli S,Milano E,Pruszko M,Sacconi A,Masciarelli S,Iosue I,Melucci E,Gallo E,Terrenato I,Mottolese M,Zylicz M,Zylicz A,Fazi F,Blandino G,Fontemaggi G

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry (Paraffin)

Tue Jun 19 00:00:00 EDT 2018

Phenotypical heterogeneity in responder and nonresponder male ApoE*3Leiden.CETP mice.

American journal of physiology. Gastrointestinal and liver physiology

Tarasco E,Pellegrini G,Whiting L,Lutz TA

MA5-16363 was used in Immunohistochemistry to assess the metabolism of a high fat, high cholesterol diet in ApoE*3Leiden.CETP mice, revealing highly variable responses resulting from two genetic phenotypes: responder (R) and nonresponder (NR) mice.

Mon Oct 01 00:00:00 EDT 2018

Effects of dextrose prolotherapy on contusion-induced muscle injuries in mice.

International journal of medical sciences

Tsai SW,Hsu YJ,Lee MC,Huang HE,Huang CC,Tung YT

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry (Paraffin)

Mon Jan 07 00:00:00 EST 2019

Revisiting the putative role of heme as a trigger of inflammation.

Pharmacology research & perspectives

Vallelian F,Schaer CA,Deuel JW,Ingoglia G,Humar R,Buehler PW,Schaer DJ

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry

Sun Apr 01 00:00:00 EDT 2018

Revisiting the putative role of heme as a trigger of inflammation.

Pharmacology research & perspectives

Vallelian F,Schaer CA,Deuel JW,Ingoglia G,Humar R,Buehler PW,Schaer DJ

Published figure using F4/80 monoclonal antibody (Product # MA5-16363) in Immunohistochemistry

Sun Apr 01 00:00:00 EDT 2018

GPR4 deficiency alleviates intestinal inflammation in a mouse model of acute experimental colitis.

Biochimica et biophysica acta. Molecular basis of disease

Sanderlin EJ,Leffler NR,Lertpiriyapong K,Cai Q,Hong H,Bakthavatchalu V,Fox JG,Oswald JZ,Justus CR,Krewson EA,O'Rourke D,Yang LV

MA5-16363 was used in Immunohistochemistry to suggest GPR4 provides a pro-inflammatory role in the inflamed gut as the absence of GPR4 ameliorates intestinal inflammation in the acute experimental colitis mouse model.

Wed Feb 01 00:00:00 EST 2017

Glutathione S-transferase alpha 4 induction by activator protein 1 in colorectal cancer.

Oncogene

Yang Y,Huycke MM,Herman TS,Wang X

MA5-16363 was used in immunohistochemistry - paraffin section to characterize the induction by activator protein 1 in colorectal cancer by glutathione S-transferase alpha 4

Thu Nov 03 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71

View more View less

Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3

View more View less

UniProt ID: (Mouse) Q61549

View more View less

Entrez Gene ID: (Mouse) 13733

View more View less

Function(s)

signal transducer activity transmembrane signaling receptor activity G-protein coupled receptor activity calcium ion binding G-protein coupled receptor

Process(es)

signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

F4/80 Monoclonal Antibody (SP115)

F4/80 Antibody (MA5-16363) in ICC/IF

Product Details

MA5-16363

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!