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Invitrogen
GSDMD Polyclonal Antibody
FIGURE: 1 / 6
GSDMD Antibody (PA5-30823) in WB
Western Blot analysis of GSDMD was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GSDMD Polyclonal Antibody (Product # PA5-30823) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Product Details
PA5-30823
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 200 of Human GSDMD
Conjugate
Form
Liquid
Concentration
1.31 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2548297
Product Specific Information
Recommended positive controls: A431.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D
Gene Aliases: DF5L; DFNA5L; FKSG10; GSDMD; GSDMDC1
UniProt ID: (Human) P57764
Entrez Gene ID: (Human) 79792
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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