Invitrogen

HIF1A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

26 Published Figures

44 References

View all (97) HIF1A antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

HIF1A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 34

HIF1A Antibody (PA1-16601) in ICC/IF

Immunofluorescent analysis of HIF-1 alpha (green) in HeLa cells either left untreated (left panel) or treated with 0.1 mM Cobalt Chloride (right panel) for 4 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a HIF-1 alpha polyclonal antibody (Product # PA1-16601) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 48... View More

HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

HIF1A Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

HIF1A Antibody in Immunohistochemistry (IHC)

HIF1A Antibody in Western Blot, Immunoprecipitation, ChIP Assay (WB, IP, ChIP)

HIF1A Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

HIF1A Antibody — Figure 8 HIF-1alpha protein levels were stabilized by AA treatment. HIF-1alpha protein levels were evaluated by western blot at different time-points upon AA treatment. (A) mESC incubated for 14h with CoCl 2 (300 uM) a known disruptor of prolyl hydroxylase activity, show increased Hif-1alpha protein levels. mESC without CoCl 2 treatment were used as control and different protein volumes were loaded in the gel. (B) Representative blot for E14 cells showing increased Hif-1alpha protein levels downstream to AA treatment. (C) Densiometric evaluation of HIF-1alpha and actin (loading control) bands show that a statistical significant difference exists between control and treated conditions at 2 and 4 hours for both E14 and R1 mESC. Data shown as protein level fold increase for each one of the AA conditions relative to the respective control. Data were normal and evaluated by one sample T Student tests against a theoretical value of 1. Error bars = SEM and statistical significance is considered when * p<0.05. {TM}

Product Details

PA1-16601

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 23 publications 23 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

5-10 µg/mL

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

1:10-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:10-1:500

View 3 publications 3 publications

ChIP assay (ChIP)

1:10-1:500

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Fish, Goat, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Dog, Human, Mouse, Rabbit, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2117128

Product Specific Information

This antibody detects upregulation of HIF-1 alpha in hypoxic samples.

Recommended positive controls: Human pancreas whole tissue lysate, COS-7 nuclear hypoxic induced cell lysate, human bone marrow whole tissue lysate (adult whole diabetes), HeLa hypoxic (CoCl2) cell lysate, HepG2 hypoxic (CoCl2) cell lysate, HeLa hypoxic cell Lysate, HepG2 hypoxic cell lysate, HIF-1 alpha knockout HeLa cell lysate

Target Information

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oxygen microcapsules improve immune checkpoint blockade by ameliorating hypoxia condition in pancreatic ductal adenocarcinoma.

Bioactive materials

Wu J,Wang X,Chen L,Wang J,Zhang J,Tang J,Ji Y,Song J,Wang L,Zhao Y,Zhang H,Li T,Sheng J,Chen D,Zhang Q,Liang T

PA1-16601 was used in Western Blotting to synthesize polydopamine-nanoparticle-stabilized oxygen microcapsules with excellent stabilization, bioavailability, and biocompatibility for direct oxygen delivery into tumor sites by interfacial polymerization.

Wed Feb 01 00:00:00 EST 2023

Metformin accelerates bone fracture healing by promoting type H vessel formation through inhibition of YAP1/TAZ expression.

Bone research

Ruan Z,Yin H,Wan TF,Lin ZR,Zhao SS,Long HT,Long C,Li ZH,Liu YQ,Luo H,Cheng L,Chen C,Zeng M,Lin ZY,Zhao RB,Chen CY,Wang ZX,Liu ZZ,Cao J,Wang YY,Jin L,Liu YW,Zhu GQ,Zou JT,Gong JS,Luo Y,Hu Y,Zhu Y,Xie H

PA1-16601 was used in Immunohistochemistry-immunofluorescence to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.

Wed Aug 16 00:00:00 EDT 2023

Hypoxia deactivates epigenetic feedbacks via enzyme-derived clicking proteolysis-targeting chimeras.

Science advances

Do TC,Lau JW,Sun C,Liu S,Kha KT,Lim ST,Oon YY,Kwan YP,Ma JJ,Mu Y,Liu X,Carney TJ,Wang X,Xing B

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Immunocytochemistry

Wed Dec 14 00:00:00 EST 2022

Hypoxia deactivates epigenetic feedbacks via enzyme-derived clicking proteolysis-targeting chimeras.

Science advances

Do TC,Lau JW,Sun C,Liu S,Kha KT,Lim ST,Oon YY,Kwan YP,Ma JJ,Mu Y,Liu X,Carney TJ,Wang X,Xing B

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Immunocytochemistry

Wed Dec 14 00:00:00 EST 2022

Immunorthodontics: PD-L1, a Novel Immunomodulator in Cementoblasts, Is Regulated by HIF-1α under Hypoxia.

Cells

Yong J,Gröger S,von Bremen J,Meyle J,Ruf S

PA1-16601 was used in Western Blotting to conclude that combined HIF-1α/PD-L1 inhibition could be of possible therapeutic relevance for OIIRR prevention.

Sat Jul 30 00:00:00 EDT 2022

Immunorthodontics: PD-L1, a Novel Immunomodulator in Cementoblasts, Is Regulated by HIF-1α under Hypoxia.

Cells

Yong J,Gröger S,von Bremen J,Meyle J,Ruf S

PA1-16601 was used in Western Blotting to conclude that combined HIF-1α/PD-L1 inhibition could be of possible therapeutic relevance for OIIRR prevention.

Sat Jul 30 00:00:00 EDT 2022

Immunorthodontics: PD-L1, a Novel Immunomodulator in Cementoblasts, Is Regulated by HIF-1α under Hypoxia.

Cells

Yong J,Gröger S,von Bremen J,Meyle J,Ruf S

PA1-16601 was used in Western Blotting to conclude that combined HIF-1α/PD-L1 inhibition could be of possible therapeutic relevance for OIIRR prevention.

Sat Jul 30 00:00:00 EDT 2022

Cobalt (II) Chloride Regulates the Invasion and Survival of Brucella abortus 544 in RAW 264.7 Cells and B6 Mice.

Pathogens (Basel, Switzerland)

Huy TXN,Nguyen TT,Reyes AWB,Kim H,Min W,Lee HJ,Lee JH,Kim S

PA1-16601 was used in Western Blotting to demonstrate that CoCl2 administration induced the production of serum cytokines IFN-γ and IL-6, which is accompanied by dampened Brucella proliferation in the spleen and liver of treated mice, and reduced the splenomegaly and hepatomegaly.

Wed May 18 00:00:00 EDT 2022

Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer.

Journal of experimental & clinical cancer research : CR

Salaroglio IC,Belisario DC,Akman M,La Vecchia S,Godel M,Anobile DP,Ortone G,Digiovanni S,Fontana S,Costamagna C,Rubinstein M,Kopecka J,Riganti C

PA1-16601 was used in Immunoprecipitation to suggest C/EBP-β LAP as a new predictive and prognostic factor in NSCLC patients.

Thu Aug 11 00:00:00 EDT 2022

Modulatory Effects of Estradiol and Its Mixtures with Ligands of GPER and PPAR on MAPK and PI3K/Akt Signaling Pathways and Tumorigenic Factors in Mouse Testis Explants and Mouse Tumor Leydig Cells.

Biomedicines

Gorowska-Wojtowicz E,Duliban M,Kotula-Balak M,Bilinska B

PA1-16601 was used in Western Blotting to suggest that a balanced estrogen level and its action together with proper GPER and PPAR signaling play a key role in the maintenance of testis homeostasis.

Sun Jun 12 00:00:00 EDT 2022

Organotypic whole hemisphere brain slice models to study the effects of donor age and oxygen-glucose-deprivation on the extracellular properties of cortical and striatal tissue.

Journal of biological engineering

McKenna M,Filteau JR,Butler B,Sluis K,Chungyoun M,Schimek N,Nance E

PA1-16601 was used in Immunohistochemistry-immunofluorescence to demonstrate whole hemisphere brain slices from P10 and P17 rats can be cultured up to two weeks in vitro.

Mon Jun 13 00:00:00 EDT 2022

Oxidation of Hemoglobin Drives a Proatherogenic Polarization of Macrophages in Human Atherosclerosis.

Antioxidants & redox signaling

Potor L,Hendrik Z,Patsalos A,Katona É,Méhes G,Póliska S,Csősz É,Kalló G,Komáromi I,Combi Z,Posta N,Sikura KÉ,Pethő D,Oros M,Vereb G,Tóth C,Gergely P,Nagy L,Balla G,Balla J

PA1-16601 was used in Western Blot to conclude that ferrylHb in atherosclerotic lesion provokes the progression of the disease via polarizing macrophages.

Wed Oct 20 00:00:00 EDT 2021

Oxidation of Hemoglobin Drives a Proatherogenic Polarization of Macrophages in Human Atherosclerosis.

Antioxidants & redox signaling

Potor L,Hendrik Z,Patsalos A,Katona É,Méhes G,Póliska S,Csősz É,Kalló G,Komáromi I,Combi Z,Posta N,Sikura KÉ,Pethő D,Oros M,Vereb G,Tóth C,Gergely P,Nagy L,Balla G,Balla J

PA1-16601 was used in Western Blot to conclude that ferrylHb in atherosclerotic lesion provokes the progression of the disease via polarizing macrophages.

Wed Oct 20 00:00:00 EDT 2021

Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model.

International journal of molecular sciences

Park M,Kim HM,Shin HA,Lee SH,Hwang DY,Lew H

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Western Blot

Sat Nov 20 00:00:00 EST 2021

LW1497, an Inhibitor of Malate Dehydrogenase, Suppresses TGF-β1-Induced Epithelial-Mesenchymal Transition in Lung Cancer Cells by Downregulating Slug.

Antioxidants (Basel, Switzerland)

Kim HJ,Park MK,Byun HJ,Kim M,Kim B,Yu L,Nguyen TM,Nguyen TH,Do PA,Kim EJ,Kim JH,Enkhtaivan E,Kim KS,Jang JY,Kang GJ,Lee H,Won M,Lee K,Cho J,Lee CH

PA1-16601 was used in Chromatin immunoprecipitation and Immunohistochemistry to show that LW1497 downregulated EMT markers and Slug. In conclusion, LW1497 suppresses cancer progression through the inhibition of EMT by downregulating Slug.

Sun Oct 24 00:00:00 EDT 2021

LW1497, an Inhibitor of Malate Dehydrogenase, Suppresses TGF-β1-Induced Epithelial-Mesenchymal Transition in Lung Cancer Cells by Downregulating Slug.

Antioxidants (Basel, Switzerland)

Kim HJ,Park MK,Byun HJ,Kim M,Kim B,Yu L,Nguyen TM,Nguyen TH,Do PA,Kim EJ,Kim JH,Enkhtaivan E,Kim KS,Jang JY,Kang GJ,Lee H,Won M,Lee K,Cho J,Lee CH

Sun Oct 24 00:00:00 EDT 2021

Silicon Oxynitrophosphide Nanoscale Coating Enhances Antioxidant Marker-Induced Angiogenesis During in vivo Cranial Bone-Defect Healing.

JBMR plus

do Monte FA,Ahuja N,Awad KR,Pan Z,Young S,Kim HK,Aswath P,Brotto M,Varanasi VG

PA1-16601 was used in Immunohistochemistry to test the angiogenic and antioxidant properties of amorphous silicon oxynitrophosphide (SiONPx) nanoscale-coating material on endothelial cells to regenerate vascular tissue.

Thu Apr 01 00:00:00 EDT 2021

Silicon Oxynitrophosphide Nanoscale Coating Enhances Antioxidant Marker-Induced Angiogenesis During in vivo Cranial Bone-Defect Healing.

JBMR plus

do Monte FA,Ahuja N,Awad KR,Pan Z,Young S,Kim HK,Aswath P,Brotto M,Varanasi VG

Thu Apr 01 00:00:00 EDT 2021

Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhao L,Law NC,Gomez NA,Son J,Gao Y,Liu X,de Avila JM,Zhu MJ,Du M

PA1-16601 was used in Western Blotting to demonstrate that MO induces hypoxia and impairs myogenic differentiation by up-regulating BMP signaling within the DM, which may account for the disruptions of skeletal muscle development and function in progeny.

Mon Nov 01 00:00:00 EDT 2021

Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhao L,Law NC,Gomez NA,Son J,Gao Y,Liu X,de Avila JM,Zhu MJ,Du M

Mon Nov 01 00:00:00 EDT 2021

Hypertrophic signaling compensates for contractile and metabolic consequences of DNA methyltransferase 3A loss in human cardiomyocytes.

Journal of molecular and cellular cardiology

Madsen A,Krause J,Höppner G,Hirt MN,Tan WLW,Lim I,Hansen A,Nikolaev VO,Foo RSY,Eschenhagen T,Stenzig J

PA1-16601 was used in Western Blotting to analyze the role of DNMT3A in the setting of cardiac hypertrophy.

Sat May 01 00:00:00 EDT 2021

An Important Role for DNMT3A-Mediated DNA Methylation in Cardiomyocyte Metabolism and Contractility.

Circulation

Madsen A,Höppner G,Krause J,Hirt MN,Laufer SD,Schweizer M,Tan WLW,Mosqueira D,Anene-Nzelu CG,Lim I,Foo RSY,Hansen A,Eschenhagen T,Stenzig J

PA1-16601 was used in Western Blotting to investigate the role of DNA methylation in the normal homeostasis of cardiomyocytes and during cardiac stress.

Tue Oct 20 00:00:00 EDT 2020

An Important Role for DNMT3A-Mediated DNA Methylation in Cardiomyocyte Metabolism and Contractility.

Circulation

Madsen A,Höppner G,Krause J,Hirt MN,Laufer SD,Schweizer M,Tan WLW,Mosqueira D,Anene-Nzelu CG,Lim I,Foo RSY,Hansen A,Eschenhagen T,Stenzig J

PA1-16601 was used in Western Blotting to investigate the role of DNA methylation in the normal homeostasis of cardiomyocytes and during cardiac stress.

Tue Oct 20 00:00:00 EDT 2020

Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit.

Neuron

Kullmann JA,Trivedi N,Howell D,Laumonnerie C,Nguyen V,Banerjee SS,Stabley DR,Shirinifard A,Rowitch DH,Solecki DJ

PA1-16601 was used in Western Blot, Immunocytochemistry to report that oxygen tension, which rises at birth, and the von Hippel-Lindau (VHL)-hypoxia-inducible factor 1α (Hif1α) pathway regulate polarization and maturation of post-mitotic cerebellar granule neurons (CGNs).

Wed May 20 00:00:00 EDT 2020

Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit.

Neuron

Kullmann JA,Trivedi N,Howell D,Laumonnerie C,Nguyen V,Banerjee SS,Stabley DR,Shirinifard A,Rowitch DH,Solecki DJ

Wed May 20 00:00:00 EDT 2020

YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells.

eLife

Sivaraj KK,Dharmalingam B,Mohanakrishnan V,Jeong HW,Kato K,Schröder S,Adams S,Koh GY,Adams RH

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in ChIP assay

Mon Jan 20 00:00:00 EST 2020

YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells.

eLife

Sivaraj KK,Dharmalingam B,Mohanakrishnan V,Jeong HW,Kato K,Schröder S,Adams S,Koh GY,Adams RH

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in ChIP assay

Mon Jan 20 00:00:00 EST 2020

YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells.

eLife

Sivaraj KK,Dharmalingam B,Mohanakrishnan V,Jeong HW,Kato K,Schröder S,Adams S,Koh GY,Adams RH

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in ChIP assay

Mon Jan 20 00:00:00 EST 2020

Maternal erythrocyte ENT1-mediated AMPK activation counteracts placental hypoxia and supports fetal growth.

JCI insight

Sayama S,Song A,Brown BC,Couturier J,Cai X,Xu P,Chen C,Zheng Y,Iriyama T,Sibai B,Longo M,Kellems RE,D'Alessandro A,Xia Y

PA1-16601 was used in Immunohistochemistry-immunofluorescence to demonstrate that the maternal eENT1–dependent uptake of extracellular adenosine signaling plays a role in delivering adequate O2 to the placentas.

Thu May 21 00:00:00 EDT 2020

Clinicopathological Analysis of HIF-1alpha and TERT on Survival Outcome in Glioblastoma Patients: A Prospective, Single Institution Study.

Journal of Cancer

Potharaju M,Mathavan A,Mangaleswaran B,Patil S,John R,Ghosh S,Kalavakonda C,Ghosh M,Verma RS

PA1-16601 was used in Immunohistochemistry to investigate the association of immunohistochemical expression of hypoxia inducible factor-1 alpha (HIF-1α), telomerase reverse transcriptase (TERT), isocitrate dehydrogenase 1 (IDH1) and tumor protein p53 with overall survival (OS) in glioblastoma patients.

Thu Oct 01 00:00:00 EDT 2020

Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.

International journal of molecular sciences

Hsu HL,Liao PL,Cheng YW,Huang SH,Wu CH,Li CH,Kang JJ

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Immunoprecipitation

Thu Jan 03 00:00:00 EST 2019

Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.

International journal of molecular sciences

Hsu HL,Liao PL,Cheng YW,Huang SH,Wu CH,Li CH,Kang JJ

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Immunoprecipitation

Thu Jan 03 00:00:00 EST 2019

Enantioselective Synthesis of 8-Hydroxyquinoline Derivative, Q134 as a Hypoxic Adaptation Inducing Agent.

Molecules (Basel, Switzerland)

Hackler L,Gyuris M,Huzián O,Alföldi R,Szebeni GJ,Madácsi R,Knapp L,Kanizsai I,Puskás LG

PA1-16601 was used in Western Blotting to show that the enantiomers of Q134 showed potent and similar activity in all tested assays.

Sat Nov 23 00:00:00 EST 2019

Macrophage-mediated delivery of light activated nitric oxide prodrugs with spatial, temporal and concentration control.

Chemical science

Evans MA,Huang PJ,Iwamoto Y,Ibsen KN,Chan EM,Hitomi Y,Ford PC,Mitragotri S

PA1-16601 was used in Flow cytometry/Cell sorting to report a "Trojan Horse" strategy to provide the necessary spatial, temporal, and dosage control of such drug-delivery therapies at targeted tissues.

Sat Apr 21 00:00:00 EDT 2018

Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves.

American journal of physiology. Heart and circulatory physiology

Amofa D,Hulin A,Nakada Y,Sadek HA,Yutzey KE

PA1-16601 was used in Immunohistochemistry-immunofluorescence to demonstrate that hypoxia maintains a primitive extracellular matrix during heart valve development and promotes extracellular matrix remodeling in adult mice, as occurs in myxomatous disease.

Fri Dec 01 00:00:00 EST 2017

Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia.

Investigative ophthalmology & visual science

Meng Q,Qin Y,Deshpande M,Kashiwabuchi F,Rodrigues M,Lu Q,Ren H,Elisseeff JH,Semenza GL,Montaner SV,Sodhi A

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Western Blot

Fri Sep 01 00:00:00 EDT 2017

Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.

Frontiers in pharmacology

Xiao N,Yang LL,Yang YL,Liu LW,Li J,Liu B,Liu K,Qi LW,Li P

PA116601 was used in western blot to determine the effect of ginsenoside Rg5 on adipose dysfunction and muscle insulin resistance

Wed Sep 30 00:00:00 EDT 2020

Inhibition of HIF Reduces Bladder Hypertrophy and Improves Bladder Function in Murine Model of Partial Bladder Outlet Obstruction.

The Journal of urology

Iguchi N,Malykhina AP,Wilcox DT

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2016

Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue.

Cellular signalling

Zhao W,Li A,Feng X,Hou T,Liu K,Liu B,Zhang N

PA1-16601 was used in western blot to characterize prevention of lipolysis and inflammation in hypoxic adipose tissue by studying metformin and resveratrol that ameliorate muscle insulin resistance

Thu Sep 01 00:00:00 EDT 2016

The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats.

Inflammation

Guven M,Sehitoglu MH,Yuksel Y,Tokmak M,Aras AB,Akman T,Golge UH,Karavelioglu E,Bal E,Cosar M

PA1-16601 was used in immunohistochemistry - paraffin section to demonstrate that coumaric acid is protective after a ischemia-reperfusion injury of the spinal cord.

Thu Oct 01 00:00:00 EDT 2015

Effect of CoCl₂ on fracture repair in a rat model of bone fracture.

Molecular medicine reports

Huang J,Liu L,Feng M,An S,Zhou M,Li Z,Qi J,Shen H

PA116601 was used in western blot to elucidate the contributions of low oxygen in fracture healing impairment

Thu Oct 01 00:00:00 EDT 2015

Snail Is a Direct Target of Hypoxia-inducible Factor 1α (HIF1α) in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells.

The Journal of biological chemistry

Xu X,Tan X,Tampe B,Sanchez E,Zeisberg M,Zeisberg EM

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Western Blot

Fri Jul 03 00:00:00 EDT 2015

K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy.

Scientific reports

Ferreira JV,Soares AR,Ramalho JS,Pereira P,Girao H

PA1-16601 was used in Western Blot to show that K63 ubiquitination is necessary for chaperone-mediated Autophagy degradation of HIF1A transcription factor in vitro and in vivo.

Mon May 11 00:00:00 EDT 2015

Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer.

The EMBO journal

Pate KT,Stringari C,Sprowl-Tanio S,Wang K,TeSlaa T,Hoverter NP,McQuade MM,Garner C,Digman MA,Teitell MA,Edwards RA,Gratton E,Waterman ML

PA1-16601 was used in immunohistochemistry to study the role of PDK1 in the mechanism by which Wnt signaling modulates glycolytic flux and angiogenesis in colon cancer

Tue Jul 01 00:00:00 EDT 2014

HIF-1α-induced HSP70 regulates anabolic responses in articular chondrocytes under hypoxic conditions.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Tsuchida S,Arai Y,Takahashi KA,Kishida T,Terauchi R,Honjo K,Nakagawa S,Inoue H,Ikoma K,Ueshima K,Matsuki T,Mazda O,Kubo T

Published figure using HIF1A polyclonal antibody (Product # PA1-16601) in Western Blot

Fri Aug 01 00:00:00 EDT 2014

Response of small heat shock proteins in diabetic rat retina.

Investigative ophthalmology & visual science

Reddy VS,Raghu G,Reddy SS,Pasupulati AK,Suryanarayana P,Reddy GB

PA1-16601 was used in western blot to study the expression of small heat shock proteins in the retina of diabetic rats

Tue Nov 19 00:00:00 EST 2013

Inhibition of mitochondrial complex III blocks neuronal differentiation and maintains embryonic stem cell pluripotency.

PloS one

Pereira SL,Grãos M,Rodrigues AS,Anjo SI,Carvalho RA,Oliveira PJ,Arenas E,Ramalho-Santos J

PA1-16601 was used in western blot to study the suppression of the neuronal differentiation of murine embryonic stem cells following mitochondrial complex III inhibition

Sat Sep 27 00:00:00 EDT 2014

Thermal dose fractionation affects tumour physiological response.

International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group

Thrall DE,Maccarini P,Stauffer P,Macfall J,Hauck M,Snyder S,Case B,Linder K,Lan L,McCall L,Dewhirst MW

PA1-16601 was used in Immunohistochemistry to assess the effect of administering the same total thermal dose, approximately 30 CEM43T(90), in one versus three to four fractions per week, over 5 weeks.

Tue Dec 04 00:00:00 EST 2012

Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells.

The Journal of endocrinology

Ishizuka T,Hinata T,Watanabe Y

PA1-16601 was used in western blot to investigate the interplay among high-glucose content, oxidative stress, and production of angiogenic growth factors in mesenchymal stem cells

Tue Feb 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8

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Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

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UniProt ID: (Human) Q16665, (Mouse) Q61221, (Rat) O35800

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Entrez Gene ID: (Pig) 396696, (Human) 3091, (Goat) 100861391, (Dog) 480348, (Rabbit) 100009579, (Mouse) 15251, (Rat) 29560, (Chinese hamster) 100751293

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Function(s)

transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding histone acetyltransferase binding nuclear hormone receptor binding histone deacetylase binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding signal transducer activity protein dimerization activity double-stranded DNA binding basic helix-loop-helix transcription factor

Process(es)

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HIF1A Polyclonal Antibody

HIF1A Antibody (PA1-16601) in ICC/IF

Product Details

PA1-16601

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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