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Histone H3K9me3 Polyclonal Antibody

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Antibody Testing Data (5)

Histone H3K9me3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

Histone H3K9me3 Antibody (39161) in ICC/IF

Histone H3 trimethyl Lys9 antibody tested by immunofluorescence. HeLa cells stained at 2 µg/mL with Histone H3 trimethyl Lys9 antibody. Top panel: Histone H3 trimethyl Lys9 antibody. Middle panel: DAPI. Bottom panel: merge.

Histone H3K9me3 Antibody in Western Blot (WB)

Histone H3K9me3 Antibody in ChIP-Sequencing (ChIP-Seq)

Histone H3K9me3 Antibody in ELISA (ELISA)

Histone H3K9me3 Antibody in Dot Blot (DB)

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Product Details

39161

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:500-1:1,000

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

ChIP assay (ChIP)

2-5 µL

View 8 publications 8 publications

ChIP-sequencing (ChIP-Seq)

5 µL

View 8 publications 8 publications

Dot blot (DB)

Assay-dependent

Miscellaneous PubMed (Misc)

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Yeast

Published species

Fungi, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2532132

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Oscillatory cAMP signaling rapidly alters H3K4 methylation.

Life science alliance

Huff TC,Camarena V,Sant DW,Wilkes Z,Van Booven D,Aron AT,Muir RK,Renslo AR,Chang CJ,Monje PV,Wang G

Research topic: Histone Methylation

Wed Jan 01 00:00:00 EST 2020

Comprehensive Analysis of Chromatin States in Atypical Teratoid/Rhabdoid Tumor Identifies Diverging Roles for SWI/SNF and Polycomb in Gene Regulation.

Cancer cell

Erkek S,Johann PD,Finetti MA,Drosos Y,Chou HC,Zapatka M,Sturm D,Jones DTW,Korshunov A,Rhyzova M,Wolf S,Mallm JP,Beck K,Witt O,Kulozik AE,Frühwald MC,Northcott PA,Korbel JO,Lichter P,Eils R,Gajjar A,Roberts CWM,Williamson D,Hasselblatt M,Chavez L,Pfister SM,Kool M

Research topic: atypical teratoid rhabdoid tumors (ATRT), Gene Regulation

Mon Jan 14 00:00:00 EST 2019

SMYD3 promotes the epithelial-mesenchymal transition in breast cancer.

Nucleic acids research

Fenizia C,Bottino C,Corbetta S,Fittipaldi R,Floris P,Gaudenzi G,Carra S,Cotelli F,Vitale G,Caretti G

Research topic: Breast Cancer

Wed Feb 20 00:00:00 EST 2019

IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP.

eLife

Roy A,Ghosh A,Kumar B,Chandran B

Research topic: Virology, KSHV

Mon Nov 04 00:00:00 EST 2019

Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses.

Cancer cell

Stewart E,McEvoy J,Wang H,Chen X,Honnell V,Ocarz M,Gordon B,Dapper J,Blankenship K,Yang Y,Li Y,Shaw TI,Cho JH,Wang X,Xu B,Gupta P,Fan Y,Liu Y,Rusch M,Griffiths L,Jeon J,Freeman BB,Clay MR,Pappo A,Easton J,Shurtleff S,Shelat A,Zhou X,Boggs K,Mulder H,Yergeau D,Bahrami A,Mardis ER,Wilson RK,Zhang J,Peng J,Downing JR,Dyer MA

Research topic: Skeletal Muscle - Rhabdomyosarcoma

Mon Sep 10 00:00:00 EDT 2018

SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice.

Developmental cell

Hirota T,Blakeley P,Sangrithi MN,Mahadevaiah SK,Encheva V,Snijders AP,ElInati E,Ojarikre OA,de Rooij DG,Niakan KK,Turner JMA

Research topic: DNA Damage & Repair

Mon Dec 03 00:00:00 EST 2018

Switching roles for DNA and histone methylation depend on evolutionary ages of human endogenous retroviruses.

Genome research

Ohtani H,Liu M,Zhou W,Liang G,Jones PA

Research topic: DNA Methyation

Wed Aug 01 00:00:00 EDT 2018

Loss of UHRF2 Is Associated With Non-small Cell Lung Carcinoma Progression.

Journal of Cancer

Jin C,Xiong D,Li HR,Jiang JH,Qi JC,Ding JY

Research topic: Neurology - Glioblastoma

Thu Sep 28 00:00:00 EDT 2023

A comprehensive map coupling histone modifications with gene regulation in adult dopaminergic and serotonergic neurons.

Nature communications

Södersten E,Toskas K,Rraklli V,Tiklova K,Björklund ÅK,Ringnér M,Perlmann T,Holmberg J

Research topic: Brain & Nerves

Mon Mar 26 00:00:00 EDT 2018

The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis.

Neuron

Aldiri I,Xu B,Wang L,Chen X,Hiler D,Griffiths L,Valentine M,Shirinifard A,Thiagarajan S,Sablauer A,Barabas ME,Zhang J,Johnson D,Frase S,Zhou X,Easton J,Zhang J,Mardis ER,Wilson RK,Downing JR,Dyer MA

Research topic: Eyes

Wed May 03 00:00:00 EDT 2017

The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression.

Scientific reports

Gonzalez-Vasconcellos I,Schneider R,Anastasov N,Alonso-Rodriguez S,Sanli-Bonazzi B,Fernández JL,Atkinson MJ

Research topic: Osteosarcoma, Telomeres

Tue Feb 07 00:00:00 EST 2017

Identification of the elementary structural units of the DNA damage response.

Nature communications

Natale F,Rapp A,Yu W,Maiser A,Harz H,Scholl A,Grulich S,Anton T,Hörl D,Chen W,Durante M,Taucher-Scholz G,Leonhardt H,Cardoso MC

Research topic: DNA Damage Response

Mon Jun 12 00:00:00 EDT 2017

Probing the impact of chromatin conformation on genome editing tools.

Nucleic acids research

Chen X,Rinsma M,Janssen JM,Liu J,Maggio I,Gonçalves MA

Wed Jul 27 00:00:00 EDT 2016

Loss of HP1 causes depletion of H3K27me3 from facultative heterochromatin and gain of H3K27me2 at constitutive heterochromatin.

Genome research

Jamieson K,Wiles ET,McNaught KJ,Sidoli S,Leggett N,Shao Y,Garcia BA,Selker EU

Research topic: Epigenetics in mold

Fri Jan 01 00:00:00 EST 2016

HCF1 and OCT2 Cooperate with EBNA1 To Enhance OriP-Dependent Transcription and Episome Maintenance of Latent Epstein-Barr Virus.

Journal of virology

Dheekollu J,Wiedmer A,Sentana-Lledo D,Cassel J,Messick T,Lieberman PM

Research topic: Virology - EBV

Wed Jun 01 00:00:00 EDT 2016

Alterations in nuclear structure promote lupus autoimmunity in a mouse model.

Disease models & mechanisms

Singh N,Johnstone DB,Martin KA,Tempera I,Kaplan MJ,Denny MF

Research topic: Autoimmunity - Lupus (SLE)

Mon Aug 01 00:00:00 EDT 2016

Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An in vitro study.

World journal of gastroenterology

Das D,Sarkar N,Sengupta I,Pal A,Saha D,Bandopadhyay M,Das C,Narayan J,Singh SP,Chakravarty R

Research topic: Hepatitis B

Wed Dec 21 00:00:00 EST 2016

Identification of key factors conquering developmental arrest of somatic cell cloned embryos by combining embryo biopsy and single-cell sequencing.

Cell discovery

Liu W,Liu X,Wang C,Gao Y,Gao R,Kou X,Zhao Y,Li J,Wu Y,Xiu W,Wang S,Yin J,Liu W,Cai T,Wang H,Zhang Y,Gao S

Research topic: Cell Cloning

Wed Jul 27 00:00:00 EDT 2016

An Alternative Approach to ChIP-Seq Normalization Enables Detection of Genome-Wide Changes in Histone H3 Lysine 27 Trimethylation upon EZH2 Inhibition.

PloS one

Egan B,Yuan CC,Craske ML,Labhart P,Guler GD,Arnott D,Maile TM,Busby J,Henry C,Kelly TK,Tindell CA,Jhunjhunwala S,Zhao F,Hatton C,Bryant BM,Classon M,Trojer P

Thu Jun 22 00:00:00 EDT 2017

Intracellular α-ketoglutarate maintains the pluripotency of embryonic stem cells.

Nature

Carey BW,Finley LW,Cross JR,Allis CD,Thompson CB

Research topic: Stem Cells

Thu Feb 19 00:00:00 EST 2015

Histone methylation patterns in astrocytes are influenced by age following ischemia.

Epigenetics

Chisholm NC,Henderson ML,Selvamani A,Park MJ,Dindot S,Miranda RC,Sohrabji F

Research topic: Brain & Nerves

Mon Oct 05 00:00:00 EDT 2015

Chromatin analyses of Zymoseptoria tritici: Methods for chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq).

Fungal genetics and biology : FG & B

Soyer JL,Möller M,Schotanus K,Connolly LR,Galazka JM,Freitag M,Stukenbrock EH

Research topic: Methods - ChIP in fungi

Mon Jun 01 00:00:00 EDT 2015

Chromatin analyses of Zymoseptoria tritici: Methods for chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq).

Fungal genetics and biology : FG & B

Soyer JL,Möller M,Schotanus K,Connolly LR,Galazka JM,Freitag M,Stukenbrock EH

Research topic: Methods - ChIP in fungi

Mon Jun 01 00:00:00 EDT 2015

hnRNP K coordinates transcriptional silencing by SETDB1 in embryonic stem cells.

PLoS genetics

Thompson PJ,Dulberg V,Moon KM,Foster LJ,Chen C,Karimi MM,Lorincz MC

Research topic: Virology, Stem Cells, Transcriptional Regulation

Thu Jan 01 00:00:00 EST 2015

MoSET1 (Histone H3K4 Methyltransferase in Magnaporthe oryzae) Regulates Global Gene Expression during Infection-Related Morphogenesis.

PLoS genetics

Pham KT,Inoue Y,Vu BV,Nguyen HH,Nakayashiki T,Ikeda K,Nakayashiki H

Research topic: Fungus Pathogenicity

Wed Jul 01 00:00:00 EDT 2015

An ultra-low-input native ChIP-seq protocol for genome-wide profiling of rare cell populations.

Nature communications

Brind'Amour J,Liu S,Hudson M,Chen C,Karimi MM,Lorincz MC

Research topic: Methods

Wed Jan 21 00:00:00 EST 2015

Histone modifications change with age, dietary restriction and rapamycin treatment in mouse brain.

Oncotarget

Gong H,Qian H,Ertl R,Astle CM,Wang GG,Harrison DE,Xu X

Research topic: Neurodegeneration, Alzheimers, Parkinson, Huntington

Tue Jun 30 00:00:00 EDT 2015

Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment.

Cell reports

Cooper S,Dienstbier M,Hassan R,Schermelleh L,Sharif J,Blackledge NP,De Marco V,Elderkin S,Koseki H,Klose R,Heger A,Brockdorff N

Research topic: Stem Cells

Thu Jun 12 00:00:00 EDT 2014

The Mbd1-Atf7ip-Setdb1 pathway contributes to the maintenance of X chromosome inactivation.

Epigenetics & chromatin

Minkovsky A,Sahakyan A,Rankin-Gee E,Bonora G,Patel S,Plath K

Research topic: Embryogenesis, X chromosome inactivation (XCI)

Wed Jul 16 00:00:00 EDT 2014

Epigenetic control of effector gene expression in the plant pathogenic fungus Leptosphaeria maculans.

PLoS genetics

Soyer JL,El Ghalid M,Glaser N,Ollivier B,Linglin J,Grandaubert J,Balesdent MH,Connolly LR,Freitag M,Rouxel T,Fudal I

Research topic: Chromatin Modification

Sat Mar 01 00:00:00 EST 2014

Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells.

Proceedings of the National Academy of Sciences of the United States of America

Murphy PJ,Cipriany BR,Wallin CB,Ju CY,Szeto K,Hagarman JA,Benitez JJ,Craighead HG,Soloway PD

Research topic: Methods Paper

Tue May 07 00:00:00 EDT 2013

Contribution of myocyte enhancer factor 2 family transcription factors to BZLF1 expression in Epstein-Barr virus reactivation from latency.

Journal of virology

Murata T,Narita Y,Sugimoto A,Kawashima D,Kanda T,Tsurumi T

Research topic: Virology (EBV)

Sun Sep 01 00:00:00 EDT 2013

Distinct roles of KAP1, HP1 and G9a/GLP in silencing of the two-cell-specific retrotransposon MERVL in mouse ES cells.

Epigenetics & chromatin

Maksakova IA,Thompson PJ,Goyal P,Jones SJ,Singh PB,Karimi MM,Lorincz MC

Research topic: Gene Regulation

Tue Jun 04 00:00:00 EDT 2013

BZLF1 governs CpG-methylated chromatin of Epstein-Barr Virus reversing epigenetic repression.

PLoS pathogens

Woellmer A,Arteaga-Salas JM,Hammerschmidt W

Research topic: Transcription Regulation

Sat Sep 01 00:00:00 EDT 2012

Epigenetic histone modification of Epstein-Barr virus BZLF1 promoter during latency and reactivation in Raji cells.

Journal of virology

Murata T,Kondo Y,Sugimoto A,Kawashima D,Saito S,Isomura H,Kanda T,Tsurumi T

Research topic: Virology (EBV)

Tue May 01 00:00:00 EDT 2012

Synergistic cytotoxicity of the DNA alkylating agent busulfan, nucleoside analogs and suberoylanilide hydroxamic acid in lymphoma cell lines.

Leukemia & lymphoma

Valdez BC,Murray D,Nieto Y,Li Y,Wang G,Champlin RE,Andersson BS

Research topic: Cancer, Lymphoma

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 K9; H3-clustered histone 13; H3-clustered histone 14; H3-clustered histone 15; H3/b; H3/d; H3/f; H3/j; H3K9; H3Lys9me3; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 1, H3j; histone 2, H3a; histone 2, H3c; Histone 3; Histone H3; Histone H3.1; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o; Histone H3K9me3; methyl Histone 3; methyl Histone H3; Tri-methyl H3; Tri-methyl Histone H3; Tri-Methyl-H3 K9; Tri-Methyl-H3 Lys9; Tri-Methyl-Histone H3 K9; Tri-Methyl-Histone H3 Lys9; Trimethyl H3; Trimethyl Histone H3

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Gene Aliases: BUR5; H3; H3.2; H3/A; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3C1; H3C10; H3C11; H3C12; H3C13; H3C14; H3C15; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3F1K; H3F2; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; HHT1; HHT2; HIST1H3A; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; N2749; SIN2; YBR010W; YBR0201; YNL031C

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UniProt ID: (Baker's yeast) P61830, (Human) P68431, (Human) Q71DI3

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Entrez Gene ID: (Baker's yeast) 852295, (Human) 8350, (Human) 8358, (Human) 8968, (Human) 8357, (Human) 8356, (Human) 333932, (Human) 126961

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Function(s)

DNA binding cupric reductase activity structural constituent of chromatin protein heterodimerization activity protein binding histone binding cadherin binding involved in cell-cell adhesion histone

Process(es)

chromatin organization cellular copper ion homeostasis aerobic respiration rRNA transcription sexual sporulation resulting in formation of a cellular spore global genome nucleotide-excision repair chromatin silencing at rDNA positive regulation of defense response to virus by host nucleosome assembly DNA replication-dependent nucleosome assembly blood coagulation gene silencing by RNA telomere organization cellular protein metabolic process negative regulation of gene expression, epigenetic positive regulation of gene expression, epigenetic protein heterotetramerization regulation of gene silencing cell-cell adhesion xenophagy

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Histone H3K9me3 Polyclonal Antibody

Histone H3K9me3 Antibody (39161) in ICC/IF

Product Details

39161

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

Dot blot (DB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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