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IDH1 Antibody (PA5-142665) in WB
Western Blot analysis of IDH1 in S. cerevisiae S288c lysate (35 µg protein in RIPA buffer) using IDH1 Polyclonal Antibody (Product # PA5-142665) at 0.5 µg/mL. Primary incubation was 1 hour. Detected by chemiluminescence. The blocking buffer contained non-animal derived protein. Data kindly provided by Dr. F Reggiori, University of Utrecht, Netherlands.
Product Details
PA5-142665
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-EPGSRHVGLDIKGQN.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2933311
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:16,000.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial; Isocitric dehydrogenase; NAD(+)-specific ICDH; NADP+-specific ICDH; OTTHUMP00000206464; OTTHUMP00000206465; OTTHUMP00000206466
Gene Aliases: IDH1; N2690; YNL037C
UniProt ID: (Baker's yeast) P28834
Entrez Gene ID: (Baker's yeast) 855691
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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