Invitrogen

IL-6 Monoclonal Antibody (7IL6), Biotin

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

21 References

View all (212) IL-6 antibodies

Datasheet

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Datasheet

Protocols

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IL-6 Antibody (M621B) in ELISA

Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 µL per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 µg/mL and 50 µL of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/mL (both in quadruplicate) across a 4 µg/mL mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 µL per well of S... View More

Product Image Gallery

IL-6 Antibody — Figure 7. Effect of Res treatment on miR-9, NF-kappaB1, IL-6, MMP-13, caspase-3 expression in mouse chondrocytes. The relative (A) miR-9 and (B) NF-kappaB1 mRNA expression levels were determined by RT-qPCR in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. (C) The relative NF-kappaB1, IL-6, MMP-13 and caspase-3 protein expression levels were determined by western blot analysis in chondrocytes following treatment with 0, 15 or 30 mM Res for 48 h. Quantification of (D) NF-kappaB1, (E) IL-6, (F) MMP-13 and (G) caspase-3 protein expression. n=3. *P<0.05 vs. control group. miR, microRNA; NF-kappaB1, nuclear factor kappa B subunit 1; IL-6, interleukin-6; MMP-13, matrix metallopeptidase 13; Res, Resveratrol. {TM}

Product Details

M621B

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7IL6

Immunogen

Recombinant human IL-6.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223577

Product Specific Information

M621B targets IL-6 in ELISA and WB applications and shows reactivity with Human samples.

The M621B immunogen is recombinant human IL-6.

M621B detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

The M621B IL6 antibody (biotinylated conjugate of clone 7IL6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M620 (clone 5IL6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M621B (clone 7IL6) and antibody M620 (clone 5IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long non-coding RNA MEG3 promotes tumor necrosis factor-alpha induced oxidative stress and apoptosis in interstitial cells of cajal via targeting the microRNA-21 /I-kappa-B-kinase beta axis.

Bioengineered

Li J,Bai J,Tuerdi N,Liu K

Published figure using IL-6 monoclonal antibody (Product # M621B) in Western Blot

Fri Apr 01 00:00:00 EDT 2022

Long non-coding RNA MEG3 promotes tumor necrosis factor-alpha induced oxidative stress and apoptosis in interstitial cells of cajal via targeting the microRNA-21 /I-kappa-B-kinase beta axis.

Bioengineered

Li J,Bai J,Tuerdi N,Liu K

Published figure using IL-6 monoclonal antibody (Product # M621B) in Western Blot

Fri Apr 01 00:00:00 EDT 2022

Therapeutic effect of Resveratrol in the treatment of osteoarthritis via the MALAT1/miR-9/NF-κB signaling pathway.

Experimental and therapeutic medicine

Zhang G,Zhang H,You W,Tang X,Li X,Gong Z

Published figure using IL-6 monoclonal antibody (Product # M621B) in Western Blot

Sun Mar 01 00:00:00 EST 2020

Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.

Frontiers in immunology

Siebert JN,Hamann L,Verolet CM,Gameiro C,Grillet S,Siegrist CA,Posfay-Barbe KM

M621B was used in an ELISA assay to asses whether a defect somewhere along this toll-like receptor signally pathways increases susceptibility to recurrent pneumococcal low respiratory tract infections.

Mon Sep 16 00:00:00 EDT 2019

Kaposi's sarcoma-associated herpesvirus kaposin B induces unique monophosphorylation of STAT3 at serine 727 and MK2-mediated inactivation of the STAT3 transcriptional repressor TRIM28.

Journal of virology

King CA

Published figure using IL-6 monoclonal antibody (Product # M621B) in ELISA

Thu Aug 01 00:00:00 EDT 2013

Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.

Neurobiology of aging

Loewenbrueck KF,Tigno-Aranjuez JT,Boehm BO,Lehmann PV,Tary-Lehmann M

M621B was used in ELISA to study the role of Th1 responses to beta-amyloid in Down syndrome and Alzheimer disease

Fri Oct 01 00:00:00 EDT 2010

Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination.

Vaccine

Lawson S,Lunney J,Zuckermann F,Osorio F,Nelson E,Welbon C,Clement T,Fang Y,Wong S,Kulas K,Christopher-Hennings J

M621B was used in ELISA to develop a multiplex Luminex assay to measure pig cytokines for vaccine development

Mon Jul 19 00:00:00 EDT 2010

Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?

Arthritis and rheumatism

Page TH,Brown A,Timms EM,Foxwell BM,Ray KP

M621B was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes

Mon Nov 01 00:00:00 EDT 2010

A component of polysaccharide peptidoglycan complex on Lactobacillus induced an improvement of murine model of inflammatory bowel disease and colitis-associated cancer.

Immunology

Matsumoto S,Hara T,Nagaoka M,Mike A,Mitsuyama K,Sako T,Yamamoto M,Kado S,Takada T

M621B was used in ELISA to investigate the role of Lactobacillus polysaccharide peptidoglycan complex in chronic intestinal inflammatory disorders

Tue Sep 01 00:00:00 EDT 2009

Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Blood

Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS

M621B was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells

Sun Jun 15 00:00:00 EDT 2008

IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Journal of immunology (Baltimore, Md. : 1950)

Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS

M621B was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

Thu Feb 15 00:00:00 EST 2007

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M621B was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 EDT 2006

Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells.

Clinical and experimental immunology

Matsumoto S,Hara T,Hori T,Mitsuyama K,Nagaoka M,Tomiyasu N,Suzuki A,Sata M

M621B was used in ELISA to study the effect of Lactobacillus casei strain Shirota on the production of interleukin 6 in LPS-stimulated murine large intestinal lamina propria mononuclear cells

Wed Jun 01 00:00:00 EDT 2005

Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.

Rheumatology (Oxford, England)

Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M

M621B was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Sun Aug 01 00:00:00 EDT 2004

Tumor-infiltrating lymphocyte secretion of IL-6 antagonizes tumor-derived TGF-beta 1 and restores the lymphokine-activated killing activity.

Journal of immunology (Baltimore, Md. : 1950)

Hsiao YW,Liao KW,Hung SW,Chu RM

M621B was used in ELISA to investigate the function of interleukin-6 from tumor-infiltrating lymphocytes

Sun Feb 01 00:00:00 EST 2004

Interleukin-6 fused to an anti-idiotype antibody in a vaccine increases the specific humoral immune response against CA125+ (MUC-16) ovarian cancer.

Cancer research

Reinartz S,Hombach A,Köhler S,Schlebusch H,Wallwiener D,Abken H,Wagner U

M621B was used in western blot to study the capacity of IL-6 fused to an anti-idiotype antibody in a vaccine to increase the specific humoral immune response against CA125+ ovarian cancer

Sun Jun 15 00:00:00 EDT 2003

Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.

International archives of allergy and immunology

Tsunoda M,Litonjua AA,Kuniak MP,Weiss ST,Satoh T,Guevarra L,Tollerud DJ

M621B was used in ELISA to investigate the relationship between serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women

Sat Feb 01 00:00:00 EST 2003

A nonsteroidal glucocorticoid receptor antagonist.

Molecular endocrinology (Baltimore, Md.)

Miner JN,Tyree C,Hu J,Berger E,Marschke K,Nakane M,Coghlan MJ,Clemm D,Lane B,Rosen J

M621B was used in immunocytochemistry to characterize a potent nonsteroidal antagonist against glucocorticoid receptor

Wed Jan 01 00:00:00 EST 2003

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Experimental lung research

Suwa T,Hogg JC,Vincent R,Mukae H,Fujii T,van Eeden SF

M621B was used in ELISA to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors

Tue Jul 09 00:00:00 EDT 2002

Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.

Journal of virology

King CA,Marshall JS,Alshurafa H,Anderson R

M621B was used in ELISA to investigate the role of mast cells/basophils in dengue pathogenesis

Tue Aug 01 00:00:00 EDT 2000

Regulation of interleukin-12 by complement receptor 3 signaling.

The Journal of experimental medicine

Marth T,Kelsall BL

M621B was used in ELISA to assess the interferon-alpha production in peripheral blood mononuclear cell

Mon Jun 02 00:00:00 EDT 1997

Sustained high levels of circulating chaperoned interleukin-6 after active specific cancer immunotherapy.

Blood

May LT,Patel K,García D,Ndubuisi MI,Ferrone S,Mittelman A,Mackiewicz A,Sehgal PB

M621B was used in ELISA to investigate the changes of interleukin 6 level in patients with cancer immunotherapy

Thu Sep 15 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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Function(s)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

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IL-6 Monoclonal Antibody (7IL6), Biotin

IL-6 Antibody (M621B) in ELISA

Product Details

M621B

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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