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KCC2 Antibody (MA5-45496) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with KCC2 monoclonal antibody (Product # MA5-45496) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin sta... View More
Product Details
MA5-45496
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N1/12
Immunogen
Fusion protein amino acids 932-1043 corresponding to rat KCC2
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931950
Product Specific Information
1 µg/mL of MA5-45496 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa.
This antibody was formerly sold as clone S1-12.
Target Information
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; Furosemide-sensitive K-Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5; solute carrier family 12, member 5
Gene Aliases: EIEE34; EIG14; hKCC2; KCC2; KIAA1176; mKIAA1176; SLC12A5
UniProt ID: (Human) Q9H2X9, (Mouse) A2A5L0, (Rat) Q63633
Entrez Gene ID: (Human) 57468, (Mouse) 57138, (Rat) 171373
transport
ion transport
cellular ion homeostasis
chemical synaptic transmission
learning
cellular chloride ion homeostasis
multicellular organism growth
thermosensory behavior
response to drug
dendritic spine development
potassium ion transmembrane transport
chloride transmembrane transport
potassium ion transport
chloride transport
organic cation transport
ammonium transport
intracellular pH reduction
transmembrane transport
ammonium transmembrane transport
cation transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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