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KCC4 Antibody (PA5-88472) in WB
Western blot analysis of KCC4 in extracts of various cell lines. Samples were incubated with KCC4 Polyclonal antibody (Product # PA5-88472) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-88472
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human SLC12A7 (NP_0065892)
Conjugate
Form
Liquid
Concentration
1.02 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804934
Product Specific Information
Immunogen sequence: MPTNFTVVPV EAHADGGGDE TAERTEAPGT PEGPEPERPS PGDGNPRENS PFLNNVEVEQ ESFFEGKNMA; Positive Samples: Mouse kidney, Mouse liver, Rat kidney; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May mediate K(+) uptake into Deiters' cells in the cochlea and contribute to K(+) recycling in the inner ear. Important for the survival of cochlear outer and inner hair cells and the maintenance of the organ of Corti. May be required for basolateral Cl(-) extrusion in the kidney and contribute to renal acidification. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Electroneutral potassium-chloride cotransporter 4; furosemide-sensitive KCl cotransporter 4; K-Cl cotransporter 4; K-Cl cotransporter KCC4; slc12a7 {ECO:0000312|EMBL:AAH86339.1, ECO:0000312|RGD:1359672}; solute carrier family 12 (potassium/chloride transporters), member 7; Solute carrier family 12 member 7
Gene Aliases: AA408796; D13Ertd261e; Kcc4; Slc12a7
UniProt ID: (Rat) Q5RK27, (Mouse) Q9WVL3
Entrez Gene ID: (Rat) 308069, (Mouse) 20499
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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