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KChIP3 Antibody (PA5-114936) in ICC/IF
Immunocytochemistry analysis of KChIP3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with KChIP3 polyclonal antibody (Product # PA5-114936) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-114936
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human KCNIP3(Accession Q9Y2W7), corresponding to amino acid residues R39-T89.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899572
Product Specific Information
Antibody detects endogenous levels of total Calsenilin/KCNIP3.
Target Information
Voltage-gated potassium (Kv) channels are key components of the potassium currents in the heart and central nervous system. In the heart, Kv4 channels are involved in the repolarization phase of the action potential. In the brain however, these channels prevent reverse-propagation of action potentials. Found associated with Kv4 channels are a group of calcium-binding proteins termed KChIPs (Kv channel interacting proteins). KChIPs are small molecular weight proteins that bind to the cytoplasmic amino termini of Kv4 alpha-subunits and help to modulate its function. There are currently three known KChIPs; KChIP1 expressed in the brain; KChIP2 expressed in the brain, heart, and lung, and has three isoforms, a, b, and c; and KChIP3, also known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator) which is expressed in the brain and testis. KChIP3 has activities beyond the modulation of Kv4 currents. It has been shown that KChIP3 binds to the regulatory DRE and represses transcription from the early-response gene c-fos. Thus, KCHiP3 is the first known calcium-binding protein to function as a DNA-binding transcriptional regulator. KChIP3 is also found associated with the presenilins and is implicated in the mediation of beta-amyloid formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-type potassium channel modulatory protein 3; Calsenilin; calsenilin, presenilin binding protein, EF hand transcription factor; calsenilin, presenilin-binding protein, EF hand transcription factor; DRE-antagonist modulator; DREAM; KChIP3; Kv channel interacting protein 3; Kv channel interacting protein 3, calsenilin; Kv channel-interacting protein 3; potassium channel interacting protein 3
Gene Aliases: 4933407H12Rik; AI413860; CSEN; DREAM; KCHIP3; KCNIP3; R74849
UniProt ID: (Human) Q3YAC4, (Mouse) Q9QXT8
Entrez Gene ID: (Human) 30818, (Mouse) 56461
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription corepressor activity
voltage-gated ion channel activity
potassium channel activity
calcium ion binding
protein binding
potassium channel regulator activity
ion channel binding
protein C-terminus binding
sequence-specific DNA binding
metal ion binding
calcium-dependent protein binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transport
ion transport
potassium ion transport
intracellular protein transport
apoptotic process
sensory perception of pain
regulation of ion transmembrane transport
regulation of neuron apoptotic process
negative regulation of transcription, DNA-templated
response to pain
behavioral response to pain
potassium ion transmembrane transport
protein localization to plasma membrane
regulation of potassium ion transmembrane transport
regulation of transcription from RNA polymerase II promoter
signal transduction
cardiac conduction
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