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KChIP3 Antibody (PA5-80750) in WB
Western Blot using KChIP3 Polyclonal Antibody (Product # PA5-80750) at 1:500 dilution. Lane A: Mouse heart Tissue Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 36 kDa.
Product Details
PA5-80750
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human CSEN/calsenilin/KCNIP3 Protein (Met1-Ile256)
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788032
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human CSEN/calsenilin/KCNIP3.
Target Information
Voltage-gated potassium (Kv) channels are key components of the potassium currents in the heart and central nervous system. In the heart, Kv4 channels are involved in the repolarization phase of the action potential. In the brain however, these channels prevent reverse-propagation of action potentials. Found associated with Kv4 channels are a group of calcium-binding proteins termed KChIPs (Kv channel interacting proteins). KChIPs are small molecular weight proteins that bind to the cytoplasmic amino termini of Kv4 alpha-subunits and help to modulate its function. There are currently three known KChIPs; KChIP1 expressed in the brain; KChIP2 expressed in the brain, heart, and lung, and has three isoforms, a, b, and c; and KChIP3, also known as calsenilin and DREAM (downstream regulatory elements (DRE)-antagonist modulator) which is expressed in the brain and testis. KChIP3 has activities beyond the modulation of Kv4 currents. It has been shown that KChIP3 binds to the regulatory DRE and represses transcription from the early-response gene c-fos. Thus, KCHiP3 is the first known calcium-binding protein to function as a DNA-binding transcriptional regulator. KChIP3 is also found associated with the presenilins and is implicated in the mediation of beta-amyloid formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-type potassium channel modulatory protein 3; Calsenilin; calsenilin, presenilin-binding protein, EF hand transcription factor; DRE-antagonist modulator; DREAM; KChIP3; Kv channel interacting protein 3; Kv channel interacting protein 3, calsenilin; Kv channel-interacting protein 3; potassium channel interacting protein 3
Gene Aliases: CSEN; DREAM; KCHIP3; KCNIP3
UniProt ID: (Human) Q3YAC4
Entrez Gene ID: (Human) 30818
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription corepressor activity
voltage-gated ion channel activity
potassium channel activity
calcium ion binding
protein binding
potassium channel regulator activity
ion channel binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
intracellular protein transport
apoptotic process
signal transduction
cardiac conduction
potassium ion transmembrane transport
protein localization to plasma membrane
regulation of potassium ion transmembrane transport
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