Invitrogen

MX1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

5 References

View all (29) MX1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

MX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

MX1 Antibody (PA5-22101) in ICC/IF

Immunofluorescence analysis of MX1 was performed using 70% confluent log phase HeLa and HeLa cells treated with IFN Alpha (10 ng/mL, 16hrs). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MX1 Polyclonal Antibody (Product # PA5-22101) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More

MX1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MX1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MX1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of MX1 using MX1 Monoclonal Antibody (Product # PA5-22101) shows increased expression of MX1 protein in cytoplasm of HeLa cell line upon treatment with IFN Alpha. {TM}

Product Details

PA5-22101

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Bovine, Human, Non-human primate, Rabbit

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 194 and 475 (P20591) of MX1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.52 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11153215

Product Specific Information

Recommended positive controls: Jurkat, Raji, NCI-H929, human MX1-transfected 293T.

Predicted reactivity: Rhesus Monkey (92%), Chimpanzee (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that participates in the cellular antiviral response. The encoded protein is induced by type I and type II interferons and antagonizes the replication process of several different RNA and DNA viruses. There is a related gene located adjacent to this gene on chromosome 21, and there are multiple pseudogenes located in a cluster on chromosome 4. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Subviral Dense Bodies of Human Cytomegalovirus Enhance Interferon-Beta Responses in Infected Cells and Impair Progeny Production.

Viruses

Penner I,Büscher N,Krauter S,Plachter B

Published figure using MX1 polyclonal antibody (Product # PA5-22101) in Western Blot

Wed Jun 07 00:00:00 EDT 2023

The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses.

EMBO reports

Bonaventure B,Rebendenne A,Chaves Valadão AL,Arnaud-Arnould M,Gracias S,Garcia de Gracia F,McKellar J,Labaronne E,Tauziet M,Vivet-Boudou V,Bernard E,Briant L,Gros N,Djilli W,Courgnaud V,Parrinello H,Rialle S,Blaise M,Lacroix L,Lavigne M,Paillart JC,Ricci EP,Schulz R,Jouvenet N,Moncorgé O,Goujon C

Published figure using MX1 polyclonal antibody (Product # PA5-22101) in Western Blot

Mon Nov 07 00:00:00 EST 2022

System-wide identification of myeloid markers of TB disease and HIV-induced reactivation in the macaque model of Mtb infection and Mtb/SIV co-infection.

Frontiers in immunology

Gough M,Singh DK,Singh B,Kaushal D,Mehra S

Published figure using MX1 polyclonal antibody (Product # PA5-22101) in Immunohistochemistry

Tue Oct 25 00:00:00 EDT 2022

Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.

Cells

Penner I,Büscher N,Dejung M,Freiwald A,Butter F,Plachter B

PA5-22101 was used in Western Blotting to investigate the impact of dense bodies on cells, in the absence of a viral infection.

Tue Dec 13 00:00:00 EST 2022

Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon Response.

Cells

Penner I,Büscher N,Dejung M,Freiwald A,Butter F,Plachter B

PA5-22101 was used in Western Blotting to investigate the impact of dense bodies on cells, in the absence of a viral infection.

Tue Dec 13 00:00:00 EST 2022

SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells.

Journal of virology

Rebendenne A,Valadão ALC,Tauziet M,Maarifi G,Bonaventure B,McKellar J,Planès R,Nisole S,Arnaud-Arnould M,Moncorgé O,Goujon C

PA5-22101 was used in Western Blot to characterize in depth host cell responses against SARS-CoV-2 in primary human airway epithelia (HAE) and immortalized cell lines.

Thu Mar 25 00:00:00 EDT 2021

SARS-CoV-2 triggers an MDA-5-dependent interferon response which is unable to control replication in lung epithelial cells.

Journal of virology

Rebendenne A,Valadão ALC,Tauziet M,Maarifi G,Bonaventure B,McKellar J,Planès R,Nisole S,Arnaud-Arnould M,Moncorgé O,Goujon C

PA5-22101 was used in Western Blot to characterize in depth host cell responses against SARS-CoV-2 in primary human airway epithelia (HAE) and immortalized cell lines.

Thu Mar 25 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: GTP-binding protein; IFI-78K; Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78; interferon-inducible myxovirus resistance-1 protein; interferon-inducible protein p78; Interferon-regulated resistance GTP-binding protein MxA; MX dynamin-like GTPase 1; Myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78; Myxovirus resistance protein 1

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Gene Aliases: IFI-78K; IFI78; MX; MX1; Mx1-b; Mx1B; MxA

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UniProt ID: (Human) P20591, (Bovine) P79135

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Entrez Gene ID: (Human) 4599, (Bovine) 280872, (Rabbit) 100359014

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Function(s)

GTPase activity protein binding GTP binding identical protein binding membrane traffic protein

Process(es)

apoptotic process defense response signal transduction response to virus response to type I interferon negative regulation of viral genome replication innate immune response defense response to virus type I interferon signaling pathway response to type III interferon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MX1 Polyclonal Antibody

MX1 Antibody (PA5-22101) in ICC/IF

Product Details

PA5-22101

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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