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Invitrogen
N-terminal Arginylation Monoclonal Antibody (4A9), PE
FIGURE: 1 / 2
N-terminal Arginylation Antibody (MA5-45373) in Flow
Flow Cytometry analysis using human neuroblastoma cells. Fixation involved 90% Methanol. Samples were incubated with N-terminal Arginylation monoclonal antibody (Product # MA5-45373) at 1:50 for 30 min on ice, followed by Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42°C for 2 hours.
Product Details
MA5-45373
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4A9
Immunogen
Synthetic N-terminal arginylated peptide conjugated to KLH
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931827
Product Specific Information
A 1:1,000 dilution of MA5-45373 was sufficient for detection of N-terminal Arginylation in 0.5 µg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
Target Information
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: N terminal Arginine; N-terminal Arginine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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