Invitrogen

NEFL Monoclonal Antibody (DA2)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

8 References

View all (78) NEFL antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

NEFL Antibody in Immunocytochemistry (ICC/IF)

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NEFL Antibody (MA1-2010) in ICC/IF

Immunofluorescence analysis of Neurofilament, Light chain was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurofilament, Light chain (DA2) Mouse Monoclonal Antibody (Product # MA1-2010) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa ... View More

NEFL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NEFL Antibody in Immunohistochemistry (IHC)

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NEFL Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of NEFL was observed in PC-12 differentiated to neurons in comparison to PC-12 undifferentiated cells. Relative expression was also observed in Mouse Cerebellum and Mouse Brain in comparison to Mouse Heart and Mouse Lungs using Anti-NEFL Mouse Monoclonal Antibody (Product # MA1-2010) in Western Blot. {RE}

Product Details

MA1-2010

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

2-3 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Chicken, Human, Mouse

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Clone

DA2

Immunogen

Enzymatically dephosphorylated porcine neurofilaments.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_347003

Product Specific Information

MA1-2010 detects the neurofilament, light chain in human, rat, and mouse samples.

MA1-2010 has been successfully used in western blot, immunohistochemistry, and immunofluorescence. By western blot this antibody detects a ~70 kDa protein representing the neurofilament, light chain in mouse brain tissue lysate. In immunohistochemistry procedures MA1-2010 recognizes the neurofilament, light chain in mouse brain tissue. In immunofluorescence procedures MA1-2010 recognizes the neurofilament, light chain in rat cerebral cortices.

The MA1-2010 immunogen is enzymatically dephosphorylated porcine neurofilaments.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MiR-146a in ALS: Contribution to Early Peripheral Nerve Degeneration and Relevance as Disease Biomarker.

International journal of molecular sciences

Giagnorio E,Malacarne C,Cavalcante P,Scandiffio L,Cattaneo M,Pensato V,Gellera C,Riva N,Quattrini A,Dalla Bella E,Lauria G,Mantegazza R,Bonanno S,Marcuzzo S

Published figure using NEFL monoclonal antibody (Product # MA1-2010) in Western Blot

Mon Feb 27 00:00:00 EST 2023

Activation of neuronal Ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and axonal plasticity in mice.

Journal of neurochemistry

Bu F,Munshi Y,Furr JW,Min JW,Qi L,Patrizz A,Spahr ZR,Urayama A,Kofler JK,McCullough LD,Li J

MA1-2010 was used in Immunohistochemistry to identify a neuronal Rac1 signaling in improving functional recovery and axonal regeneration after stroke, suggesting a potential therapeutic target in the recovery stage of stroke.

Sat May 01 00:00:00 EDT 2021

Increased plasma neurofilament light chain concentration correlates with severity of post-mortem neurofibrillary tangle pathology and neurodegeneration.

Acta neuropathologica communications

Ashton NJ,Leuzy A,Lim YM,Troakes C,Hortobágyi T,Höglund K,Aarsland D,Lovestone S,Schöll M,Blennow K,Zetterberg H,Hye A

MA1-2010 was used in Immunohistochemistry to examine the connection between blood neurofilament light chain and brain tissue pathology.

Wed Jan 09 00:00:00 EST 2019

Increased plasma neurofilament light chain concentration correlates with severity of post-mortem neurofibrillary tangle pathology and neurodegeneration.

Acta neuropathologica communications

Ashton NJ,Leuzy A,Lim YM,Troakes C,Hortobágyi T,Höglund K,Aarsland D,Lovestone S,Schöll M,Blennow K,Zetterberg H,Hye A

MA1-2010 was used in Immunohistochemistry to examine the connection between blood neurofilament light chain and brain tissue pathology.

Wed Jan 09 00:00:00 EST 2019

Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture.

Frontiers in neuroscience

Lukiw WJ,Cong L,Jaber V,Zhao Y

MA1-2010 was used in Western Blotting to characterise a neuron-specific effect of a human gastrointestinal-tract microbiome-derived neurotoxin on decreased neurofilament light abundance in both sporadic Alzheimer's disease temporal lobe neocortex in vivo and in lipopolysaccharide-stressed HNG cells in vitro.

Tue Sep 29 00:00:00 EDT 2020

Microbiome-Derived Lipopolysaccharide (LPS) Selectively Inhibits Neurofilament Light Chain (NF-L) Gene Expression in Human Neuronal-Glial (HNG) Cells in Primary Culture.

Frontiers in neuroscience

Lukiw WJ,Cong L,Jaber V,Zhao Y

Tue Sep 29 00:00:00 EDT 2020

Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis.

Glia

Huizinga R,van der Star BJ,Kipp M,Jong R,Gerritsen W,Clarner T,Puentes F,Dijkstra CD,van der Valk P,Amor S

MA1-2010 was used in immunohistochemistry to investigate phagocytosis of neuronal antigens in a multiple sclerosis model.

Thu Mar 01 00:00:00 EST 2012

Alphab-crystallin is a target for adaptive immune responses and a trigger of innate responses in preactive multiple sclerosis lesions.

Journal of neuropathology and experimental neurology

van Noort JM,Bsibsi M,Gerritsen WH,van der Valk P,Bajramovic JJ,Steinman L,Amor S

MA1-2010 was used in western blot to compare the serum immunoglobulin G reactivity profiles obtained using the full spectrum of human myelin-associated proteins of multiple sclerosis patients and healthy control subjects.

Thu Jul 01 00:00:00 EDT 2010

Numerous conglomerate inclusions in slowly progressive familial amyotrophic lateral sclerosis with posterior column involvement.

Journal of the neurological sciences

Katayama S,Watanabe C,Noda K,Ohishi H,Yamamura Y,Nishisaka T,Inai K,Asayama K,Murayama S,Nakamura S

MA1-2010 was used in immunohistochemistry (paraffin) to characterize disease in a patient with familial amyotrophic lateral sclerosis.

Wed Dec 01 00:00:00 EST 1999

Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system.

Journal of neuroscience research

Edson KJ,Linck RW,Letourneau PC

MA1-2010 was used in immunocytochemistry to investigate the association between tektin-like protein and neurofilaments in chicken neurons

Sun Sep 01 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: 68 kDa neurofilament protein; FLJ53642; light molecular weight neurofilament protein; NEF3; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament, light polypeptide 68kDa; neurofilament-l; neurofilament-light; NF-L; NFM; NLC; OTTHUMP00000196614; protein phosphatase 1, regulatory subunit 110

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Gene Aliases: AI847934; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110

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UniProt ID: (Human) P07196, (Mouse) P08551, (Rat) P19527

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Entrez Gene ID: (Human) 4747, (Pig) 100521224, (Mouse) 18039, (Rat) 83613

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Function(s)

Ras guanyl-nucleotide exchange factor activity structural constituent of cytoskeleton protein binding protein C-terminus binding protein domain specific binding protein binding, bridging identical protein binding phospholipase binding protein heterodimerization activity structural molecule activity

Process(es)

MAPK cascade microtubule cytoskeleton organization anterograde axonal transport retrograde axonal transport response to toxic substance peripheral nervous system axon regeneration axonal transport of mitochondrion spinal cord development hippocampus development cerebral cortex development regulation of axon diameter neurofilament bundle assembly locomotion response to peptide hormone negative regulation of neuron apoptotic process positive regulation of GTPase activity intermediate filament polymerization or depolymerization intermediate filament organization neuron projection morphogenesis positive regulation of axonogenesis neuromuscular process controlling balance protein polymerization response to corticosterone response to sodium arsenite response to acrylamide axon development intermediate filament bundle assembly neurofilament cytoskeleton organization response to organic substance response to organonitrogen compound

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NEFL Monoclonal Antibody (DA2)

NEFL Antibody (MA1-2010) in ICC/IF

Product Details

MA1-2010

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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