Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 8
NOX2 Antibody (PA5-141135) in ICC/IF
Immunocytochemistry analysis of NOX2/gp91phox in HepG2 cells (green). Samples were incubated in NOX2/gp91phox Polyclonal antibody (Product # PA5-141135). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Product Details
PA5-141135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to C-terminal human NOX2/gp91phox.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932587
Target Information
Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase complex expresses in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Mox1 and gp91 contain identical C-terminal sequence identity, yet possess distinct expression patterns. gp91-phox expresses in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is predominantly detected in the colon, with low expression in the uterus and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 beta polypeptide; Cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP911; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; p91PHOX; Superoxide-generating NADPH oxidase heavy chain subunit
Gene Aliases: AMCBX2; C88302; CGD; CGD91-phox; CYBB; Cyd; GP91-1; GP91-PHOX; GP91PHOX; IMD34; NOX2; p91-PHOX
UniProt ID: (Human) P04839, (Mouse) Q61093
Entrez Gene ID: (Human) 1536, (Mouse) 13058
voltage-gated ion channel activity
protein binding
electron carrier activity
superoxide-generating NADPH oxidase activity
heme binding
metal ion binding
protein heterodimerization activity
flavin adenine dinucleotide binding
N-ethylmaleimide reductase activity
oxidoreductase activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
epoxyqueuosine reductase activity
oxidase
response to reactive oxygen species
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
superoxide metabolic process
ion transport
inflammatory response
regulation of ion transmembrane transport
superoxide anion generation
innate immune response
cell redox homeostasis
respiratory burst
vascular endothelial growth factor receptor signaling pathway
hydrogen peroxide biosynthetic process
oxidation-reduction process
transport
response to drug
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support