Invitrogen

NQO1 Monoclonal Antibody (A180)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5 Published Figures

10 References

View all (38) NQO1 antibodies

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Protocols

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Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

NQO1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

NQO1 Antibody (39-3700) in ICC/IF

Immunofluorescence analysis of NQO1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NQO1 (A180) Mouse Monoclonal Antibody (Product # 39-3700) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175... View More

Product Image Gallery

NQO1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of NQO1 was observed in HepG2 , HeLa , A549 , U-87 MG and SH-SY5Y in comparison to MOLT-4 and HEK-293 using NQO1 Monoclonal Antibody (A180), (Product # 39-3700) in Western Blot. {RE}

Product Details

39-3700

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A180

Immunogen

Recombinant human NQO1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533410

Target Information

NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions.

Cell death & disease

Eichler M,Distler U,Nasrullah U,Krishnan A,Kaulich M,Husnjak K,Eberhardt W,Rajalingam K,Tenzer S,Pfeilschifter J,Imre G

Published figure using NQO1 monoclonal antibody (Product # 39-3700) in Western Blot

Wed Apr 20 00:00:00 EDT 2022

Astrocytic Nrf2 expression protects spinal cord from oxidative stress following spinal cord injury in a male mouse model.

Journal of neuroinflammation

Zhao W,Gasterich N,Clarner T,Voelz C,Behrens V,Beyer C,Fragoulis A,Zendedel A

39-3700 was used in Western Blotting to show Nrf2-ARE activation during primary phase of SCI. Furthermore, cell specific hyperactivation of Nrf2 was sufficient to protect the spinal cord against injury which indicate a promising therapeutic approach for SCI-treatment.

Mon Jun 06 00:00:00 EDT 2022

NRF2 Dysregulation in Hepatocellular Carcinoma and Ischemia: A Cohort Study and Laboratory Investigation.

Radiology

Ziv E,Zhang Y,Kelly L,Nikolovski I,Boas FE,Erinjeri JP,Cai L,Petre EN,Brody LA,Covey AM,Getrajdman G,Harding JJ,Sofocleous C,Abou-Alfa GK,Solomon SB,Brown KT,Yarmohammadi H

39-3700 was used in Western Blot to test whether ischemia resistance is present in individuals with NRF2-mutated HCC and if this resistance can be overcome by means of NRF2 inhibition in HCC cell lines.

Thu Oct 01 00:00:00 EDT 2020

A ruthenium(II)-curcumin compound modulates NRF2 expression balancing the cancer cell death/survival outcome according to p53 status.

Journal of experimental & clinical cancer research : CR

Garufi A,Baldari S,Pettinari R,Gilardini Montani MS,D'Orazi V,Pistritto G,Crispini A,Giorno E,Toietta G,Marchetti F,Cirone M,D'Orazi G

39-3700 was used in Western Blotting to evaluate NRF2 and p53 in several cancer cell lines carrying different endogenous p53 status, using a novel curcumin compound since curcumin has been shown to target both NRF2 and p53 and have anti-tumor activity.

Tue Jun 30 00:00:00 EDT 2020

A ruthenium(II)-curcumin compound modulates NRF2 expression balancing the cancer cell death/survival outcome according to p53 status.

Journal of experimental & clinical cancer research : CR

Garufi A,Baldari S,Pettinari R,Gilardini Montani MS,D'Orazi V,Pistritto G,Crispini A,Giorno E,Toietta G,Marchetti F,Cirone M,D'Orazi G

Tue Jun 30 00:00:00 EDT 2020

Novel high throughput pooled shRNA screening identifies NQO1 as a potential drug target for host directed therapy for tuberculosis.

Scientific reports

Li Q,Karim AF,Ding X,Das B,Dobrowolski C,Gibson RM,Quiñones-Mateu ME,Karn J,Rojas RE

39-3700 was used in western blot to identify a potential drug target for host directed therapy for tuberculosis, NQO1, by a novel high throughput pooled shRNA screening method

Tue Jun 14 00:00:00 EDT 2016

NQO1 inhibits proteasome-mediated degradation of HIF-1α.

Nature communications

Oh ET,Kim JW,Kim JM,Kim SJ,Lee JS,Hong SS,Goodwin J,Ruthenborg RJ,Jung MG,Lee HJ,Lee CH,Park ES,Kim C,Park HJ

393700 was used in western blot to describe a function for NQO1 in stabilizing HIF-1alpha

Wed Dec 14 00:00:00 EST 2016

Cross-regulations among NRFs and KEAP1 and effects of their silencing on arsenic-induced antioxidant response and cytotoxicity in human keratinocytes.

Environmental health perspectives

Zhao R,Hou Y,Zhang Q,Woods CG,Xue P,Fu J,Yarborough K,Guan D,Andersen ME,Pi J

39-3700 was used in western blot to study the regulatory interactions of NRF2, NRF, and KEAP and their role in the anti-oxidant responses of human keratinocytes to arsenic toxicity.

Sun Apr 01 00:00:00 EDT 2012

Prolonged inorganic arsenite exposure suppresses insulin-stimulated AKT S473 phosphorylation and glucose uptake in 3T3-L1 adipocytes: involvement of the adaptive antioxidant response.

Biochemical and biophysical research communications

Xue P,Hou Y,Zhang Q,Woods CG,Yarborough K,Liu H,Sun G,Andersen ME,Pi J

39-3700 was used in western blot to report that arsenic exposure activates the adaptive oxidative stress response and impairs insulin-stimulated ROS signaling, and thus causes insulin resistance in adipocytes.

Fri Apr 08 00:00:00 EDT 2011

Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems.

The Journal of biological chemistry

Anwar A,Dehn D,Siegel D,Kepa JK,Tang LJ,Pietenpol JA,Ross D

Fri Mar 21 00:00:00 EST 2003

Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems.

The Journal of biological chemistry

Anwar A,Dehn D,Siegel D,Kepa JK,Tang LJ,Pietenpol JA,Ross D

Fri Mar 21 00:00:00 EST 2003

Bioinformatics

Protein Aliases: Azoreductase; Diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase 4; diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NQ01; Phylloquinone reductase; QR1; Quinone reductase 1

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Gene Aliases: DHQU; DIA4; DTD; NMOR1; NMORI; NQO1; QR1

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UniProt ID: (Human) P15559, (Rat) P05982

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Entrez Gene ID: (Dog) 610935, (Human) 1728, (Rat) 24314

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Function(s)

NAD(P)H dehydrogenase (quinone) activity cytochrome-b5 reductase activity, acting on NAD(P)H superoxide dismutase activity protein binding identical protein binding poly(A) RNA binding oxidoreductase

Process(es)

regulation of cellular amino acid metabolic process xenobiotic metabolic process nitric oxide biosynthetic process synaptic transmission, cholinergic aging response to nutrient response to toxic substance removal of superoxide radicals response to estradiol negative regulation of catalytic activity positive regulation of neuron apoptotic process response to ethanol oxidation-reduction process superoxide metabolic process response to oxidative stress response to organic cyclic compound

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NQO1 Monoclonal Antibody (A180)

NQO1 Antibody (39-3700) in ICC/IF

Product Details

39-3700

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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