Proteintech

PCNA Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (20)

PCNA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

PCNA Antibody (24036-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 24036-1-AP (PCNA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCNA Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

PCNA Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

24036-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PCNA Fusion Protein Ag7416 (8-256 aa encoded by BC000491)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody specifically react with the 36 kDa human PCNA protein.

Immunogen sequence: QGSILKKVL EALKDLINEA CWDISSSGVN LQSMDSSHVS LVQLTLRSEG FDTYRCDRNL AMGVNLTSMS KILKCAGNED IITLRAEDNA DTLALVFEAP NQEKVSDYEM KLMDLDVEQL GIPEQEYSCV VKMPSGEFAR ICRDLSHIGD AVVISCAKDG VKFSASGELG NGNIKLSQTS NVDKEEEAVT IEMNEPVQLT FALRYLNFFT KATPLSSTVT LSMSADVPLV VEYKIADMGH LKYYLAPKIE (8-256 aa encoded by BC000491)

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

U0126 and BAY11-7082 Inhibit the Progression of Endometriosis in a Rat Model by Suppressing the MEK/ERK/NF-κB Pathway.

Women's health reports (New Rochelle, N.Y.)

Wang F,Li YM,Li RY,Yang YE,Wei M,Ha C

Tue Mar 07 00:00:00 EST 2023

U0126 and BAY11-7082 Inhibit the Progression of Endometriosis in a Rat Model by Suppressing the MEK/ERK/NF-κB Pathway.

Women's health reports (New Rochelle, N.Y.)

Wang F,Li YM,Li RY,Yang YE,Wei M,Ha C

Tue Mar 07 00:00:00 EST 2023

Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression.

Oncology letters

Zhang J,Liu Y,Wang X,Wang Z,Xing E,Li J,Wang D

Sat Jul 01 00:00:00 EDT 2023

The LRP5 high-bone-mass mutation causes alveolar bone accrual with minor craniofacial alteration.

Journal of periodontal research

Turkkahraman H,Flanagan S,Zhu T,Bellido TM,Yuan X

Tue Aug 01 00:00:00 EDT 2023

Spontaneous neuronal regeneration in the forebrain of the leopard gecko (Eublepharis macularius) following neurochemical lesioning.

Developmental dynamics : an official publication of the American Association of Anatomists

Austin LE,Graham C,Vickaryous MK

Sun Jan 01 00:00:00 EST 2023

Spontaneous neuronal regeneration in the forebrain of the leopard gecko (Eublepharis macularius) following neurochemical lesioning.

Developmental dynamics : an official publication of the American Association of Anatomists

Austin LE,Graham C,Vickaryous MK

Sun Jan 01 00:00:00 EST 2023

Plasma exosomes confer hypoxic pulmonary hypertension by transferring LOX-1 cargo to trigger phenotypic switching of pulmonary artery smooth muscle cells.

Biochemical pharmacology

Huang N,Wang D,Zhu TT,Ge XY,Liu H,Yao MZ,Guo YZ,Peng J,Wang Q,Zhang Z,Hu CP

Sun Jan 01 00:00:00 EST 2023

Plasma exosomes confer hypoxic pulmonary hypertension by transferring LOX-1 cargo to trigger phenotypic switching of pulmonary artery smooth muscle cells.

Biochemical pharmacology

Huang N,Wang D,Zhu TT,Ge XY,Liu H,Yao MZ,Guo YZ,Peng J,Wang Q,Zhang Z,Hu CP

Sun Jan 01 00:00:00 EST 2023

Transcription factor Dmrt1 triggers the SPRY1-NF-κB pathway to maintain testicular immune homeostasis and male fertility.

Zoological research

Zhang MF,Wan SC,Chen WB,Yang DH,Liu WQ,Li BL,Aierken A,Du XM,Li YX,Wu WP,Yang XC,Wei YD,Li N,Peng S,Li XL,Li GP,Hua JL

Thu May 18 00:00:00 EDT 2023

Tendon Cells Root Into (Instead of Attach to) Humeral Bone Head via Fibrocartilage-Enthesis.

International journal of biological sciences

Wang Z,Ma C,Chen D,Haslett C,Xu C,Dong C,Wang X,Zheng M,Jing Y,Feng JQ

Wed Jan 04 00:00:00 EST 2023

Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells.

Oncology reports

Xing X,Liu M,Wang X,Guo Q,Wang H

Wed Feb 01 00:00:00 EST 2023

Promoting effects of calponin 3 on the growth of diffuse large B‑cell lymphoma cells.

Oncology reports

Xing X,Liu M,Wang X,Guo Q,Wang H

Wed Feb 01 00:00:00 EST 2023

Type 1 diabetes mellitus leads to gingivitis and an early compensatory increase in bone remodeling.

Journal of periodontology

Yuan X,Amin V,Zhu T,Kittaka M,Ueki Y,Bellido TM,Turkkahraman H

Wed Feb 01 00:00:00 EST 2023

Epigenetic silencing of JAM3 promotes esophageal cancer development by activating Wnt signaling.

Clinical epigenetics

Yang W,Guo C,Herman JG,Zhu C,Lv H,Su X,Zhang L,Zhang M,Guo M

Fri Dec 02 00:00:00 EST 2022

Engineered Exosomes-Mediated Transfer of hsa-miR-320a Overcomes Chemoresistance in Cervical Cancer Cells via Targeting MCL1.

Frontiers in pharmacology

Zhou J,Wang Y,Zhang L,Chen Q,Zhu X,Jiang P,Jiang N,Zhao W,Li B

Sat Jul 16 00:00:00 EDT 2022

Single-cell transcriptomics reveals variable trajectories of CSPCs in the progression of osteoarthritis.

Heliyon

Qi L,Wang J,Chen X,Ding Y,Ling B,Wang W,Xu J,Xue Z

Tue Nov 01 00:00:00 EDT 2022

Single-cell transcriptomics reveals variable trajectories of CSPCs in the progression of osteoarthritis.

Heliyon

Qi L,Wang J,Chen X,Ding Y,Ling B,Wang W,Xu J,Xue Z

Tue Nov 01 00:00:00 EDT 2022

Fused placentas: Till birth do us part.

Placenta

Lopez-Tello J,Sferruzzi-Perri AN

Fri Jan 01 00:00:00 EST 2021

Hepatocyte Growth Factor Overexpression Slows the Progression of 4NQO-Induced Oral Tumorigenesis.

Frontiers in oncology

He X,Chen S,Tang Y,Zhao X,Yan L,Wu L,Wu Z,Liu W,Chen X,Wang X

Fri Apr 29 00:00:00 EDT 2022

Hydroxytyrosol Inhibits MDSCs and Promotes M1 Macrophages in Mice With Orthotopic Pancreatic Tumor.

Frontiers in pharmacology

Wang B,Yang L,Liu T,Xun J,Zhuo Y,Zhang L,Zhang Q,Wang X

Sat Dec 04 00:00:00 EST 2021

Tunicamycin enhances the suppressive effects of cisplatin on lung cancer growth through PTX3 glycosylation via AKT/NF-κB signaling pathway.

International journal of oncology

Ahmmed B,Khan MN,Nisar MA,Kampo S,Zheng Q,Li Y,Yan Q

Fri Feb 01 00:00:00 EST 2019

Tunicamycin enhances the suppressive effects of cisplatin on lung cancer growth through PTX3 glycosylation via AKT/NF-κB signaling pathway.

International journal of oncology

Ahmmed B,Khan MN,Nisar MA,Kampo S,Zheng Q,Li Y,Yan Q

Fri Feb 01 00:00:00 EST 2019

S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Fang C,Bian G,Ren P,Xiang J,Song J,Yu C,Zhang Q,Liu L,Chen K,Liu F,Zhang K,Wu C,Sun R,Hu D,Ju G,Wang J

Sun Jul 01 00:00:00 EDT 2018

S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Fang C,Bian G,Ren P,Xiang J,Song J,Yu C,Zhang Q,Liu L,Chen K,Liu F,Zhang K,Wu C,Sun R,Hu D,Ju G,Wang J

Sun Jul 01 00:00:00 EDT 2018

Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles.

PloS one

Zhang S,Zheng D,Wu Y,Lin W,Chen Z,Meng L,Liu J,Zhou Y

Mon Aug 01 00:00:00 EDT 2016

Simulated Microgravity Using a Rotary Culture System Compromises the In Vitro Development of Mouse Preantral Follicles.

PloS one

Zhang S,Zheng D,Wu Y,Lin W,Chen Z,Meng L,Liu J,Zhou Y

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

View more View less

Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR

View more View less

UniProt ID: (Human) P12004, (Rat) P04961, (Mouse) P17918

View more View less

Entrez Gene ID: (Human) 5111, (Rat) 25737, (Mouse) 18538

View more View less

Function(s)

purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding transcription factor binding DNA binding DNA polymerase processivity factor

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication negative regulation of transcription from RNA polymerase II promoter regulation of DNA replication base-excision repair, gap-filling cellular response to xenobiotic stimulus DNA replication DNA repair cellular response to DNA damage stimulus regulation of catalytic activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PCNA Polyclonal Antibody

PCNA Antibody (24036-1-AP) in ICC/IF

Product Details

24036-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

RNA Immunoprecipitation (RIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!