Invitrogen

PCNA Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

8 Published Figures

18 References

View all (142) PCNA antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PCNA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

PCNA Antibody (PA5-27214) in ICC/IF

Immunofluorescence analysis of PCNA was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PCNA Polyclonal Antibody (Product # PA5-27214, 1:100 dilution) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:20... View More

PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCNA Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PCNA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PCNA siRNA and reduction of signal was observed in Western Blot using PCNA Polyclonal Antibody (Product # PA5-27214). {KD}

Product Details

PA5-27214

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.11 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544690

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, PC-12, A549, SiHa.

Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (92%), Xenopus laevis (89%), Pig (100%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDK4/6 inhibition confers protection of normal gut epithelia against gemcitabine and the active metabolite of irinotecan.

Cell cycle (Georgetown, Tex.)

Blume J,Claus L,Isermann T,Dickmanns A,Conradi LC,Schulz-Heddergott R,Dobbelstein M

Published figure using PCNA polyclonal antibody (Product # PA5-27214) in Western Blot

Sat Jul 01 00:00:00 EDT 2023

Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules.

International journal of molecular sciences

Isaeva EV,Beketov EE,Demyashkin GA,Yakovleva ND,Arguchinskaya NV,Kisel AA,Lagoda TS,Malakhov EP,Smirnova AN,Petriev VM,Eremin PS,Osidak EO,Domogatsky SP,Ivanov SA,Shegay PV,Kaprin AD

Published figure using PCNA polyclonal antibody (Product # PA5-27214) in Immunohistochemistry

Mon Feb 28 00:00:00 EST 2022

Murine Respiratory Tract Infection with Classical Klebsiella pneumoniae Induces Bronchus-Associated Lymphoid Tissue.

Infection and immunity

Wasbotten RK,Dahler AA,Mackel JJ,Morffy Smith C,Rosen DA

PA5-27214 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization, Western Blot to confirm that murine lungs infected with K. pneumoniae develop iBALT, which may play a role in pulmonary immunity to this troublesome pathogen.

Thu Apr 21 00:00:00 EDT 2022

Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy.

Andrologia

Feng C,Wang Q,Deng L,Peng N,Yang M,Wang X

PA5-27214 was used in Western Blotting to demonstrate that high circ_0074032 level in PCa is associated with worse PCa-related prognosis. Circ_0074032 induced cell apoptosis, curbed cell proliferation, migration and invasion in PCa cells and was identified as miR-198 decoy. Circ_0074032 regulates HOXA1 expression and promotes PCa progression via elevating HOXA1 expression through acting as a miR-198 sponge.

Tue Feb 01 00:00:00 EST 2022

On the Pathomorphological Pattern of the Efficiency of Photodynamic Therapy of Murine Melanoma B16 Using a New Photosensitizer Based on Chlorin e₆ Conjugate with a Prostate-Specific Membrane Antigen.

Molecules (Basel, Switzerland)

Abramova OB,Demyashkin GA,Drozhzhina VV,Yakovleva ND,Kozlovtseva EA,Sivovolova TP,Shegay PV,Ivanov SA,Kaprin AD

PA5-27214 was used in Immunohistochemistry to assess the antitumor efficacy of photodynamic therapy (PDT) with a novel photosensitizer (PS) in murine B16 melanoma, with a focus on evaluating the therapeutic potential of PDT through histological studies of irradiated zones on the 21st day post-treatment, demonstrating the effectiveness of PDT depended on PS dosage and laser irradiation parameters.

Thu May 26 00:00:00 EDT 2022

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using PCNA polyclonal antibody (Product # PA5-27214) in Western Blot

Fri Dec 31 00:00:00 EST 2021

Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma.

Journal of chemotherapy (Florence, Italy)

Li SL,Jiang TQ,Cao QW,Liu SM

Published figure using PCNA polyclonal antibody (Product # PA5-27214) in Western Blot

Mon Feb 01 00:00:00 EST 2021

Endothelial cell PHD2-HIF1α-PFKFB3 contributes to right ventricle vascular adaptation in pulmonary hypertension.

American journal of physiology. Lung cellular and molecular physiology

Kassa B,Kumar R,Mickael C,Sanders L,Vohwinkel C,Lee MH,Gu S,Poth JM,Stenmark KR,Zhao YY,Tuder RM,Graham BB

PA5-27214 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization, Western Blot to find no evidence for a contribution of circulating EC precursors to the RV vascular network.

Fri Oct 01 00:00:00 EDT 2021

Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma.

Oncology reports

An YW,Liu HQ,Zhou ZQ,Wang JC,Jiang GY,Li ZW,Wang F,Jin HT

PA5-27214 was used in Western Blotting to explore the antitumor effects of sinoporphyrin sodium (DVDMS)‚mediated photodynamic therapy (PDT) and sonodynamic therapy (SDT) in glioma, and to reveal the underlying mechanisms.

Thu Oct 01 00:00:00 EDT 2020

Protective Effects of Dietary Grape on UVB-Mediated Cutaneous Damages and Skin Tumorigenesis in SKH-1 Mice.

Cancers

Mintie CA,Musarra AK,Singh CK,Ndiaye MA,Sullivan R,Eickhoff JC,Ahmad N

PA5-27214 was used in Immunohistochemistry (Paraffin) to show dietary grape powder can protect against UVB-mediated skin damage and carcinogenesis in SKH-1 mice.

Wed Jul 01 00:00:00 EDT 2020

Protective Effects of Dietary Grape on UVB-Mediated Cutaneous Damages and Skin Tumorigenesis in SKH-1 Mice.

Cancers

Mintie CA,Musarra AK,Singh CK,Ndiaye MA,Sullivan R,Eickhoff JC,Ahmad N

PA5-27214 was used in Immunohistochemistry (Paraffin) to show dietary grape powder can protect against UVB-mediated skin damage and carcinogenesis in SKH-1 mice.

Wed Jul 01 00:00:00 EDT 2020

Nephropathy induced by renal microembolism: a characterization of biochemical and histopathological changes in rats.

International journal of clinical and experimental pathology

Bersani-Amado LE,da Rocha BA,Schneider LCL,Ames FQ,Breithaupt Faloppa AC,Araújo GB,Dantas JA,Bersani-Amado CA,Cuman RKN

PA5-27214 was used in Immunohistochemistry to investigate some biochemical parameters of renal function and the vascular, glomerular, tubular, and interstitial manifestations in the progression of nephropathy induced by renal microembolism.

Fri Jan 17 00:00:00 EST 2020

Protective effects of either C-peptide or l-arginine on pancreatic β-cell function, proliferation, and oxidative stress in streptozotocin-induced diabetic rats.

Journal of cellular physiology

Ragy MM,Ahmed SM

PA5-27214 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization, Western Blot to show that induction of diabetes causes hyperglycemia, dyslipidemia, and oxidative stress.

Mon Jul 01 00:00:00 EDT 2019

STAT3 deficiency prevents hepatocarcinogenesis and promotes biliary proliferation in thioacetamide-induced liver injury.

World journal of gastroenterology

Abe M,Yoshida T,Akiba J,Ikezono Y,Wada F,Masuda A,Sakaue T,Tanaka T,Iwamoto H,Nakamura T,Sata M,Koga H,Yoshimura A,Torimura T

Published figure using PCNA polyclonal antibody (Product # PA5-27214) in Immunohistochemistry

Sat Oct 07 00:00:00 EDT 2017

Effects of the aryl hydrocarbon receptor agonist 3-methylcholanthrene on the 17β-estradiol regulated mRNA transcriptome of the rat uterus.

The Journal of steroid biochemistry and molecular biology

Helle J,Keiler AM,Zierau O,Dörfelt P,Vollmer G,Lehmann L,Chittur SV,Tenniswood M,Welsh J,Kretzschmar G

PA527214 was used in immunohistochemistry - paraffin section to examine the cross talk between the aryl hydrocarbon receptor and the estrogen receptor on a mRNA transcriptome wide scale

Sat Jul 01 00:00:00 EDT 2017

PCNA is recruited to irradiated chromatin in late S-phase and is most pronounced in G2 phase of the cell cycle.

Protoplasma

Bártová E,Suchánková J,Legartová S,Malyšková B,Hornáček M,Skalníková M,Mašata M,Raška I,Kozubek S

PA527214 was used in immunocytochemistry to find that proliferating cell nuclear antigen plays a role in DNA repair in late S-phase and is maintained during the G2 phase of the cell cycle

Fri Sep 01 00:00:00 EDT 2017

Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats.

Toxicology mechanisms and methods

Hasan SK,Sultana S

PA5-27214 was used in immunohistochemistry - paraffin section to analyze attenuation of 2-acetylaminofluorene induced by oxidative stress, apoptosis and inflammation in the liver of wistar rats by geraniol

Tue Aug 02 00:00:00 EDT 2016

Therapeutic Touch Has Significant Effects on Mouse Breast Cancer Metastasis and Immune Responses but Not Primary Tumor Size.

Evidence-based complementary and alternative medicine : eCAM

Gronowicz G,Secor ER,Flynn JR,Jellison ER,Kuhn LT

PA5-27214 was used in immunohistochemistry - paraffin section to study the positive effects of therapeutic touch on mouse breast cancer metastasis and immune response, but not in primary tumor size

Fri Jun 26 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

View more View less

Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR

View more View less

UniProt ID: (Human) P12004, (Rat) P04961, (Chinese hamster) P57761, (Mouse) P17918

View more View less

Entrez Gene ID: (Human) 5111, (Rat) 25737, (Chinese hamster) 100689335, (Mouse) 18538

View more View less

Function(s)

purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding transcription factor binding DNA binding DNA polymerase processivity factor

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication negative regulation of transcription from RNA polymerase II promoter regulation of DNA replication base-excision repair, gap-filling cellular response to xenobiotic stimulus DNA replication DNA repair cellular response to DNA damage stimulus regulation of catalytic activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PCNA Polyclonal Antibody

PCNA Antibody (PA5-27214) in ICC/IF

Product Details

PA5-27214

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!