Invitrogen

PCNA Monoclonal Antibody (PC10), Biotin

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

14 References

View all (142) PCNA antibodies

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PCNA Antibody (13-3940) in WB

Western blot analysis of PCNA was performed by loading 20 µg of HeLa (lane1), Raji (lane2), MCF-7 (lane3), Jurkat (lane4), HEK-293 (lane5), NIH/3T3 (lane6), MDA-MB-231 (lane7) cell lysate using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. PCNA was detected at ... View More

PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PCNA Antibody in Immunocytochemistry (ICC/IF)

PCNA Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PCNA Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PCNA siRNA and loss of signal was observed in Western Blot using Anti-PCNA monoclonal Antibody (Product # 13-3940). {KD}

Product Details

13-3940

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Insect, Mouse, Non-human primate, Rat, Yeast

Published species

Human, Mouse, Sheep

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PC10

Immunogen

Rat recombinant PCNA polypeptides.

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533017

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1.

Journal of orthopaedic surgery and research

Zhang H,Wu G,Feng J,Lu X,Liu P

13-3940 was used in Immunohistochemistry (Paraffin), Western Blot to demonstrate that STOML2 silencing inhibits cell proliferation and glycolysis through downregulation of PAI-1 expression, suggesting a new therapeutic target for MM.

Sat Nov 13 00:00:00 EST 2021

HMGB1 amplifies ILC2-induced type-2 inflammation and airway smooth muscle remodelling.

PLoS pathogens

Loh Z,Simpson J,Ullah A,Zhang V,Gan WJ,Lynch JP,Werder RB,Sikder AA,Lane K,Sim CB,Porrello E,Mazzone SB,Sly PD,Steptoe RJ,Spann KM,Sukkar MB,Upham JW,Phipps S

13-3940 was used in Immunohistochemistry to investigate the processes that mediate tissue damage-induced type-2 inflammation and the origins of airway remodelling, using a preclinical mouse model of viral bronchiolitis.

Wed Jul 01 00:00:00 EDT 2020

The cJUN NH₂-terminal kinase (JNK) signaling pathway promotes genome stability and prevents tumor initiation.

eLife

Girnius N,Edwards YJ,Garlick DS,Davis RJ

13-3940 was used in Immunohistochemistry to identify cJUN NH2-terminal kinase pathway defects as 'driver' mutations that promote genome instability and tumor initiation.

Fri Jun 01 00:00:00 EDT 2018

JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins.

Cell reports

Girnius N,Davis RJ

13-3940 was used in Immunohistochemistry-immunofluorescence to study the role of the cJUN NH2-terminal kinase signalling pathway in anoikis.

Tue Nov 14 00:00:00 EST 2017

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture.

Nature medicine

Li X,Nadauld L,Ootani A,Corney DC,Pai RK,Gevaert O,Cantrell MA,Rack PG,Neal JT,Chan CW,Yeung T,Gong X,Yuan J,Wilhelmy J,Robine S,Attardi LD,Plevritis SK,Hung KE,Chen CZ,Ji HP,Kuo CJ

Published figure using PCNA monoclonal antibody (Product # 13-3940) in Immunofluorescence

Tue Jul 01 00:00:00 EDT 2014

Oncogenic transformation of diverse gastrointestinal tissues in primary organoid culture.

Nature medicine

Li X,Nadauld L,Ootani A,Corney DC,Pai RK,Gevaert O,Cantrell MA,Rack PG,Neal JT,Chan CW,Yeung T,Gong X,Yuan J,Wilhelmy J,Robine S,Attardi LD,Plevritis SK,Hung KE,Chen CZ,Ji HP,Kuo CJ

Published figure using PCNA monoclonal antibody (Product # 13-3940) in Immunofluorescence

Tue Jul 01 00:00:00 EDT 2014

The role of JNK in the development of hepatocellular carcinoma.

Genes & development

Das M,Garlick DS,Greiner DL,Davis RJ

Published figure using PCNA monoclonal antibody (Product # 13-3940) in Immunofluorescence

Tue Mar 15 00:00:00 EDT 2011

The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk.

Journal of immunology (Baltimore, Md. : 1950)

Sayi A,Kohler E,Hitzler I,Arnold I,Schwendener R,Rehrauer H,Müller A

13-3940 was used in immunohistochemistry - paraffin section to describe a dual role for IFN-gamma in Helicobacter pathogenesis

Mon Jun 01 00:00:00 EDT 2009

Induction of KLF4 in basal keratinocytes blocks the proliferation-differentiation switch and initiates squamous epithelial dysplasia.

Oncogene

Foster KW,Liu Z,Nail CD,Li X,Fitzgerald TJ,Bailey SK,Frost AR,Louro ID,Townes TM,Paterson AJ,Kudlow JE,Lobo-Ruppert SM,Ruppert JM

Thu Feb 24 00:00:00 EST 2005

Partial SP-B deficiency perturbs lung function and causes air space abnormalities.

American journal of physiology. Lung cellular and molecular physiology

Nesslein LL,Melton KR,Ikegami M,Na CL,Wert SE,Rice WR,Whitsett JA,Weaver TE

13-3940 was used in immunohistochemistry to use transgenic mice to investigate how surfactant protein B deficiency perturbs pulmonary function

Wed Jun 01 00:00:00 EDT 2005

Anticancer activity of docetaxel in murine salivary gland carcinoma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Piechocki MP,Lonardo F,Ensley JF,Nguyen T,Kim H,Yoo GH

13-3940 was used in immunohistochemistry (paraffin) to assess the effects of docetaxel on salivary gland carcinoma.

Fri Mar 01 00:00:00 EST 2002

Injury, inflammation, and remodeling in fetal sheep lung after intra-amniotic endotoxin.

American journal of physiology. Lung cellular and molecular physiology

Kramer BW,Kramer S,Ikegami M,Jobe AH

Thu Aug 01 00:00:00 EDT 2002

The 104-123 amino acid sequence of the beta-domain of von Hippel-Lindau gene product is sufficient to inhibit renal tumor growth and invasion.

Cancer research

Datta K,Sundberg C,Karumanchi SA,Mukhopadhyay D

Thu Mar 01 00:00:00 EST 2001

The 104-123 amino acid sequence of the beta-domain of von Hippel-Lindau gene product is sufficient to inhibit renal tumor growth and invasion.

Cancer research

Datta K,Sundberg C,Karumanchi SA,Mukhopadhyay D

Thu Mar 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen

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Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR; POL30; YBR0811; YBR088C

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UniProt ID: (Human) P12004, (Baker's yeast) D6VQ89, (Rat) P04961, (Mouse) P17918

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Entrez Gene ID: (Human) 5111, (Baker's yeast) 852385, (Rat) 25737, (Mouse) 18538

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Function(s)

purine-specific mismatch base pair DNA N-glycosylase activity chromatin binding damaged DNA binding protein binding enzyme binding estrogen receptor binding DNA polymerase processivity factor activity receptor tyrosine kinase binding dinucleotide insertion or deletion binding MutLalpha complex binding histone acetyltransferase binding identical protein binding DNA polymerase binding DNA binding transcription factor binding DNA polymerase processivity factor

Process(es)

G1/S transition of mitotic cell cycle regulation of transcription involved in G1/S transition of mitotic cell cycle telomere maintenance via recombination telomere maintenance DNA strand elongation involved in DNA replication leading strand elongation transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest heart development cell proliferation protein sumoylation translesion synthesis epithelial cell differentiation replication fork processing positive regulation of deoxyribonuclease activity nucleotide-excision repair, DNA incision response to lipid cellular response to UV error-prone translesion synthesis DNA damage response, detection of DNA damage positive regulation of DNA repair positive regulation of DNA replication response to cadmium ion error-free translesion synthesis mitotic telomere maintenance via semi-conservative replication mitotic cell cycle meiotic mismatch repair DNA replication lagging strand elongation regulation of DNA replication DNA repair nucleotide-excision repair postreplication repair cellular response to DNA damage stimulus mitotic sister chromatid cohesion chromatin silencing at silent mating-type cassette telomeric heterochromatin assembly establishment of mitotic sister chromatid cohesion maintenance of DNA trinucleotide repeats positive regulation of exodeoxyribonuclease activity positive regulation of phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands positive regulation of exonuclease activity negative regulation of transcription from RNA polymerase II promoter base-excision repair, gap-filling cellular response to xenobiotic stimulus regulation of catalytic activity

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PCNA Monoclonal Antibody (PC10), Biotin

PCNA Antibody (13-3940) in WB

Product Details

13-3940

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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