Invitrogen

PPAR gamma Monoclonal Antibody (K.242.9)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

18 References

View all (31) PPAR gamma antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PPAR gamma Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

PPAR gamma Antibody (MA5-14889) in ICC/IF

Immunofluorescent analysis of PPAR-gamma in 3T3-L1 cells using a PPAR-gamma monoclonal antibody (Product # MA5-14889) (red) showing nuclear localization in differentiated cells. Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.

PPAR gamma Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PPAR gamma Antibody in Western Blot (WB)

PPAR gamma Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-14889

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:1,600

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

K.242.9

Immunogen

Synthetic peptide corresponding to residues surrounding Asp69 of human PPAR-gamma

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

125 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10985650

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion of Spinophilin Promotes White Adipocyte Browning.

Pharmaceuticals (Basel, Switzerland)

Gou W,Wei H,Swaby L,Green E,Wang H

MA5-14889 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the critical role of SPL in the regulation of WAT browning.

Sun Jan 08 00:00:00 EST 2023

Deletion of Spinophilin Promotes White Adipocyte Browning.

Pharmaceuticals (Basel, Switzerland)

Gou W,Wei H,Swaby L,Green E,Wang H

MA5-14889 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate the critical role of SPL in the regulation of WAT browning.

Sun Jan 08 00:00:00 EST 2023

Hepatic PPARα Is Destabilized by SIRT1 Deacetylase in Undernourished Male Mice.

Frontiers in nutrition

Suh JH,Kim KH,Conner ME,Moore DD,Preidis GA

MA5-14889 was used in Immunoprecipitation to suggest that induction of SIRT1 in undernutrition results in hepatic PPARα deacetylation, ubiquitination, and degradation, highlighting a new mechanism that mediates the liver's failed adaptive metabolic responses in chronic undernutrition.

Fri Apr 29 00:00:00 EDT 2022

Cell fate determining molecular switches and signaling pathways in Pax7-expressing somitic mesoderm.

Cell discovery

Fung CW,Zhou S,Zhu H,Wei X,Wu Z,Wu AR

MA5-14889 was used in Immunocytochemistry-immunoflourescence to provide further understanding of the Pax7 lineage during embryonic development using a combination of lineage tracing with temporally sampled single-cell transcriptomics.

Tue Jun 28 00:00:00 EDT 2022

β-RA Targets Mitochondrial Metabolism and Adipogenesis, Leading to Therapeutic Benefits against CoQ Deficiency and Age-Related Overweight.

Biomedicines

Hidalgo-Gutiérrez A,Barriocanal-Casado E,Díaz-Casado ME,González-García P,Zenezini Chiozzi R,Acuña-Castroviejo D,López LC

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Wed Oct 13 00:00:00 EDT 2021

Defining the lineage of thermogenic perivascular adipose tissue.

Nature metabolism

Angueira AR,Sakers AP,Holman CD,Cheng L,Arbocco MN,Shamsi F,Lynes MD,Shrestha R,Okada C,Batmanov K,Susztak K,Tseng YH,Liaw L,Seale P

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Dermal Adipocyte Lipolysis and Myofibroblast Conversion Are Required for Efficient Skin Repair.

Cell stem cell

Shook BA,Wasko RR,Mano O,Rutenberg-Schoenberg M,Rudolph MC,Zirak B,Rivera-Gonzalez GC,López-Giráldez F,Zarini S,Rezza A,Clark DA,Rendl M,Rosenblum MD,Gerstein MB,Horsley V

MA5-14889 was used in Immunohistochemistry to identify an essential role for adipocyte lipolysis in regulating inflammation and repair after injury in skin.

Thu Jun 04 00:00:00 EDT 2020

Protective Effects of Foam Rolling against Inflammation and Notexin Induced Muscle Damage in Rats.

International journal of medical sciences

Pablos A,Ceca D,Jorda A,Rivera P,Colmena C,Elvira L,Martínez-Arnau FM,Valles SL

MA5-14889 was used in Western Blotting to conclude that applying foam rolling after damage has benefits such as an increase in anti-inflammatory proteins and a reduction of pro-inflammatory proteins, resulting in muscle recovery and better performance.

Mon Oct 05 00:00:00 EDT 2020

Protective Effects of Foam Rolling against Inflammation and Notexin Induced Muscle Damage in Rats.

International journal of medical sciences

Pablos A,Ceca D,Jorda A,Rivera P,Colmena C,Elvira L,Martínez-Arnau FM,Valles SL

Mon Oct 05 00:00:00 EDT 2020

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells.

BMC complementary and alternative medicine

Borah AK,Singh A,Yasmin R,Doley R,Mattaparthi VSK,Saha S

Published figure using PPAR gamma monoclonal antibody (Product # MA5-14889) in Western Blot

Fri Nov 29 00:00:00 EST 2019

Cross-talk between microtubules and the linker of nucleoskeleton complex plays a critical role in the adipogenesis of human adipose-derived stem cells.

Stem cell research & therapy

Yang Y,Qu R,Fan T,Zhu X,Feng Y,Yang Y,Deng T,Peng Y,Huang W,Ouyang J,Dai J

MA5-14889 was used in Western Blotting to determine the tole of mechanical force in the determination of lineage fate in adipose-derived stem cells.

Wed May 02 00:00:00 EDT 2018

A new hope for obesity management: Boron inhibits adipogenesis in progenitor cells through the Wnt/β-catenin pathway.

Metabolism: clinical and experimental

Doğan A,Demirci S,Apdik H,Bayrak OF,Gulluoglu S,Tuysuz EC,Gusev O,Rizvanov AA,Nikerel E,Şahin F

MA514889 was used in immunocytochemistry and western blot to investigate the effects of boron treatment on adipogenesis

Sat Apr 01 00:00:00 EDT 2017

Id1 Promotes Obesity by Suppressing Brown Adipose Thermogenesis and White Adipose Browning.

Diabetes

Patil M,Sharma BK,Elattar S,Chang J,Kapil S,Yuan J,Satyanarayana A

MA514889 was used in western blot to study the contribution of Id1 to brown adipose tissue thermogenesis and to the regulation of beige adipocytes in white adipose tissue

Thu Jun 01 00:00:00 EDT 2017

Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro.

Cytotechnology

Doğan A,Demirci S,Kıratlı B,Şahin F

MA514889 was used in immunocytochemistry and western blot to study the role of cytoglobin in adipogenesis.

Wed Feb 01 00:00:00 EST 2017

FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes.

Cell cycle (Georgetown, Tex.)

Liu L,Zheng LD,Zou P,Brooke J,Smith C,Long YC,Almeida FA,Liu D,Cheng Z

MA5-14889 was used in western blot to learn suppression of autophagy and lipid droplet growth in adipocytes by FoxO1 antagonist

Tue Aug 02 00:00:00 EDT 2016

DNA Topoisomerase IIα contributes to the early steps of adipogenesis in 3T3-L1 cells.

Cellular signalling

Jacobsen RG,Mazloumi Gavgani F,Mellgren G,Lewis AE

MA514889 was used in western blot to examine the role of topoisomerase II in mitotic clonal expansion and adipogenesis

Sat Oct 01 00:00:00 EDT 2016

Tamoxifen reduces fat mass by boosting reactive oxygen species.

Cell death & disease

Liu L,Zou P,Zheng L,Linarelli LE,Amarell S,Passaro A,Liu D,Cheng Z

MA5-14889 was used in western blot to study the effects of Tamoxifen administration on obesity

Thu Jan 08 00:00:00 EST 2015

Ebf2 is a selective marker of brown and beige adipogenic precursor cells.

Proceedings of the National Academy of Sciences of the United States of America

Wang W,Kissig M,Rajakumari S,Huang L,Lim HW,Won KJ,Seale P

MA5-14889 was used in immunocytochemistry to examine the expression of Ebf2 in adipogenic precursor cells

Tue Oct 07 00:00:00 EDT 2014

Targeting FoxO1 with AS1842856 suppresses adipogenesis.

Cell cycle (Georgetown, Tex.)

Zou P,Liu L,Zheng L,Liu L,Stoneman RE,Cho A,Emery A,Gilbert ER,Cheng Z

MA5-14889 was used in western blot to investigate the role of FoxO1 during adipocyte differentiation and adipogenesis

Thu Sep 10 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: HUMPPARG; Nuclear receptor subfamily 1 group C member 3; OTTHUMP00000185032; OTTHUMP00000185036; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; PPAR gamma; PPAR-gamma; PPARy

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Gene Aliases: CIMT1; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARgamma; PPARgamma2

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UniProt ID: (Human) P37231, (Mouse) P37238

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Entrez Gene ID: (Human) 5468, (Mouse) 19016

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Function(s)

core promoter sequence-specific DNA binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding identical protein binding sequence-specific DNA binding transcription regulatory region DNA binding retinoid X receptor binding arachidonic acid binding alpha-actinin binding RNA polymerase II regulatory region DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding metal ion binding WW domain binding C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PPAR gamma Monoclonal Antibody (K.242.9)

PPAR gamma Antibody (MA5-14889) in ICC/IF

Product Details

MA5-14889

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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