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Invitrogen
Prolactin Receptor Monoclonal Antibody (T6)
FIGURE: 1 / 6
Prolactin Receptor Antibody (MA1-611) in ICC/IF
Immunofluorescent analysis of Prolactin Receptor in H-4-II-E Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Prolactin Receptor monoclonal antibody (Product # MA1-611) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Prolactin Recept... View More
Product Details
MA1-611
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
T6
Immunogen
Purified rat liver PRL receptor.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2171289
Product Specific Information
MA1-611 detects prolactin (PRL) receptor in rat and human tissues. This antibody does not cross-react with PRL receptors from other species nor with growth hormone (GH) receptor.
MA1-611 has been successfully used in Western blot, IF, immunohistochemistry (4% PFA fixed tissue), and immunocytochemistry procedures. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells with MA1-611 yields a staining pattern consistent with cytoplasmic vesicular staining. MA1-611 has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
The MA1-611 immunogen is purified rat liver PRL receptor.
Reconstitute with 100 µL PBS.
Target Information
Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: hPRL receptor; Lactogen receptor; Prl R; PRL-R; Prolactin receptor; secreted prolactin binding protein
Gene Aliases: HPRL; hPRLrI; MFAB; PRLR; RATPRLR
UniProt ID: (Human) P16471, (Rat) P05710
Entrez Gene ID: (Human) 5618, (Rat) 24684
steroid biosynthetic process
cell surface receptor signaling pathway
activation of transmembrane receptor protein tyrosine kinase activity
embryo implantation
lactation
regulation of cell adhesion
regulation of epithelial cell differentiation
prolactin signaling pathway
T cell activation
activation of JAK2 kinase activity
negative regulation of apoptotic process
JAK-STAT cascade involved in growth hormone signaling pathway
mammary gland epithelial cell differentiation
prostate gland growth
mammary gland alveolus development
JAK-STAT cascade
cytokine-mediated signaling pathway
mammary gland epithelium development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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