Invitrogen

STAT5 alpha Monoclonal Antibody (ST5a-2H2)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

13 Published Figures

24 References

View all (104) STAT5 alpha antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

STAT5 alpha Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

STAT5 alpha Antibody (13-3600) in ICC/IF

Immunofluorescent analysis of STAT5 alpha was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT5 alpha Mouse Monoclonal Antibody (13-3600) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room tempera... View More

STAT5 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))

STAT5 alpha Antibody in Western Blot (WB)

STAT5 alpha Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

STAT5 alpha Antibody in Western Blot, ChIP Assay (WB, ChIP)

STAT5 alpha Antibody in Western Blot, Immunoprecipitation (WB, IP)

STAT5 alpha Antibody in ChIP Assay (ChIP)

Product Image Gallery

Product Details

13-3600

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Immunohistochemistry - Free Floating (IHC (Free))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1-3 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

5 µg

View 3 publications 3 publications

ChIP assay (ChIP)

View 3 publications 3 publications

Gel Shift (GS)

1-3 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Non-human primate, Rat, Tag, Virus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ST5a-2H2

Immunogen

A peptide corresponding to the C-terminus of murine Stat5a coupled to a protein carrier.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533013

Product Specific Information

This antibody recognizes the STAT5a protein from a variety of species including human, mouse, and rat and it reacts specifically with the STAT5a protein and does not exhibit any cross-reactivity with the highly related STAT5b protein. Cross-reactivity with other endogenous STAT isoforms has not been observed. Reactivity has been confirmed by western blotting using cell lysates derived from human A431 cells (+/- EGF treatment), NIH 3T3 cells, MCF-7 cells, and HeLa cells.

Target Information

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Drug target validation in primary human natural killer cells using CRISPR RNP.

Journal of leukocyte biology

Rautela J,Surgenor E,Huntington ND

13-3600 was used in Western Blot to show an efficient approach to genetically edit human NK cells by electroporation and CRISPR-Cas9 ribonucleoprotein complexes.

Thu Oct 01 00:00:00 EDT 2020

Deficiency of core fucosylation activates cellular signaling dependent on FLT3 expression in a Ba/F3 cell system.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Duan C,Fukuda T,Isaji T,Qi F,Yang J,Wang Y,Takahashi S,Gu J

13-3600 was used in Western Blotting to show that the N-glycan structures on wild type (WT), internal tandem duplication (ITD), and tyrosine kinase domain (TKD) mutants of FLT3 were different.

Sat Feb 01 00:00:00 EST 2020

Prospects for Clinical Development of Stat5 Inhibitor IST5-002: High Transcriptomic Specificity in Prostate Cancer and Low Toxicity In Vivo.

Cancers

Maranto C,Udhane V,Jia J,Verma R,Müller-Newen G,LaViolette PS,Pereckas M,Sabharwal L,Terhune S,Pattabiraman N,Njar VCO,Imig JD,Wang L,Nevalainen MT

13-3600 was used in Immunoprecipitation to support further optimization of IST5 for oral bioavailability for clinical development for therapies for solid tumors, hematological and myeloproliferative disorders.

Wed Nov 18 00:00:00 EST 2020

Identification of STAT3 and STAT5 proteins in the rat suprachiasmatic nucleus and the Day/Night difference in astrocytic STAT3 phosphorylation in response to lipopolysaccharide.

Journal of neuroscience research

Moravcová S,Červená K,Pačesová D,Bendová Z

13-3600 was used in immunohistochemistry (free floating) to examine the daily rhythm of STAT3 protein.

Fri Jan 01 00:00:00 EST 2016

Rapid Inflammation in Mice Lacking Both SOCS1 and SOCS3 in Hematopoietic Cells.

PloS one

Ushiki T,Huntington ND,Glaser SP,Kiu H,Georgiou A,Zhang JG,Metcalf D,Nicola NA,Roberts AW,Alexander WS

13-3600 was used in Western Blotting to study the role of the Suppressors of Cytokine Signalling (SOCS) proteins, SOCS1 and SOCS3, in the prevent of inflammatory disease.

Wed Aug 02 00:00:00 EDT 2017

DNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking.

PloS one

Nguyen M,Boutinaud M,Pétridou B,Gabory A,Pannetier M,Chat S,Bouet S,Jouneau L,Jaffrezic F,Laloë D,Klopp C,Brun N,Kress C,Jammes H,Charlier M,Devinoy E

13-3600 was used in EMSA to investigate the role of CpG methylation in milk production

Thu Jun 25 00:00:00 EDT 2015

A novel role of microRNA146b in promoting mammary alveolar progenitor cell maintenance.

Journal of cell science

Elsarraj HS,Hong Y,Valdez K,Carletti M,Salah SM,Raimo M,Taverna D,Prochasson P,Bharadwaj U,Tweardy DJ,Christenson LK,Behbod F

13-3600 was used in western blot to demonstrate that miR46b promotes the maintenance of pregnancy-derived mammary luminal alveolar progenitors.

Sat Jun 01 00:00:00 EDT 2013

Preoperative irradiation for the prevention of heterotopic ossification induces local inflammation in humans.

Bone

Hoff P,Rakow A,Gaber T,Hahne M,Sentürk U,Strehl C,Fangradt M,Schmidt-Bleek K,Huscher D,Winkler T,Matziolis D,Matziolis G,Badakhshi H,Burmester GR,Duda GN,Perka C,Buttgereit F

13-3600 was used in western blot to test if preoperative irradiation impacts local immune components.

Mon Jul 01 00:00:00 EDT 2013

Rac1 signalling modulates a STAT5/BCL-6 transcriptional switch on cell-cycle-associated target gene promoters.

Nucleic acids research

Barros P,Lam EW,Jordan P,Matos P

Published figure using STAT5 alpha monoclonal antibody (Product # 13-3600) in ChIP assay

Sat Sep 01 00:00:00 EDT 2012

Rac1 signalling modulates a STAT5/BCL-6 transcriptional switch on cell-cycle-associated target gene promoters.

Nucleic acids research

Barros P,Lam EW,Jordan P,Matos P

Published figure using STAT5 alpha monoclonal antibody (Product # 13-3600) in ChIP assay

Sat Sep 01 00:00:00 EDT 2012

Rac1 signalling modulates a STAT5/BCL-6 transcriptional switch on cell-cycle-associated target gene promoters.

Nucleic acids research

Barros P,Lam EW,Jordan P,Matos P

Published figure using STAT5 alpha monoclonal antibody (Product # 13-3600) in ChIP assay

Sat Sep 01 00:00:00 EDT 2012

A humanized pattern of aromatase expression is associated with mammary hyperplasia in mice.

Endocrinology

Zhao H,Pearson EK,Brooks DC,Coon JS,Chen D,Demura M,Zhang M,Clevenger CV,Xu X,Veenstra TD,Chatterton RT,DeMayo FJ,Bulun SE

13-3600 was used in western blot to use transgenic humanized aromatase mice to study the link between aromatase expression in mammary adipose tissue and breast pathology.

Fri Jun 01 00:00:00 EDT 2012

Prolactin signalling in the mouse hypothalamus is primarily mediated by signal transducer and activator of transcription factor 5b but not 5a.

Journal of neuroendocrinology

Yip SH,Eguchi R,Grattan DR,Bunn SJ

13-3600 was used in immunohistochemistry - free floating and western blot to report that STAT5b and not STAT5a is the primary mediator prolactin signaling in the hypothalamus.

Sat Dec 01 00:00:00 EST 2012

Integration of prolactin and glucocorticoid signaling at the beta-casein promoter and enhancer by ordered recruitment of specific transcription factors and chromatin modifiers.

Molecular endocrinology (Baltimore, Md.)

Kabotyanski EB,Huetter M,Xian W,Rijnkels M,Rosen JM

13-3600 was used in ChIP assay to examine the effects of prolactin and glucocorticoids on chromatin remodeling and transcriptional activation in mammary epithelial cells

Sun Oct 01 00:00:00 EDT 2006

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

Sat May 15 00:00:00 EDT 2004

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

Sat May 15 00:00:00 EDT 2004

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Cancer research

Silver DL,Naora H,Liu J,Cheng W,Montell DJ

Sat May 15 00:00:00 EDT 2004

The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5.

The Journal of biological chemistry

Gewinner C,Hart G,Zachara N,Cole R,Beisenherz-Huss C,Groner B

Fri Jan 30 00:00:00 EST 2004

The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5.

The Journal of biological chemistry

Gewinner C,Hart G,Zachara N,Cole R,Beisenherz-Huss C,Groner B

Fri Jan 30 00:00:00 EST 2004

Suppressor of cytokine signaling-1 is a critical regulator of interleukin-7-dependent CD8+ T cell differentiation.

Immunity

Chong MM,Cornish AL,Darwiche R,Stanley EG,Purton JF,Godfrey DI,Hilton DJ,Starr R,Alexander WS,Kay TW

Tue Apr 01 00:00:00 EST 2003

STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta.

The EMBO journal

Xu M,Nie L,Kim SH,Sun XH

Mon Feb 17 00:00:00 EST 2003

STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta.

The EMBO journal

Xu M,Nie L,Kim SH,Sun XH

Mon Feb 17 00:00:00 EST 2003

STAT5-induced Id-1 transcription involves recruitment of HDAC1 and deacetylation of C/EBPbeta.

The EMBO journal

Xu M,Nie L,Kim SH,Sun XH

Mon Feb 17 00:00:00 EST 2003

Hormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.

The Biochemical journal

Millot B,Montoliu L,Fontaine ML,Mata T,Devinoy E

13-3600 was used in Electrophoretic mobility shift assay to study the upstream regulatory regions of the mouse and rabbit whey acidic protein (WAP) genes to elucidate fully the mechanisms controlling WAP gene expression.

Thu May 15 00:00:00 EDT 2003

Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain.

Molecular biology of the cell

Saharinen P,Vihinen M,Silvennoinen O

Tue Apr 01 00:00:00 EST 2003

Autoinhibition of Jak2 tyrosine kinase is dependent on specific regions in its pseudokinase domain.

Molecular biology of the cell

Saharinen P,Vihinen M,Silvennoinen O

Tue Apr 01 00:00:00 EST 2003

Suppressor of cytokine signaling-1 regulates signaling in response to interleukin-2 and other gamma c-dependent cytokines in peripheral T cells.

The Journal of biological chemistry

Cornish AL,Chong MM,Davey GM,Darwiche R,Nicola NA,Hilton DJ,Kay TW,Starr R,Alexander WS

Fri Jun 20 00:00:00 EDT 2003

Cooperation among Stat1, glucocorticoid receptor, and PU.1 in transcriptional activation of the high-affinity Fc gamma receptor I in monocytes.

Journal of immunology (Baltimore, Md. : 1950)

Aittomäki S,Pesu M,Groner B,Jänne OA,Palvimo JJ,Silvennoinen O

13-3600 was used in immunoprecipitation and western blot to characterize the functional cooperation between IFN-gamma and dexamethasone in the induction of the high-affinity Fc gamma receptor I in monocytes.

Thu Jun 01 00:00:00 EDT 2000

Bioinformatics

Protein Aliases: Mammary gland factor; mammary gland factor STAT5A; signal transducer and activator of transcription 5; Signal transducer and activator of transcription 5A; STAT; STAT5B

View more View less

Gene Aliases: AA959963; MGF; Mpf; STAT5; STAT5A

View more View less

UniProt ID: (Human) P42229, (Rat) Q62771, (Mouse) P42230

View more View less

Entrez Gene ID: (Human) 6776, (Rat) 24918, (Mouse) 20850

View more View less

Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding protein tyrosine kinase activity signal transducer activity protein binding RNA polymerase II core promoter sequence-specific DNA binding double-stranded DNA binding protein phosphatase binding sequence-specific DNA binding DNA-binding transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

STAT5 alpha Monoclonal Antibody (ST5a-2H2)

STAT5 alpha Antibody (13-3600) in ICC/IF

Product Details

13-3600

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!