Proteintech

XBP1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (11)

XBP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

XBP1 Antibody (25997-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 25997-1-AP ( XBP1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

25997-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 40 publications 40 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

XBP1 Fusion Protein Ag21714 (167-261 aa encoded by BC000938)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LRLRAPLQQ VQAQLSPLQN ISPWILAVLT LQIQSLISCW AFWTTWTQSC SSNALPQSLP AWRSSQRSTQ KDPVPYQPPF LCQWGRHQPS WKPLMN (167-261 aa encoded by BC000938)

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deficiency of Perry syndrome-associated p150^Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.

NPJ Parkinson's disease

Yu J,Yang X,Zheng J,Sgobio C,Sun L,Cai H

Tue Mar 07 00:00:00 EST 2023

Tissue Proteogenomic Landscape Reveals the Role of Uncharacterized SEL1L3 in Progression and Immunotherapy Response in Lung Adenocarcinoma.

Journal of proteome research

Shen CY,Chang WH,Chen YJ,Weng CW,Regmi P,Kier MKK,Su KY,Chang GC,Chen JS,Chen YJ,Yu SL

Fri Apr 07 00:00:00 EDT 2023

The PERK/ATF4/CHOP signaling branch of the unfolded protein response mediates cisplatin-induced ototoxicity in hair cells.

Drug and chemical toxicology

Qu Y,Zong S,Wang Z,Du P,Wen Y,Li H,Wu N,Xiao H

Wed Mar 01 00:00:00 EST 2023

Shenlian extract protected ox-LDL-loaded macrophages against ER stress by promoting LAL-LXRα mediated cholesterol flux.

Journal of ethnopharmacology

Nie CX,Du XK,Yang LN,Li MJ,Liu L,Chen Y,Yang Q,Weng XG,Cai WY,Dong Y,Zhu XX,Li Q

Tue Dec 05 00:00:00 EST 2023

Shenlian extract protected ox-LDL-loaded macrophages against ER stress by promoting LAL-LXRα mediated cholesterol flux.

Journal of ethnopharmacology

Nie CX,Du XK,Yang LN,Li MJ,Liu L,Chen Y,Yang Q,Weng XG,Cai WY,Dong Y,Zhu XX,Li Q

Tue Dec 05 00:00:00 EST 2023

Tanshinone IIA ameliorates experimental diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress in cardiomyocytes via SIRT1.

Phytotherapy research : PTR

Wu S,Lu D,Gajendran B,Hu Q,Zhang J,Wang S,Han M,Xu Y,Shen X

Tue Aug 01 00:00:00 EDT 2023

TMBIM6 promotes diabetic tubular epithelial cell survival and albumin endocytosis by inhibiting the endoplasmic reticulum stress sensor, IRE1α.

Molecular biology reports

Xie H,Shi Y,Zhou Y,Liu H

Sat Oct 01 00:00:00 EDT 2022

A multi-omics approach identifies pancreatic cancer cell extracellular vesicles as mediators of the unfolded protein response in normal pancreatic epithelial cells.

Journal of extracellular vesicles

Hinzman CP,Singh B,Bansal S,Li Y,Iliuk A,Girgis M,Herremans KM,Trevino JG,Singh VK,Banerjee PP,Cheema AK

Wed Jun 01 00:00:00 EDT 2022

Artesunate Inhibits the Cell Growth in Colorectal Cancer by Promoting ROS-Dependent Cell Senescence and Autophagy.

Cells

Huang Z,Gan S,Zhuang X,Chen Y,Lu L,Wang Y,Qi X,Feng Q,Huang Q,Du B,Zhang R,Liu Z

Tue Aug 09 00:00:00 EDT 2022

Isovitexin protects against acute liver injury by targeting PTEN, PI3K and BiP via modification of m6A.

European journal of pharmacology

Huang Y,Chen S,Pang L,Feng Z,Su H,Zhu W,Wei J

Tue Feb 15 00:00:00 EST 2022

African Swine Fever Virus K205R Induces ER Stress and Consequently Activates Autophagy and the NF-κB Signaling Pathway.

Viruses

Wang Q,Zhou L,Wang J,Su D,Li D,Du Y,Yang G,Zhang G,Chu B

Tue Feb 15 00:00:00 EST 2022

Icariin attenuates ischaemic stroke through suppressing inflammation mediated by endoplasmic reticulum stress signalling pathway in rats.

Clinical and experimental pharmacology & physiology

Zheng J,Liao Y,Xu Y,Mo Z

Fri Jul 01 00:00:00 EDT 2022

TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease.

Cellular and molecular gastroenterology and hepatology

Xie M,Mak JWY,Yu H,Cheng CTY,Chan HCH,Chan TT,Lau LHS,Wong MTL,Ko WH,Jiang L,Yao X

Mon Jul 25 00:00:00 EDT 2022

Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.

Autophagy

Ming SL,Zhang S,Wang Q,Zeng L,Zhou LY,Wang MD,Ma YX,Han LQ,Zhong K,Zhu HS,Bai YL,Yang GY,Wang J,Chu BB

Mon Aug 01 00:00:00 EDT 2022

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Acta pharmaceutica Sinica. B

Yu W,Xu G,Chen H,Xiao L,Liu G,Hu P,Li S,Kasim V,Zeng C,Tong X

Sun May 01 00:00:00 EDT 2022

Comparison of multi-walled carbon nanotubes and halloysite nanotubes on lipid profiles in human umbilical vein endothelial cells.

NanoImpact

Liu Y,Hu Q,Huang C,Cao Y

Thu Jul 01 00:00:00 EDT 2021

MCLR induces dysregulation of calcium homeostasis and endoplasmic reticulum stress resulting in apoptosis in Sertoli cells.

Chemosphere

Zhao S,Yuan C,Tuo X,Zhou C,Zhao Q,Shen T

Fri Jan 01 00:00:00 EST 2021

Overexpression of lncRNA Dancr inhibits apoptosis and enhances autophagy to protect cardiomyocytes from endoplasmic reticulum stress injury via sponging microRNA-6324.

Molecular medicine reports

Li J,Xie J,Wang YZ,Gan YR,Wei L,Ding GW,Ding YH,Xie DX

Mon Feb 01 00:00:00 EST 2021

Zonisamide alleviates cardiac hypertrophy in rats by increasing Hrd1 expression and inhibiting endoplasmic reticulum stress.

Acta pharmacologica Sinica

Wu Q,Tian JH,He YX,Huang YY,Huang YQ,Zhang GP,Luo JD,Xue Q,Yu XY,Liu YH

Fri Oct 01 00:00:00 EDT 2021

EETs/sEHi alleviates nociception by blocking the crosslink between endoplasmic reticulum stress and neuroinflammation in a central poststroke pain model.

Journal of neuroinflammation

Liu T,Li T,Chen X,Li Z,Feng M,Yao W,Wan L,Zhang C,Zhang Y

Thu Sep 16 00:00:00 EDT 2021

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Redox biology

Wang Y,Chen J,Li S,Zhang X,Guo Z,Hu J,Shao X,Song N,Zhao Y,Li H,Yang G,Xu C,Wei C

Fri May 01 00:00:00 EDT 2020

Celecoxib attenuates hepatocyte apoptosis by inhibiting endoplasmic reticulum stress in thioacetamide-induced cirrhotic rats.

World journal of gastroenterology

Su W,Tai Y,Tang SH,Ye YT,Zhao C,Gao JH,Tuo BG,Tang CW

Tue Jul 28 00:00:00 EDT 2020

Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer.

Frontiers in oncology

Liu R,Wu Z,Zhang Y,Miao X,Zou Q,Yuan Y,Li D,Yang Z

Mon Sep 28 00:00:00 EDT 2020

Prognostic and Clinicopathological Significance of X-Box-Binding Protein 1 and N-Acetyltransferase 1 in Gallbladder Cancer.

Frontiers in oncology

Liu R,Wu Z,Zhang Y,Miao X,Zou Q,Yuan Y,Li D,Yang Z

Mon Sep 28 00:00:00 EDT 2020

Diazoxide Protects against Myocardial Ischemia/Reperfusion Injury by Moderating ERS via Regulation of the miR-10a/IRE1 Pathway.

Oxidative medicine and cellular longevity

Zhang L,Cai S,Cao S,Nie J,Zhou W,Zhang Y,Li K,Wang H,Yu S,Yu T

Fri May 14 00:00:00 EDT 2021

Paris saponin II-induced paraptosis-associated cell death increased the sensitivity of cisplatin.

Toxicology and applied pharmacology

Man S,Lv P,Cui J,Liu F,Peng L,Ma L,Liu C,Gao W

Sun Nov 01 00:00:00 EDT 2020

Minocycline induces apoptosis of photoreceptor cells by regulating ER stress.

Experimental eye research

Xu W,Tao M,Hu Z,Chang T,Wang Y

Wed Jan 01 00:00:00 EST 2020

miR-512-3p Overcomes Resistance to Cisplatin in Retinoblastoma by Promoting Apoptosis Induced by Endoplasmic Reticulum Stress.

Medical science monitor : international medical journal of experimental and clinical research

Kong M,Han Y,Zhao Y,Zhang H

Thu Jul 09 00:00:00 EDT 2020

Quantitative Proteomic Analysis Reveals Unfolded-Protein Response Involved in Severe Fever with Thrombocytopenia Syndrome Virus Infection.

Journal of virology

Zhang LK,Wang B,Xin Q,Shang W,Shen S,Xiao G,Deng F,Wang H,Hu Z,Wang M

Wed May 15 00:00:00 EDT 2019

Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity.

Molecular therapy. Nucleic acids

Xu W,Wu Y,Hu Z,Sun L,Dou G,Zhang Z,Wang H,Guo C,Wang Y

Fri Jun 07 00:00:00 EDT 2019

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway.

CNS neuroscience & therapeutics

Zhou MF,Feng ZP,Ou YC,Peng JJ,Li K,Gong HD,Qiu BH,Liu YW,Wang YJ,Qi ST

Wed May 01 00:00:00 EDT 2019

14-Deoxycoleon U-induced endoplasmic reticulum stress-mediated apoptosis, autophagy, and cell cycle arrest in lung adenocarcinoma.

OncoTargets and therapy

Peng X,Tu Y,Fu S,Xia Y,Ma C,Yang Y,Wu H,Liu Y,You P

Sun Apr 10 00:00:00 EDT 2022

Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.

Oncogene

Zhu Y,Xie M,Meng Z,Leung LK,Chan FL,Hu X,Chi K,Liu C,Yao X

Mon Jul 01 00:00:00 EDT 2019

Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells.

Journal of ophthalmology

Wu S,Zhu X,Guo B,Zheng T,Ren J,Zeng W,Chen X,Ke M

Fri Apr 08 00:00:00 EDT 2022

Unfolded Protein Response Pathways Correlatively Modulate Endoplasmic Reticulum Stress Responses in Rat Retinal Müller Cells.

Journal of ophthalmology

Wu S,Zhu X,Guo B,Zheng T,Ren J,Zeng W,Chen X,Ke M

Fri Apr 08 00:00:00 EDT 2022

Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Qin W,Wu X,Jia Y,Tong X,Guo C,Chen D,Wang Z,Tan N

Tue Oct 01 00:00:00 EDT 2019

Human placenta-derived mesenchymal stem cells inhibit apoptosis of granulosa cells induced by IRE1α pathway in autoimmune POF mice.

Cell biology international

Li H,Zhao W,Wang L,Luo Q,Yin N,Lu X,Hou Y,Cui J,Zhang H

Thu Aug 01 00:00:00 EDT 2019

Human placenta-derived mesenchymal stem cells inhibit apoptosis of granulosa cells induced by IRE1α pathway in autoimmune POF mice.

Cell biology international

Li H,Zhao W,Wang L,Luo Q,Yin N,Lu X,Hou Y,Cui J,Zhang H

Thu Aug 01 00:00:00 EDT 2019

Sulforaphane Improves Abnormal Lipid Metabolism via Both ERS-Dependent XBP1/ACC &SCD1 and ERS-Independent SREBP/FAS Pathways.

Molecular nutrition & food research

Tian S,Li B,Lei P,Yang X,Zhang X,Bao Y,Shan Y

Thu Mar 01 00:00:00 EST 2018

Sulforaphane Improves Abnormal Lipid Metabolism via Both ERS-Dependent XBP1/ACC &SCD1 and ERS-Independent SREBP/FAS Pathways.

Molecular nutrition & food research

Tian S,Li B,Lei P,Yang X,Zhang X,Bao Y,Shan Y

Thu Mar 01 00:00:00 EST 2018

Sulforaphane Improves Abnormal Lipid Metabolism via Both ERS-Dependent XBP1/ACC &SCD1 and ERS-Independent SREBP/FAS Pathways.

Molecular nutrition & food research

Tian S,Li B,Lei P,Yang X,Zhang X,Bao Y,Shan Y

Thu Mar 01 00:00:00 EST 2018

TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.

Journal of molecular and cellular cardiology

Li Z,Meng Z,Lu J,Chen FM,Wong WT,Tse G,Zheng C,Keung W,Tse K,Li RA,Jiang L,Yao X

Sun Jul 01 00:00:00 EDT 2018

TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.

Journal of molecular and cellular cardiology

Li Z,Meng Z,Lu J,Chen FM,Wong WT,Tse G,Zheng C,Keung W,Tse K,Li RA,Jiang L,Yao X

Sun Jul 01 00:00:00 EDT 2018

Asiatic acid from Potentilla chinensis alleviates non-alcoholic fatty liver by regulating endoplasmic reticulum stress and lipid metabolism.

International immunopharmacology

Wang D,Lao L,Pang X,Qiao Q,Pang L,Feng Z,Bai F,Sun X,Lin X,Wei J

Sat Dec 01 00:00:00 EST 2018

Asiatic acid from Potentilla chinensis alleviates non-alcoholic fatty liver by regulating endoplasmic reticulum stress and lipid metabolism.

International immunopharmacology

Wang D,Lao L,Pang X,Qiao Q,Pang L,Feng Z,Bai F,Sun X,Lin X,Wei J

Sat Dec 01 00:00:00 EST 2018

Farnesoid X receptor signaling activates the hepatic X-box binding protein 1 pathway in vitro and in mice.

Hepatology (Baltimore, Md.)

Liu X,Guo GL,Kong B,Hilburn DB,Hubchak SC,Park S,LeCuyer B,Hsieh A,Wang L,Fang D,Green RM

Sun Jul 01 00:00:00 EDT 2018

The reproductive toxicology of male SD rats after PM_2.5 exposure mediated by the stimulation of endoplasmic reticulum stress.

Chemosphere

Liu X,Jin X,Su R,Li Z

Fri Dec 01 00:00:00 EST 2017

The reproductive toxicology of male SD rats after PM_2.5 exposure mediated by the stimulation of endoplasmic reticulum stress.

Chemosphere

Liu X,Jin X,Su R,Li Z

Fri Dec 01 00:00:00 EST 2017

Herpes Simplex Virus 1 UL41 Protein Suppresses the IRE1/XBP1 Signal Pathway of the Unfolded Protein Response via Its RNase Activity.

Journal of virology

Zhang P,Su C,Jiang Z,Zheng C

Wed Feb 15 00:00:00 EST 2017

Herpes Simplex Virus 1 UL41 Protein Suppresses the IRE1/XBP1 Signal Pathway of the Unfolded Protein Response via Its RNase Activity.

Journal of virology

Zhang P,Su C,Jiang Z,Zheng C

Wed Feb 15 00:00:00 EST 2017

Exogenous H₂S restores ischemic post-conditioning-induced cardioprotection through inhibiting endoplasmic reticulum stress in the aged cardiomyocytes.

Cell & bioscience

Sun W,Yang J,Zhang Y,Xi Y,Wen X,Yuan D,Wang Y,Wei C,Wang R,Wu L,Li H,Xu C

Fri Mar 11 00:00:00 EST 2022

Exogenous H₂S restores ischemic post-conditioning-induced cardioprotection through inhibiting endoplasmic reticulum stress in the aged cardiomyocytes.

Cell & bioscience

Sun W,Yang J,Zhang Y,Xi Y,Wen X,Yuan D,Wang Y,Wei C,Wang R,Wu L,Li H,Xu C

Fri Mar 11 00:00:00 EST 2022

Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

Science advances

Huang C,Wu S,Ji H,Yan X,Xie Y,Murai S,Zhao H,Miyagishi M,Kasim V

Sun Oct 01 00:00:00 EDT 2017

Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

Science advances

Huang C,Wu S,Ji H,Yan X,Xie Y,Murai S,Zhao H,Miyagishi M,Kasim V

Sun Oct 01 00:00:00 EDT 2017

Identification of XBP1-u as a novel regulator of the MDM2/p53 axis using an shRNA library.

Science advances

Huang C,Wu S,Ji H,Yan X,Xie Y,Murai S,Zhao H,Miyagishi M,Kasim V

Sun Oct 01 00:00:00 EDT 2017

Hepatocyte X-box binding protein 1 deficiency increases liver injury in mice fed a high-fat/sugar diet.

American journal of physiology. Gastrointestinal and liver physiology

Liu X,Henkel AS,LeCuyer BE,Schipma MJ,Anderson KA,Green RM

Tue Dec 15 00:00:00 EST 2015

Hepatocyte X-box binding protein 1 deficiency increases liver injury in mice fed a high-fat/sugar diet.

American journal of physiology. Gastrointestinal and liver physiology

Liu X,Henkel AS,LeCuyer BE,Schipma MJ,Anderson KA,Green RM

Tue Dec 15 00:00:00 EST 2015

Bioinformatics

Protein Aliases: Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; XBP-1

View more View less

Gene Aliases: D11Ertd39e; HTF; TREB-5; TREB5; XBP-1; XBP1; XBP2

View more View less

UniProt ID: (Human) P17861, (Rat) Q9R1S4, (Mouse) O35426

View more View less

Entrez Gene ID: (Human) 7494, (Rat) 289754, (Mouse) 22433

View more View less

Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding protease binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

XBP1 Polyclonal Antibody

XBP1 Antibody (25997-1-AP) in IHC (P)

Product Details

25997-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!