Invitrogen

XBP1 Polyclonal Antibody

View all (26) XBP1 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-95907

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 277-376 of human XBP1 (NP_001073007.1).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.66 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2807709

Product Specific Information

Positive Samples: 293T, NCI-H460, Mouse eye, Rat liver, Rat eye; Cellular Location: Cytoplasm, Endoplasmic reticulum membrane, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein, Single-pass type II membrane protein

Immunogen sequence: NVVVKIEEAP LSPSENDHPE FIVSVKEEPV EDDLVPELGI SNLLSSSHCP KPSSCLLDAY SDCGYGGSLS PFSDMSSLLG VNHSWEDTFA NELFPQLISV

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; XBP-1

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Gene Aliases: D11Ertd39e; HTF; TREB-5; TREB5; XBP-1; XBP1; XBP2

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UniProt ID: (Human) P17861, (Rat) Q9R1S4, (Mouse) O35426

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Entrez Gene ID: (Human) 7494, (Rat) 289754, (Mouse) 22433

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding protease binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter angiogenesis liver development positive regulation of protein phosphorylation endothelial cell proliferation epithelial cell maturation positive regulation of immunoglobulin production regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter ubiquitin-dependent protein catabolic process positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response regulation of autophagy positive regulation of autophagy negative regulation of myotube differentiation phosphatidylinositol 3-kinase signaling cell growth endoplasmic reticulum unfolded protein response exocrine pancreas development positive regulation of histone methylation regulation of protein stability protein destabilization cellular response to nutrient positive regulation of TOR signaling cellular response to insulin stimulus cellular response to oxidative stress response to endoplasmic reticulum stress cellular triglyceride homeostasis cellular response to vascular endothelial growth factor stimulus cellular response to glucose starvation response to drug glucose homeostasis cholesterol homeostasis positive regulation of transcription factor import into nucleus negative regulation of apoptotic process positive regulation of MHC class II biosynthetic process positive regulation of B cell differentiation positive regulation of T cell differentiation positive regulation of fat cell differentiation positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway neuron development positive regulation of immunoglobulin secretion response to electrical stimulus fatty acid homeostasis sterol homeostasis serotonin secretion, neurotransmission adipose tissue development epithelial cell maturation involved in salivary gland development cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to antibiotic cellular response to fructose stimulus cellular response to glucose stimulus cellular response to interleukin-4 cellular response to peptide hormone stimulus cellular response to fluid shear stress cellular response to laminar fluid shear stress positive regulation of plasma cell differentiation negative regulation of endoplasmic reticulum unfolded protein response positive regulation of endoplasmic reticulum unfolded protein response positive regulation of proteasomal protein catabolic process positive regulation of protein acetylation negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway positive regulation of lactation response to insulin-like growth factor stimulus positive regulation of hepatocyte proliferation positive regulation of endothelial cell apoptotic process positive regulation of interleukin-6 secretion fatty acid biosynthetic process autophagy immune response muscle organ development protein transport IRE1-mediated unfolded protein response ATF6-mediated unfolded protein response intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress transcription, DNA-templated lipid metabolic process transport apoptotic process response to unfolded protein multicellular organism development cell differentiation
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