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XBP1 Antibody (MA5-52748) in WB
Western Blot analysis of XBP1. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XBP1 recombinant monoclonal antibody (Product # MA5-52748) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.
Product Details
MA5-52748
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB4840
Immunogen
A synthesized peptide derived from human XBP1 (120-160AA).
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Target Information
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: OTTHUMP00000028682; Tax-responsive element-binding protein 5; TREB-5; X-box-binding protein 1; XBP-1
Gene Aliases: TREB-5; TREB5; XBP-1; XBP1; XBP2
UniProt ID: (Human) P17861
Entrez Gene ID: (Human) 7494
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
phosphatidylinositol 3-kinase regulatory subunit binding
histone deacetylase binding
protein heterodimerization activity
angiogenesis
liver development
endothelial cell proliferation
positive regulation of immunoglobulin production
transcription from RNA polymerase II promoter
ubiquitin-dependent protein catabolic process
fatty acid biosynthetic process
autophagy
immune response
positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response
muscle organ development
regulation of autophagy
negative regulation of myotube differentiation
protein transport
endoplasmic reticulum unfolded protein response
exocrine pancreas development
protein destabilization
cellular response to nutrient
cellular response to insulin stimulus
cellular triglyceride homeostasis
IRE1-mediated unfolded protein response
ATF6-mediated unfolded protein response
cellular response to glucose starvation
cholesterol homeostasis
negative regulation of apoptotic process
positive regulation of MHC class II biosynthetic process
positive regulation of B cell differentiation
positive regulation of T cell differentiation
positive regulation of fat cell differentiation
positive regulation of transcription from RNA polymerase II promoter
neuron development
positive regulation of immunoglobulin secretion
fatty acid homeostasis
sterol homeostasis
adipose tissue development
epithelial cell maturation involved in salivary gland development
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
cellular response to fructose stimulus
cellular response to glucose stimulus
cellular response to interleukin-4
cellular response to peptide hormone stimulus
positive regulation of plasma cell differentiation
positive regulation of endoplasmic reticulum unfolded protein response
positive regulation of proteasomal protein catabolic process
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of lactation
response to insulin-like growth factor stimulus
positive regulation of hepatocyte proliferation
positive regulation of interleukin-6 secretion
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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