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XBP1S Polyclonal Antibody

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Antibody Testing Data (3)

XBP1S Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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XBP1S Antibody (24868-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

24868-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300

View 47 publications 47 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

XBP1 Fusion Protein Ag21703

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

293 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

Product Specific Information

Aliquoting is unnecessary for -20°C storage.

Target Information

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Genomic targets of the IRE1-XBP1s pathway in mediating metabolic adaptation in epithelial plasticity.

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Estrogens drive the endoplasmic reticulum-associated degradation and promote proto-oncogene c-Myc expression in prostate cancer cells by androgen receptor/estrogen receptor signaling.

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Frontiers in cell and developmental biology

Shen WB,Wang B,Yao R,Goetzinger KR,Wu S,Gao H,Yang P

Wed Mar 01 00:00:00 EST 2023

Obesity impacts placental function through activation of p-IRE1a-XBP1s signaling.

Frontiers in cell and developmental biology

Shen WB,Wang B,Yao R,Goetzinger KR,Wu S,Gao H,Yang P

Wed Mar 01 00:00:00 EST 2023

Transcriptomics reveals the effects of NTRK1 on endoplasmic reticulum stress response-associated genes in human neuronal cell lines.

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Triiodothyronine positively regulates endoplasmic reticulum-associated degradation (ERAD) and promotes androgenic signaling in androgen-dependent prostate cancer cells.

Cellular signalling

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Fri Sep 01 00:00:00 EDT 2023

Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu Z,Nan P,Gong Y,Tian L,Zheng Y,Wu Z

Tue Aug 01 00:00:00 EDT 2023

Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.

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Tue Aug 01 00:00:00 EDT 2023

Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.

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Tue Aug 01 00:00:00 EDT 2023

Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu Z,Nan P,Gong Y,Tian L,Zheng Y,Wu Z

Tue Aug 01 00:00:00 EDT 2023

Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.

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Tue Aug 01 00:00:00 EDT 2023

Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.

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Liu Z,Nan P,Gong Y,Tian L,Zheng Y,Wu Z

Tue Aug 01 00:00:00 EDT 2023

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Steroids

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Sat Jul 01 00:00:00 EDT 2023

Endoplasmic reticulum stress promotes sepsis-induced muscle atrophy via activation of STAT3 and Smad3.

Journal of cellular physiology

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Potent carotenoid astaxanthin expands the anti-cancer activity of cisplatin in human prostate cancer cells.

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Molecular biology reports

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Wed Feb 01 00:00:00 EST 2023

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Tissue & cell

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XBP1s gene of endoplasmic reticulum stress enhances proliferation and osteogenesis of human periodontal ligament cells.

Tissue & cell

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Tue Aug 01 00:00:00 EDT 2023

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Reproduction (Cambridge, England)

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Wed Feb 01 00:00:00 EST 2023

Endoplasmic reticulum stress promotes endometrial fibrosis through the TGF-β/SMAD pathway.

Reproduction (Cambridge, England)

Bao M,Feng Q,Zou L,Huang J,Zhu C,Xia W

Wed Feb 01 00:00:00 EST 2023

Endoplasmic reticulum stress promotes endometrial fibrosis through the TGF-β/SMAD pathway.

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Bao M,Feng Q,Zou L,Huang J,Zhu C,Xia W

Wed Feb 01 00:00:00 EST 2023

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The impairment of DDR reduces XBP1s, further increasing DNA damage, and triggers autophagy via PERK/eIF2alpha in MM and IRE1alpha/JNK1/2 in PEL cells.

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Sat Nov 26 00:00:00 EST 2022

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Sat Nov 26 00:00:00 EST 2022

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Exosomal DNAJB11 promotes the development of pancreatic cancer by modulating the EGFR/MAPK pathway.

Cellular & molecular biology letters

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Erbin protects against sepsis-associated encephalopathy by attenuating microglia pyroptosis via IRE1α/Xbp1s-Ca²⁺ axis.

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Sat Feb 26 00:00:00 EST 2022

Cold exposure-induced endoplasmic reticulum stress regulates autophagy through the SIRT2/FoxO1 signaling pathway.

Journal of cellular physiology

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Journal of ethnopharmacology

Hui Z,Wang S,Li J,Wang J,Zhang Z

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The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

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Yu W,Xu G,Chen H,Xiao L,Liu G,Hu P,Li S,Kasim V,Zeng C,Tong X

Sun May 01 00:00:00 EDT 2022

The P300/XBP1s/Herpud1 axis promotes macrophage M2 polarization and the development of choroidal neovascularization.

Journal of cellular and molecular medicine

Li W,Wang Y,Zhu L,Du S,Mao J,Wang Y,Wang S,Bo Q,Tu Y,Yi Q

Thu Jul 01 00:00:00 EDT 2021

Lipid Droplets Protect Human β-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes.

Diabetes

Tong X,Stein R

Mon Nov 01 00:00:00 EDT 2021

Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.

Cell calcium

Chen Y,Hu M,Deng F,Wang P,Lin J,Zheng Z,Liu Y,Dong L,Lu X,Chen Z,Zhou J,Zuo D

Wed Dec 01 00:00:00 EST 2021

Methamphetamine mediates apoptosis of vascular smooth muscle cells via the chop-related endoplasmic reticulum stress pathway.

Toxicology letters

Tan X,Cai D,Chen N,Du S,Qiao D,Yue X,Wang T,Li J,Xie W,Wang H

Sun Oct 10 00:00:00 EDT 2021

Overexpression of lncRNA Dancr inhibits apoptosis and enhances autophagy to protect cardiomyocytes from endoplasmic reticulum stress injury via sponging microRNA-6324.

Molecular medicine reports

Li J,Xie J,Wang YZ,Gan YR,Wei L,Ding GW,Ding YH,Xie DX

Mon Feb 01 00:00:00 EST 2021

Hydrogen sulphide reduces hyperhomocysteinaemia-induced endothelial ER stress by sulfhydrating protein disulphide isomerase to attenuate atherosclerosis.

Journal of cellular and molecular medicine

Jiang S,Xu W,Chen Z,Cui C,Fan X,Cai J,Gong Y,Geng B

Thu Apr 01 00:00:00 EDT 2021

Nox4 Promotes RANKL-Induced Autophagy and Osteoclastogenesis via Activating ROS/PERK/eIF-2α/ATF4 Pathway.

Frontiers in pharmacology

Sun J,Chen W,Li S,Yang S,Zhang Y,Hu X,Qiu H,Wu J,Xu S,Chu T

Sat Oct 16 00:00:00 EDT 2021

Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.

Nature communications

Yuan F,Jiang H,Yin H,Jiang X,Jiao F,Chen S,Ying H,Chen Y,Zhai Q,Guo F

Fri Jun 05 00:00:00 EDT 2020

Cell cycle exit during bortezomib-induced osteogenic differentiation of mesenchymal stem cells was mediated by Xbp1s-upregulated p21^Cip1 and p27^Kip1.

Journal of cellular and molecular medicine

Zhang D,Fan R,Lei L,Lei L,Wang Y,Lv N,Chen P,Williamson RA,Wang B,Hu J

Sat Aug 01 00:00:00 EDT 2020

Asiatic acid ameliorates acute hepatic injury by reducing endoplasmic reticulum stress and triggering hepatocyte autophagy.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Pang X,Qiao Q,Vonglorkham S,Feng Z,Pang L,Chen S,Wang D,Lao L,Lin X,Wei J

Tue Sep 01 00:00:00 EDT 2020

Salvianolic Acid B Protects Against Fatty Acid-Induced Renal Tubular Injury via Inhibition of Endoplasmic Reticulum Stress.

Frontiers in pharmacology

Mai X,Yin X,Chen P,Zhang M

Sat Jan 02 00:00:00 EST 2021

The novel autophagy inhibitor elaiophylin exerts antitumor activity against multiple myeloma with mutant TP53 in part through endoplasmic reticulum stress-induced apoptosis.

Cancer biology & therapy

Wang G,Zhou P,Chen X,Zhao L,Tan J,Yang Y,Fang Y,Zhou J

Thu Aug 03 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB-5; X-box-binding protein 1; XBP-1

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Gene Aliases: D11Ertd39e; TREB-5; TREB5; XBP-1; XBP1; XBP2

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UniProt ID: (Human) P17861, (Mouse) O35426

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Entrez Gene ID: (Human) 7494, (Mouse) 22433

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding phosphatidylinositol 3-kinase regulatory subunit binding histone deacetylase binding protein heterodimerization activity RNA polymerase II regulatory region sequence-specific DNA binding protease binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

XBP1S Polyclonal Antibody

XBP1S Antibody (24868-1-AP) in IHC (P)

Product Details

24868-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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