Invitrogen

alpha Tubulin Monoclonal Antibody (236-10501), Biotin

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Antibody Testing Data (6)

alpha Tubulin Antibody in Western Blot (WB)

FIGURE: 1 / 6

alpha Tubulin Antibody (A21371) in WB

Western blot analysis of alpha-Tubulin was performed by loading 20 µg of HCT 116 (lane1), MDA-MB-231 (lane2), Raji (lane3) and A431 (lane4) cell lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. alpha-Tubulin was detected at ~ 52 kDa using alpha-Tubulin Biotin Conjugated ... View More

alpha Tubulin Antibody in Immunocytochemistry (ICC/IF)

alpha Tubulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

alpha Tubulin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

A21371

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1.0 µg/mL

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

236-10501

Immunogen

Bovine Alpha-Tubulin.

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374524

Product Specific Information

Storage and reconstitution: upon receipt, store desiccated at -20ºC. To prepare a stock solution, reconstitute in PBS containing 1% BSA and 2mM sodium azide. Reconstituted solutions should remain stable for 6 months when stored at 4ºC. For longer storage, aliquot and store at -20ºC.

Target Information

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mapping the 3D orientation of piconewton integrin traction forces.

Nature methods

Brockman JM,Blanchard AT,Pui-Yan V,Derricotte WD,Zhang Y,Fay ME,Lam WA,Evangelista FA,Mattheyses AL,Salaita K

A21371 was used in Immunocytochemistry-immunoflourescence to describe molecular force microscopy to measure the magnitude and 3D orientation of cellular forces.

Thu Feb 01 00:00:00 EST 2018

Glycolytic requirement for NK cell cytotoxicity and cytomegalovirus control.

JCI insight

Mah AY,Rashidi A,Keppel MP,Saucier N,Moore EK,Alinger JB,Tripathy SK,Agarwal SK,Jeng EK,Wong HC,Miller JS,Fehniger TA,Mace EM,French AR,Cooper MA

A21371 was used in Immunocytochemistry-immunoflourescence to examine the role of glycolysis in natural killer (NK) cell function during murine cytomegalovirus infection and the ability of IL-15 to prime NK cells during cytomegalovirus infection.

Thu Dec 07 00:00:00 EST 2017

Analysis of the NK cell immunological synapse.

Methods in molecular biology (Clifton, N.J.)

Sanborn KB,Rak GD,Mentlik AN,Banerjee PP,Orange JS

A21371 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to study microscopy-based methods for evaluating and quantifying the NK cell immunological synapse that can be useful in enabling experimental studies.

Fri Feb 26 00:00:00 EST 2010

A single class II myosin modulates T cell motility and stopping, but not synapse formation.

Nature immunology

Jacobelli J,Chmura SA,Buxton DB,Davis MM,Krummel MF

A21371 was used in immunocytochemistry to analyze modulation of T cell motility and stopping, but not synapse formation by a single class II myosin

Sat May 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; TUBB; Tubulin alpha; tubulin alpha 6; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2B class IIb; tubulin, beta polypeptide; tubulin, beta polypeptide paralog

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Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3D; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; TUBB2B