Invitrogen

c-Met Monoclonal Antibody (eBioclone 7), PE, eBioscience™

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c-Met Antibody (12-8854-82) in Flow

Staining of mouse c-Met-transfected 293T cells with 0.25 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (open histogram) or 0.25 µg of Anti-Mouse c-Met (HGF Receptor) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-8854-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioclone 7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657816

Product Specific Information

Description: The eBioclone 7 monoclonal antibody was generated against a mouse c-Met-Ig fusion protein, and reacts with mouse c-Met-transfected cells. Mouse c-Met (HGFR) is a 170 kDa receptor tyrosine kinase (RTK) expressed by epithelial cells of the brain, kidney, liver and other tissues. Binding of its ligand, Hepatocyte Growth Factor (HGF), triggers receptor autophosphorylation, and activation of several downstream effectors including the mitogen-activated protein kinases ERK-1 and ERK-2, and PLC gamma. Activation of the c-Met signal transduction pathway leads to mulitple cellular responses including cell motility, scattering, proliferation, survival and angiogenesis. Mutations in human c-Met have been implicated in the development of several malignancies.

Applications Reported: This eBioclone 7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioclone 7 antibody has been tested by flow cytometric analysis of mouse c-Met-transfected 293T cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Hematopoietic Stem Cell-derived Adipocytes Promote Tumor Growth and Cancer Cell Migration.

International journal of cancer research and molecular mechanisms

Xiong Y,Russell DL,McDonald LT,Cowart LA,LaRue AC

12-8854 was used in Flow cytometry/Cell sorting to examine the role of haematopoietic stem cell-derived adipocytes in tumour progression.

Thu Oct 01 00:00:00 EDT 2020

Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma.

Oncotarget

Adachi E,Sakai K,Nishiuchi T,Imamura R,Sato H,Matsumoto K

12-8854 was used in Flow cytometry/Cell sorting to show that malignant melanoma has the ability to undergo phenotypic change by a cell-intrinsic/autonomous mechanism that can be characterised by Met expression.

Tue Oct 25 00:00:00 EDT 2016

Different growth and metastatic phenotypes associated with a cell-intrinsic change of Met in metastatic melanoma.

Oncotarget

Adachi E,Sakai K,Nishiuchi T,Imamura R,Sato H,Matsumoto K

Tue Oct 25 00:00:00 EDT 2016

Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice.

PloS one

Sakai H,Sato T,Sakurai H,Yamamoto T,Hanaoka K,Montarras D,Sehara-Fujisawa A

12-8854 was used in Flow cytometry/Cell sorting to demonstrate the potential of muscle progenitors for muscle repair and cell therapy after degenerative muscle disease.

Tue Dec 24 00:00:00 EST 2013

Convenient and efficient enrichment of the CD133+ liver cells from rat fetal liver cells as a source of liver stem/progenitor cells.

Stem cell reviews and reports

Liu WH,Li R,Dou KF

Tue Mar 01 00:00:00 EST 2011

c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.

Journal of immunology (Baltimore, Md. : 1950)

Gordin M,Tesio M,Cohen S,Gore Y,Lantner F,Leng L,Bucala R,Shachar I

Sun Aug 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: cMet; EC 2.7.10.1; hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene tyrosine kinase; oncogene MET; proto-oncogene c-Met; sc MET; scatter factor receptor; SF receptor; soluble c met; tyrosine-protein kinase Met

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Gene Aliases: AI838057; c-Met; HGF; HGFR; Par4

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Entrez Gene ID: (Mouse) 17295

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Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding ATP binding beta-catenin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding protein complex binding phospholipase binding phosphatidylinositol 3-kinase binding protein heterodimerization activity transmembrane signal receptor

Process(es)

Performance Guarantee

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c-Met Monoclonal Antibody (eBioclone 7), PE, eBioscience™

c-Met Antibody (12-8854-82) in Flow

Product Details

12-8854-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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