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c-Met (Cytoplasmic) Polyclonal Antibody

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Antibody Testing Data (12)

c-Met (Cytoplasmic) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

c-Met (Cytoplasmic) Antibody (25869-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 25869-1-AP) (c-Met (Cytoplasmic) antibody) at dilution of 1:1,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

c-Met (Cytoplasmic) Antibody in Western Blot (WB)

c-Met (Cytoplasmic) Antibody in Immunoprecipitation (IP)

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Product Details

25869-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

c-Met (Cytoplasmic) Fusion Protein Ag23140 (965-1085 aa encoded by BC130420)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

In Western blot, this antibody produces bands of unknown identity at 55 and 100 kDa.

Immunogen sequence: GSELVRYDA RVHTPHLDRL VSARSVSPTT EMVSNESVDY RATFPEDQFP NSSQNGSCRQ VQYPLTDMSP ILTSGDSDIS SPLLQNTVHI DLSALNPELV QAVQHVVIGP SSLIVHFNEV IG (965-1085 aa encoded by BC130420)

Target Information

MET (cMET) is a receptor-like tyrosine kinase whose dysregulation has been linked to many types of human malignancies. After activation of the ligand, MET interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, and STAT3. There interactions lead to the activation of signaling cascades including RAS-ERK, PI3, kinase-AKT, and PLCgamma-PKC. MET plays a role in embryonic development including gastrulation, development of muscles and neurons, angiogenesis, and kidney formation. It also plays a role in adults including wound healing, organ regeneration, and tissue remodeling. MET has been linked to cancers including gastric, renal, and breast; therefore, making it a target for cancer therapeutics and diagnostic testing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma.

International journal of experimental pathology

Zeng C,Wu JK,Lu X

Thu Jun 01 00:00:00 EDT 2023

Cytostatic Activity of Sanguinarine and a Cyanide Derivative in Human Erythroleukemia Cells Is Mediated by Suppression of c-MET/MAPK Signaling.

International journal of molecular sciences

Xu X,Deng L,Tang Y,Li J,Zhong T,Hao X,Fan Y,Mu S

Sun Apr 30 00:00:00 EDT 2023

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy.

Acta pharmacologica Sinica

Wu JC,Wang XJ,Zhu JH,Huang XY,Liu M,Qiao Z,Zhang Y,Sun Y,Wang ZY,Zhan P,Zhang T,Hu HL,Liu H,Tang W,Yi F

Thu Jun 01 00:00:00 EDT 2023

Identification and validation of autophagy-related gene expression for predicting prognosis in patients with idiopathic pulmonary fibrosis.

Frontiers in immunology

Huang G,Xu X,Ju C,Zhong N,He J,Tang XX

Tue Oct 11 00:00:00 EDT 2022

Time-extended exposure of gastric epithelial cells to secretome of Helicobacter pylori-activated fibroblasts induces reprogramming of gastric epithelium towards pre-cancerogenic and pro-invasive phenotype.

American journal of cancer research

Krzysiek-Maczka G,Targosz A,Wrobel T,Paw M,Szczyrk U,Opila J,Strzalka M,Wierdak M,Major P,Brzozowski T,Czyz J,Ptak-Belowska A

Wed Apr 13 00:00:00 EDT 2022

Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop.

Cell death & disease

Shen Y,Zhu Q,Xiao M,Yin L,Feng W,Feng J,He J,Li P,Chen X,Ding W,Zhong J,Zeng Z,Xie Z,Liu J,Zu X

Tue Aug 30 00:00:00 EDT 2022

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Cell death discovery

Song L,Zhang L,Zhou Y,Shao X,Xu Y,Pei D,Wang Q

Thu Apr 21 00:00:00 EDT 2022

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Cell death discovery

Song L,Zhang L,Zhou Y,Shao X,Xu Y,Pei D,Wang Q

Thu Apr 21 00:00:00 EDT 2022

A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma.

Frontiers in oncology

Wang H,Tian RF,Liang X,Fan J,Duan ZC,Fan XY,Zhang JJ,Yao DS,Chen ZN,Li L

Thu Feb 02 00:00:00 EST 2023

Trichostatin A downregulates bromodomain and extra-terminal proteins to suppress osimertinib resistant non-small cell lung carcinoma.

Cancer cell international

Meng Y,Qian X,Zhao L,Li N,Wu S,Chen B,Sun T,Wang X

Thu Apr 15 00:00:00 EDT 2021

Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors.

Investigational new drugs

Yuan X,Sun Z,Yuan Q,Hou W,Liang Q,Wang Y,Mo W,Wang H,Yu M

Mon Feb 01 00:00:00 EST 2021

Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.

Clinical and translational medicine

Dong X,Luo Y,Lu S,Ma H,Zhang W,Zhu Y,Sun G,Sun X

Mon Feb 01 00:00:00 EST 2021

Combinational blockade of MET and PD-L1 improves pancreatic cancer immunotherapeutic efficacy.

Journal of experimental & clinical cancer research : CR

Li E,Huang X,Zhang G,Liang T

Fri Sep 03 00:00:00 EDT 2021

Combinational blockade of MET and PD-L1 improves pancreatic cancer immunotherapeutic efficacy.

Journal of experimental & clinical cancer research : CR

Li E,Huang X,Zhang G,Liang T

Fri Sep 03 00:00:00 EDT 2021

Development of bispecific anti-c-Met/PD-1 diabodies for the treatment of solid tumors and the effect of c-Met binding affinity on efficacy.

Oncoimmunology

Yuan Q,Liang Q,Sun Z,Yuan X,Hou W,Wang Y,Wang H,Yu M

Fri Oct 15 00:00:00 EDT 2021

CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.

FEBS open bio

Zhao D,Yang Z,Chen C,Zhang Z,Yu Y,Li Z

Mon Nov 01 00:00:00 EDT 2021

Hepatocyte Growth Factor Overexpression Slows the Progression of 4NQO-Induced Oral Tumorigenesis.

Frontiers in oncology

He X,Chen S,Tang Y,Zhao X,Yan L,Wu L,Wu Z,Liu W,Chen X,Wang X

Fri Apr 29 00:00:00 EDT 2022

Combined Treatment of Cinobufotalin and Gefitinib Exhibits Potent Efficacy against Lung Cancer.

Evidence-based complementary and alternative medicine : eCAM

Han Y,Ma R,Cao G,Liu H,He L,Tang L,Li H,Luo Q

Tue Jun 15 00:00:00 EDT 2021

miRNA in food simultaneously controls animal viral disease and human tumorigenesis.

Molecular therapy. Nucleic acids

Cui Y,Wan H,Zhang X

Fri Mar 05 00:00:00 EST 2021

Development and Validation of a Nomogram based on cell growth-related Biomarkers for Oral Squamous Cell Carcinoma.

Journal of Cancer

Shuai Y,Duan Y,Zhou M,Yue K,Liu D,Fang Y,Wang Y,Wu Y,Zhang Z,Wang X

Tue Aug 03 00:00:00 EDT 2021

Effects of Cynaroside on Cell Proliferation, Apoptosis, Migration and Invasion though the MET/AKT/mTOR Axis in Gastric Cancer.

International journal of molecular sciences

Ji J,Wang Z,Sun W,Li Z,Cai H,Zhao E,Cui H

Tue Nov 09 00:00:00 EST 2021

Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.

International journal of biological sciences

Hou P,Lin T,Meng S,Shi M,Chen F,Jiang T,Li Z,Li M,Chu S,Zheng J,Bai J

Fri Mar 25 00:00:00 EDT 2022

Pulp stem cells with hepatocyte growth factor overexpression exhibit dual effects in rheumatoid arthritis.

Stem cell research & therapy

Dong X,Kong F,Liu C,Dai S,Zhang Y,Xiao F,Zhang H,Wu CT,Wang H

Wed Jun 10 00:00:00 EDT 2020

Increased expression of hematological and neurological expressed 1 (HN1) is associated with a poor prognosis of hepatocellular carcinoma and its knockdown inhibits cell growth and migration partly by down-regulation of c-Met.

The Kaohsiung journal of medical sciences

Chen JJ,Sun X,Mao QQ,Jiang XY,Zhao XG,Xu WJ,Zhong L

Sun Mar 01 00:00:00 EST 2020

Decorin promotes apoptosis and autophagy via suppressing c-Met in HTR-8 trophoblasts.

Reproduction (Cambridge, England)

Wang Y,Zhang H,Zhang Y,Li X,Hu X,Wang X

Fri May 01 00:00:00 EDT 2020

Tivantinib Decreases Hepatocyte Growth Factor-Induced BCRP Expression in Hepatocellular Carcinoma HepG2 Cells.

Biological & pharmaceutical bulletin

Kobayashi K,Higai K,Mukozu T,Matsui D,Amanuma M,Yoshimine N,Ogino Y,Matsui T,Wakui N,Shinohara M,Momiyama K,Daido Y,Nagai H,Igarashi Y

Wed Jun 16 00:00:00 EDT 2021

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Thoracic cancer

Chen Y,Tang J,Lu T,Liu F

Wed Jul 01 00:00:00 EDT 2020

Adipose-Derived Stem Cells Promote Proliferation and Invasion in Cervical Cancer by Targeting the HGF/c-MET Pathway.

Cancer management and research

Zhai Y,Wu W,Xi X,Yu R

Mon Apr 18 00:00:00 EDT 2022

CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.

Cell proliferation

Ying Y,Li J,Xie H,Yan H,Jin K,He L,Ma X,Wu J,Xu X,Fang J,Wang X,Zheng X,Liu B,Xie L

Sat Feb 01 00:00:00 EST 2020

Human-like collagen promotes the healing of acetic acid-induced gastric ulcers in rats by regulating NOS and growth factors.

Food & function

Xing M,Fu R,Liu Y,Wang P,Ma P,Zhu C,Fan D

Fri May 01 00:00:00 EDT 2020

The long noncoding RNA FTX promotes a malignant phenotype in bone marrow mesenchymal stem cells via the miR-186/c-Met axis.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Liu L,Li X,Shi Y,Chen H

Sun Nov 01 00:00:00 EDT 2020

The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance.

Autophagy

Huang X,Gan G,Wang X,Xu T,Xie W

Mon Jul 01 00:00:00 EDT 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 EST 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 EST 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 EST 2019

Dual regulatory role of CCNA2 in modulating CDK6 and MET-mediated cell-cycle pathway and EMT progression is blocked by miR-381-3p in bladder cancer.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Ying Y,Xie H,Jin K,Yan H,Wang S,Xu M,Xu X,Wang X,Yang K,Zheng X,Xie L

Tue Jan 01 00:00:00 EST 2019

A novel tetravalent bispecific antibody targeting programmed death 1 and tyrosine-protein kinase Met for treatment of gastric cancer.

Investigational new drugs

Hou W,Yuan Q,Yuan X,Wang Y,Mo W,Wang H,Yu M

Tue Oct 01 00:00:00 EDT 2019

Rosiglitazone Suppresses Calcium Oxalate Crystal Binding and Oxalate-Induced Oxidative Stress in Renal Epithelial Cells by Promoting PPAR-γ Activation and Subsequent Regulation of TGF-β1 and HGF Expression.

Oxidative medicine and cellular longevity

Liu YD,Yu SL,Wang R,Liu JN,Jin YS,Li YF,An RH

Mon Apr 20 00:00:00 EDT 2020

SEMA3C Promotes Cervical Cancer Growth and Is Associated With Poor Prognosis.

Frontiers in oncology

Liu R,Shuai Y,Luo J,Zhang Z

Thu Oct 01 00:00:00 EDT 2020

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 EDT 2018

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 EDT 2018

Clinicopathologic Features and Prognostic Factors in Alpha-Fetoprotein-Producing Colorectal Cancer: Analysis of 78 Cases.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Feng Y,Li Y,Dai W,Mo S,Li Q,Cai S

Fri Jan 25 00:00:00 EST 2019

Clinicopathologic Features and Prognostic Factors in Alpha-Fetoprotein-Producing Colorectal Cancer: Analysis of 78 Cases.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Feng Y,Li Y,Dai W,Mo S,Li Q,Cai S

Fri Jan 25 00:00:00 EST 2019

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Molecular therapy. Nucleic acids

Cui Y,Yang X,Zhang X

Fri Dec 15 00:00:00 EST 2017

Shrimp miR-34 from Shrimp Stress Response to Virus Infection Suppresses Tumorigenesis of Breast Cancer.

Molecular therapy. Nucleic acids

Cui Y,Yang X,Zhang X

Fri Dec 15 00:00:00 EST 2017

SOCS1 regulates hepatic regenerative response and provides prognostic makers for acute obstructive cholangitis.

Scientific reports

Yu J,Zhang W,Qian H,Tang H,Lin W,Lu B

Fri Aug 25 00:00:00 EDT 2017

SOCS1 regulates hepatic regenerative response and provides prognostic makers for acute obstructive cholangitis.

Scientific reports

Yu J,Zhang W,Qian H,Tang H,Lin W,Lu B

Fri Aug 25 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: c-Met; cMet; EC 2.7.10.1; Hepatocyte growth factor receptor; HGF receptor; HGF-SF receptor; HGF/SF receptor; met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; Met; protein tyrosin kinase; oncogene MET; Proto-oncogene c-Met; sc MET; Scatter factor receptor; SF receptor; soluble c met; Tyrosine-protein kinase Met

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Gene Aliases: AI838057; AUTS9; c-Met; DFNB97; HGF; HGFR; MET; Par4; RCCP2

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UniProt ID: (Dog) Q75ZY9, (Human) P08581, (Rat) P97523

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Entrez Gene ID: (Dog) 403438, (Human) 4233, (Rat) 24553, (Mouse) 17295

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Function(s)

ATP binding semaphorin receptor activity protein tyrosine kinase activity hepatocyte growth factor-activated receptor activity protein binding protein phosphatase binding protein kinase activity beta-catenin binding protein complex binding phospholipase binding phosphatidylinositol 3-kinase binding protein heterodimerization activity nucleotide binding transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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c-Met (Cytoplasmic) Polyclonal Antibody

c-Met (Cytoplasmic) Antibody (25869-1-AP) in IHC (P)

Product Details

25869-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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