BLOCK-iT™ RNAi Designer

The easiest way to design effective RNAi molecules for great results
See also:
Synthetics for in vivo RNAi
 Target Design Options:  Stealth RNAi™ siRNA miR RNAi shRNA siRNA to Stealth RNAi™ siRNA siRNA to shRNA
Step 1: Enter an accession number or provide a nucleotide sequence
Accession number:
Nucleotide sequence: Enter only A, C, G, T, and U. See the online Help for additional information
Step 2: If you entered an accession number in Step 1, select regions for target design
Open reading frame (ORF)
5' UTR
3' UTR
Step 3: Choose database for Blast
  NOTE: BLAST is used to compare input sequence with sequences in the database to find unique regions against which to design RNAi targets. The databases contain representative gene sequences for that species. Blast databases were updated on March 23, 2013 and the design output reflects the most up-to-date designs.
Step 4: Choose minimum and maximum G/C percentage
Minimum G/C percentage: Maximum G/C percentage:  
Step 5: Click "RNAi Design" to design your Stealth RNAi™ siRNA molecules.
Guarantee: The BLOCK-iT™ RNAi Designer is such an effective tool for the design of Stealth RNAi™ siRNA that if you order the three best Stealth RNAi™ siRNA sequences designed by the BLOCK-iT™ RNAi Designer, we guarantee that two of them will give greater than 70% knockdown of mRNA, given that the transfection efficiency in your experiment is at least 80%. If two or more fail to knock down your target RNA by at least 70% under these conditions, Invitrogen will design and ship a fourth Stealth RNAi™ siRNA to your target for free*.

*Please contact Invitrogen Technical Services to take advantage of this offer (800-955-6288 ext 2). Please be prepared to fax or email your order reference number, oligo sequences and data showing your transfection efficiency and knockdown. This offer is good for one free duplex per target.

How to Order: You must have an online account with Invitrogen to order custom oligos. After your oligos have been designed, you must enter a valid user name and password to send them to the ordering system. If you do not already have an account, click user registration or contact your local representative to set up an account.
If you already know your RNAi oligo sequence: Order oligos online at Order Custom Primers or by e-mail/Fax.