BLOCK-iT™ RNAi Designer
The easiest way to design effective RNAi molecules for great results   See also001: Synthetics for in vivo RNAi      Target Design Options:  Stealth RNAi™ siRNA miR RNAi shRNA siRNA to Stealth RNAi™ siRNA siRNA to shRNA
Convert siRNA to shRNA   To design shRNA based on existing siRNA for cloning into Invitrogen's vectors, enter the sense siRNA sequence, select one of the default loop sequences or enter a custom loop sequence, select appropriate vector and click 'Design shRNA Oligo'. Select pENTR™/H1/TO vector for inducible expression and pENTR™/U6 for constitutive expression.   Sense siRNA Sequence Default Loop Sequence Custom Loop Sequence Vector CGAA AACG GAGA pENTR™/H1/T0 pENTR™/U6