BLOCK-iT™ RNAi Designer

The easiest way to design effective RNAi molecules for great results

See also001:

Synthetics for in vivo RNAi

Target Design Options:

Stealth RNAi™ siRNA

siRNA to shRNA

Convert siRNA to shRNA


To design shRNA based on existing siRNA for cloning into Invitrogen's vectors, enter the sense siRNA sequence, select one of the default loop sequences or enter a custom loop sequence, select appropriate vector and click 'Design shRNA Oligo'. Select pENTR™/H1/TO vector for inducible expression and pENTR™/U6 for constitutive expression.

Sense siRNA Sequence	Default Loop Sequence	Custom Loop Sequence	Vector